Skip to main content
SpringerLink
Log in

Flavisolibacter galbus sp. nov., isolated from soil in Jeju Island

  • Original Paper
  • Published:
Antonie van Leeuwenhoek Aims and scope Submit manuscript

Abstract

A Gram-stain negative, non-motile, and yellow-coloured bacterium, designated 17J28-26T, was isolated from soil in Jeju Island, Korea. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain 17J28-26T formed a distinct lineage within the family Chitinophagaceae (order Chitinophagales, class Chitinophagia), and is closely related to Flavisolibacter ginsenosidimutans (96.8% 16S rRNA gene sequence similarity), and Flavisolibacter ginsengisoli (96.6%). Growth was observed at 18–37 °C (optimum 30 °C) in R2A medium at pH 7.0. The major cellular fatty acids of strain 17J28-26T were summed feature 3 (C16:1 ω6c and/or C16:1 ω7c), and iso-C15:0. The predominant respiratory quinone was MK-7. The major polar lipid was identified as phosphatidylethanolamine. Based on biochemical, chemotaxonomic and phylogenetic characteristics, strain 17J28-26T represents a novel bacterial species within the family Chitinophagaceae, for which the name Flavisolibacter galbus sp. nov. is proposed. The type strain of Flavisolibacter galbus is 17J28-26T (= KCTC 62222T = JCM 33203T).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

Abbreviations

L:

Lipid

PL:

Phospholipid

APL:

Aminophospholipid

PE:

Phosphatidylethanolamine (PE)

References

  • Baik KS, Kim MS, Lee JH, Lee SS, Im WT, Seong CN (2014) Flavisolibacter rigui sp. nov., isolated from freshwater of an artificial reservoir and emended description of the genus Flavisolibacter. Int J Syst Evol Microbiol 64:4038–4042

    Article  CAS  PubMed  Google Scholar 

  • Bates RG, Bower VE (1956) Alkaline solutions for pH control. Anal Chem 28:1322–1324

    Article  CAS  Google Scholar 

  • Cappuccino JG, Sherman N (2002) Microbiology- A laboratory manual, 6th edn. Pearson Education, Inc. Benjamin Cummings, California

    Google Scholar 

  • Felsenstein J (1985) Confidence limit on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  PubMed  Google Scholar 

  • Fitch MW (1971) Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zoo 20:406–416

    Article  Google Scholar 

  • Gomori G (1955) Preparation of buffers for use in enzyme studies. Methods Enzymol 1:138–146

    Article  CAS  Google Scholar 

  • Hiraishi A, Ueda Y, Ishihara J, Mori T (1996) Comparative lipoquinone analysis of influent sewage and activated sludge by high performance liquid chromatography and photodiode array detection. J Gen Appl Microbiol 42:457–469

    Article  CAS  Google Scholar 

  • Jeon YS, Lee K, Park SC, Kim BS, Cho YJ, Ha SM, Chun J (2014) EzEditor: a versatile sequence alignment editor for both rRNA- and protein-coding genes. Int J Syst Evol Microbiol 64:689–691

    Article  CAS  PubMed  Google Scholar 

  • Kämpfer P, Lodders N, Falsen E (2011) Hydrotalea flava gen. nov., sp. nov., a new member of the phylum Bacteroidetes and allocation of the genera Chitinophaga, Sediminibacterium, Lacibacter, Flavihumibacter, Flavisolibacter, Niabella, Niastella, Segetibacter, Parasegetibacter, Terrimonas, Ferruginibacter, Filimonas and Hydrotalea to the family Chitinophagaceae fam. nov. Int J Syst Evol Microbiol 61:518–523

    Article  CAS  PubMed  Google Scholar 

  • Kim MK, Im W-T, Ohta H, Lee M, Lee S-T (2005) Sphingopyxis granuli sp. nov., a β-glucosidase-producing bacterium in the family Sphingomonadaceae in a-4 subclass of the Proteobacteria. J Microbiol 43:152–157

    CAS  PubMed  Google Scholar 

  • Kim DU, Lee H, Lee S, Kim SG, Park AY, Ahn JH, Ka JO (2018) Flavisolibacter metallilatus sp. nov., isolated from an automotive air conditioning system and emended description of the genus Flavisolibacter. Int J Syst Evol Microbiol 68:917–923

    Article  CAS  PubMed  Google Scholar 

  • Komagata K, Suzuki K (1987) Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207

    Article  CAS  Google Scholar 

  • Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mole Bio Evol 33:1870–1874

    Article  CAS  Google Scholar 

  • Lee J, Kang M, Kim G, Lee C, Lim S, Lee J, Roh S, Kang H, Ha J, Bae S, Jung H, Kim M (2016) Flavisolibacter tropicus sp. nov., isolated from tropical soil. Int J Syst Evol Microbiol 66:3413–3419

    Article  CAS  PubMed  Google Scholar 

  • Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M, Schaal A, Parlett JH (1984) An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241

    Article  CAS  Google Scholar 

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  PubMed  Google Scholar 

  • Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101. Newark, DE: MIDI Inc

  • Stackebrandt E, Goebel BM (1994) Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 44:846–849

    Article  CAS  Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24:4876–4882

    Article  Google Scholar 

  • Weisburg WG, Barns SM, Pellerier DA, Lane DJ (1991) 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Yoon MH, Im WT (2007) Flavisolibacter ginsengiterrae gen. nov., sp. nov. and Flavisolibacter ginsengisoli sp. nov., isolated from ginseng cultivating soil. Int J Syst Evol Microbiol 57:1834–1839

    Article  CAS  PubMed  Google Scholar 

  • Yoon SH, Ha SM, Kwon S, Lim J, Kim Y, Seo H, Chun J (2017) Introducing EzBioCloud: a taxonomically united database of 16S rRNA and whole genome assemblies. Int J Syst Evol Microbiol 67:1613–1617

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zhao Y, Liu Q, Kang MS, Jin F, Yu H, Im WT (2015) Flavisolibacter ginsenosidimutans sp. nov., with ginsenoside-converting activity isolated from soil used for cultivating ginseng. Int J Syst Evol Microbiol 65:4868–4872

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgement

This work was supported by a research grant from Seoul Women’s University (2018) and by a grant from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR201801204).

Author information

Authors and Affiliations

  1. Department of Public Health Sciences, Graduate School, Korea University, Seoul, 02841, Republic of Korea

    Soo Hyun Maeng & Hana Yi

  2. Department of Bio & Environmental Technology, College of Natural Science, Seoul Women’s University, Seoul, 139-774, Korea

    Myung Kyum Kim & Gayathri Subramani

  3. School of Applied Biosciences, Department of Horticultural Science, Institute of Plant Medicine, Kyungpook National University, Daegu, 41566, Republic of Korea

    Jun Hwee Jang

  4. School of Biosystem and Biomedical Science, Korea University, Seoul, 02841, Korea

    Hana Yi

Authors
  1. Soo Hyun Maeng
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Myung Kyum Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jun Hwee Jang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hana Yi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Gayathri Subramani
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

All authors equally contributed in this work.

Corresponding author

Correspondence to Gayathri Subramani.

Ethics declarations

Conflict of interest

All authors certify that there is no conflict of interest with any financial organization regarding the material discussed in the manuscript.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Maeng, S.H., Kim, M.K., Jang, J.H. et al. Flavisolibacter galbus sp. nov., isolated from soil in Jeju Island. Antonie van Leeuwenhoek 112, 1559–1565 (2019). https://doi.org/10.1007/s10482-019-01282-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10482-019-01282-8

Keywords

Search

Navigation