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Enhancing salt tolerance in a crop plant by overexpression of glyoxalase II

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Abstract

Earlier we have shown the role of glyoxalase overexpression in conferring salinity tolerance in transgenic tobacco. We now demonstrate the feasibility of same in a crop like rice through overproduction of glyoxalase II. The rice glyoxalase II was cloned in pCAMBIA1304 and transformed into rice (Oryza sativa cv PB1) via Agrobacterium. The transgenic plants showed higher constitutive activity of glyoxalase II that increased further upon salt stress, reflecting the upregulation of endogenous glyoxalase II. The transgenic rice showed higher tolerance to toxic concentrations of methylglyoxal (MG) and NaCl. Compared with non-transgenics, transgenic plants at the T1 generation exhibited sustained growth and more favorable ion balance under salt stress conditions.

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Acknowledgments

We thank Professor Ray Wu, Cornell University, USA for valuable suggestions and critical reading of the manuscript. Thanks are also due to Drs. F White and BW Porter, Kansas State University, USA for the initial glyoxalase II clone and Drs. V. Rajamani and J. K. Tripathi, JNU, New Delhi for extending help in the work related to ionic content measurements. The financial support by the Department of Biotechnology (DBT, New Delhi) Rice Network Project, DBT Post-Doc fellowship to SKY and grants from the International Centre for Genetic Engineering and Biotechnology is duly acknowledged.

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  1. Sudesh Kumar Yadav

    Present address: Biotechnology Division, Institute of Himalayan Bioresource Technology, Palampur, 176061, Himachal Pradesh, India

Authors and Affiliations

  1. Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110 067, India

    Sneh L. Singla-Pareek, Sudesh Kumar Yadav, M. K. Reddy & S. K. Sopory

  2. Stress Physiology and Molecular Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110 067, India

    Ashwani Pareek

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  1. Sneh L. Singla-Pareek
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  2. Sudesh Kumar Yadav
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  3. Ashwani Pareek
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  4. M. K. Reddy
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  5. S. K. Sopory
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Correspondence to Sneh L. Singla-Pareek.

Additional information

Sneh L. Singla-Pareek and Sudesh Kumar Yadav have contributed equally to this work.

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Singla-Pareek, S.L., Yadav, S.K., Pareek, A. et al. Enhancing salt tolerance in a crop plant by overexpression of glyoxalase II. Transgenic Res 17, 171–180 (2008). https://doi.org/10.1007/s11248-007-9082-2

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  • DOI: https://doi.org/10.1007/s11248-007-9082-2

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