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Overexpression of GlyI and GlyII genes in transgenic tomato (Solanum lycopersicum Mill.) plants confers salt tolerance by decreasing oxidative stress

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Abstract

The glyoxalase system plays an important role in various physiological processes in plants, including salt stress tolerance. We report the effects of overexpressing glyoxalase I and glyoxalase II genes in transgenic tomato (Solanum lycopersicum Mill.) cv. Ailsa Craig. Stable expression of both transgenes was detected in the transformed tomato plants under salt stress. The transgenic lines overexpressing GlyI and GlyII under a high NaCl concentration (800 mM) showed reduced lipid peroxidation and the production of H2O2 in leaf tissues. A greater decrease in the chlorophyll a+b content in wild-type (WT) compared with transgenic lines was also observed. These results suggest that the over expression of two genes, GlyI and GlyII, may enhance salt stress tolerance by decreasing oxidative stress in transformed tomato plants. This work will help our understanding of the putative role of the glyoxalase system in the tolerance to abiotic stress in tomato plants.

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Acknowledgments

We are grateful to Dr. Sudhir K. Sopory, Plant Molecular Biology, International Center for Genetic Engineering & Biotechnology (ICGEB), New Delhi, India for providing the construction with two genes (GlyI and GlyII) of the glyoxalase pathway. This work was financially supported by the Fruit Consortium, Innova Corfo 11IDL2-10423 and the Millennium Nucleus for Plant Functional Genomics (P10-062-F).

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Authors and Affiliations

  1. Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Av. Alameda 340, P.O. Box 114-D, Santiago, Chile

    María Fernanda Álvarez Viveros & Patricio Arce-Johnson

  2. Núcleo de Investigación en Producción Alimentaria, Facultad de Recursos Naturales, Escuela de Agronomía, Universidad Católica de Temuco, P.O. Box 56-D, Temuco, Chile

    Claudio Inostroza-Blancheteau

  3. Facultad de Ingeniería y Ciencias, Universidad Adolfo Ibáñez, Av. Diagonal Las Torres 2640, Santiago, Chile

    Tania Timmermann

  4. Facultad de Agronomía e Ingeniería Forestal, Pontificia Universidad Católica de Chile, Av. Vicuña Mackenna 4560, Santiago, Chile

    María Fernanda Álvarez Viveros & Máximo González

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  1. María Fernanda Álvarez Viveros
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  2. Claudio Inostroza-Blancheteau
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Correspondence to Patricio Arce-Johnson.

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Álvarez Viveros, M.F., Inostroza-Blancheteau, C., Timmermann, T. et al. Overexpression of GlyI and GlyII genes in transgenic tomato (Solanum lycopersicum Mill.) plants confers salt tolerance by decreasing oxidative stress. Mol Biol Rep 40, 3281–3290 (2013). https://doi.org/10.1007/s11033-012-2403-4

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  • DOI: https://doi.org/10.1007/s11033-012-2403-4

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