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Nitric oxide mediates hydrogen peroxide- and salicylic acid-induced salt tolerance in rice (Oryza sativa L.) seedlings

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Abstract

Nitric oxide (NO), hydrogen peroxide (H2O2), and salicylic acid (SA) are well-known signaling molecules that play multifaceted roles in the stress tolerance of plants; however, their interactions during stress alleviation have not been well studied. We investigated the possible regulatory role of NO in H2O2- and SA-induced reduction of oxidative damage in salt-exposed rice seedlings. For this purpose, hydroponically grown 14-day-old seedlings were pretreated with 100 μM H2O2 or 100 μM SA in the presence or absence of 100 μM hemoglobin (Hb, a potent NO scavenger) for 24 h followed by salt stress (200 mM NaCl) for 72 h. Salt stress significantly increased the levels of H2O2, malondialdehyde, and proline whereas H2O2 and SA pretreatment reduced the values of these parameters. H2O2 and SA pretreatment also inhibited salt-induced loss of total chlorophyll and relative water content. Histochemical detection of reactive oxygen species [ROS: superoxide (O ·−2 ) and H2O2] indicated evident oxidative burst in the seedlings stressed with salt alone. Salt stress modulated the non-enzymatic and enzymatic antioxidants differentially; however, H2O2 and SA treatment prior to salt stress enhanced these antioxidants compared with the salt-stressed seedlings alone. H2O2 and SA pretreated salt-stressed seedlings also showed higher induction of the methylglyoxal (MG) detoxification system. Endogenous NO content was elevated following H2O2 and SA pretreatment over the experimental period. Adding Hb reduced the level of NO and subsequently abolished the beneficial effects of H2O2 and SA. Our results, therefore, suggest that NO might be involved in H2O2- and SA-induced reduction of oxidative damage through the upregulation of the antioxidant defense and MG detoxification systems to confer salt tolerance in rice seedlings. The data are of considerable value in elucidating the biochemical mechanisms of salt-stress tolerance and will augment the goal of developing appropriate and efficient methods for crop protection in saline environment.

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Acknowledgments

M. G. Mostofa gratefully acknowledges the funding from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Laboratory of Plant Stress Responses, Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki, Kagawa, 761-0795, Japan

    Mohammad Golam Mostofa & Masayuki Fujita

  2. Department of Biochemistry and Molecular Biology, Bangabandhu Shiekh Mujibur Rahman Agricultural University, Gazipur, 1706, Bangladesh

    Mohammad Golam Mostofa

  3. Signaling Pathway Research Unit, RIKEN Center for Sustainable Resource Science, 1-7-22, Suehiro-cho, Tsurumi, Yokohama, 230-0045, Japan

    Lam-Son Phan Tran

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  1. Mohammad Golam Mostofa
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  2. Masayuki Fujita
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Correspondence to Masayuki Fujita or Lam-Son Phan Tran.

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Mostofa, M.G., Fujita, M. & Tran, LS.P. Nitric oxide mediates hydrogen peroxide- and salicylic acid-induced salt tolerance in rice (Oryza sativa L.) seedlings. Plant Growth Regul 77, 265–277 (2015). https://doi.org/10.1007/s10725-015-0061-y

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