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One-Pot Green Synthesis of Silver Nanoparticles Using the Orchid Leaf Extracts of Anoectochilus elatus: Growth Inhibition Activity on Seven Microbial Pathogens

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Abstract

The development of reliable antibiotics in the fight against multidrug resistant bacteria is a crucial challenge nowadays. Here, we describe a green chemistry approach for the synthesis of antimicrobial silver nanoparticles (AgNPs) employing the leaf extract of the orchid Anoectochilus elatus. Synthesized Ag NPs were studied by UV–visible and ATR-FT-IR spectroscopy, as well as by XRD, SEM and TEM. The UV–Visible spectra of AgNPs exhibited a surface plasmon resonance peak at 420 nm. XRD analysis showed that peaks at 38.09°, 44.59°, 64.67° and 77.54° confirmed the crystalline nature of AgNPs. TEM revealed spherical shapes with an average particle size of 20 nm. Synthesized AgNPs were screened to evaluate their antimicrobial activity against five Gram-negative and two Gram-positive human bacterial pathogens. AgNPs showed effective antibacterial activity against all tested bacterial strains at the concentration of 30–50 μL. Furthermore, the bactericidal efficacy of AgNPs can be useful to develop new eco-friendly antibacterial products to be used for photocatalytic, textile fabric applications, as well as to co-formulate detergents.

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Acknowledgements

The authors extend their sincere appreciations to the Deanship of Scientific Research at King Saud University for its funding this Prolific Research Group (PRG-1437-36). K. Gopinath sincerely thanks the KRIND Institute of Research and Development, Trichy, for supporting antibacterial assays.

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Authors and Affiliations

  1. Department of Botany, Bharathiar University, Coimbatore, Tamil Nadu, 641 046, India

    Kasi Gopinath & Natarajan Parimala Devi

  2. Unit of Vector Control, Phytochemistry and Nanotechnology, Department of Zoology, Annamalai University, Annamalainagar, Tamil Nadu, 608 002, India

    Marimuthu Govindarajan

  3. EMFT Division, CSIR- Central Electrochemical Research Institute, Karaikudi, Tamil Nadu, 630 003, India

    Kasi Bhakyaraj & Shanmugasundaram Kumaraguru

  4. Department of Botany, Alagappa University, Karaikudi, Tamil Nadu, 630 003, India

    Ayyakannu Arumugam

  5. Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia

    Naiyf S. Alharbi & Shine Kadaikunnan

  6. Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124, Pisa, Italy

    Giovanni Benelli

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  1. Kasi Gopinath
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  2. Natarajan Parimala Devi
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  3. Marimuthu Govindarajan
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  4. Kasi Bhakyaraj
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  5. Shanmugasundaram Kumaraguru
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  6. Ayyakannu Arumugam
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  7. Naiyf S. Alharbi
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  8. Shine Kadaikunnan
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  9. Giovanni Benelli
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Correspondence to Kasi Gopinath or Giovanni Benelli.

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Gopinath, K., Devi, N.P., Govindarajan, M. et al. One-Pot Green Synthesis of Silver Nanoparticles Using the Orchid Leaf Extracts of Anoectochilus elatus: Growth Inhibition Activity on Seven Microbial Pathogens. J Clust Sci 28, 1541–1550 (2017). https://doi.org/10.1007/s10876-017-1164-6

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  • DOI: https://doi.org/10.1007/s10876-017-1164-6

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