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Edaphocola aurantiacus gen. nov., sp. nov., a new member of the family Chitinophagaceae isolated from wetland soil in South Korea

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Abstract

A short rod-shaped, yellow–orange pigmented, strictly aerobic bacterium, designated as strain H2T, was isolated from the wetland soil of Halla Mountain, Jeju-island, South Korea. Growth was observed at temperatures of 10–30 °C (optimum at 25–30 °C), pH of 6–8 (optimum at pH 7), and salt concentrations of 0–1% (w/v) NaCl (optimum at 0%). The strain H2T was found to be a catalase and oxidase-positive, non-motile, Gram-negative bacterium. On the basis of 16S rRNA gene sequence similarity and phylogenetic analysis, strain H2T was found to be related to the members of the Chitinophagaceae family, being closely related to Taibaiella chishuiensis AY17T (94.3% sequence similarity). The major polar lipids are phosphatidylethanolamine and glycolipid. Strain H2T contained MK-7 as the only menaquinone as well as iso-C15:0, iso-C15:1 G and iso-C17:0 3-OH as the major fatty acids (> 15%). The DNA G+C content of strain H2T was determined to be 48.3 mol%. Based on the phylogenetic, phenotypic characteristics and chemotaxonomic analysis data, strain H2T (= KCTC 62115T = JCM 32353T) should be classified as representative of a novel species of a novel genus within the family Chitinophagaceae, for which the name Edaphocola aurantiacus gen. nov., sp. nov., is proposed.

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Acknowledgements

We are grateful to Dr. Bernhard Schink for helping us with etymology. This research was supported by the National Research Foundation of Korea (NRF) Grant funded by the Korea government (MIST) (NRF-2017R1A2B4009448).

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Authors and Affiliations

  1. Department of Life Science, Dongguk University-Seoul, Goyang, 10326, South Korea

    Jiwon Choi, Seho Cha, Geeta Chhetri, Dahye Yang & Taegun Seo

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  1. Jiwon Choi
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  2. Seho Cha
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  3. Geeta Chhetri
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  4. Dahye Yang
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  5. Taegun Seo
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Contributions

JC isolated the bacterium, designed the study, performed the phenotypic and biochemical characterization, wrote the original draft; SC, GC and DY helped the analysis of taxonomic data; TS designed and supervised the study, edited the original draft.

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Correspondence to Taegun Seo.

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Choi, J., Cha, S., Chhetri, G. et al. Edaphocola aurantiacus gen. nov., sp. nov., a new member of the family Chitinophagaceae isolated from wetland soil in South Korea. Antonie van Leeuwenhoek 112, 687–694 (2019). https://doi.org/10.1007/s10482-018-1199-1

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