Abstract
A novel bacterial strain, designated as THG-SC4T, was isolated from a soil sample collected from Yongin city in South Korea. Cells of the strain were Gram-negative, aerobic, rod-shaped and non-motile. The strain grew optimally at 28–30 °C; at pH 7.0 and in the absence of NaCl. Flexirubin-type pigments were found to be present. On the basis of 16S rRNA gene sequence similarity, strain THG-SC4T was shown to belong to the genus Taibaiella and shares high sequence similarity with Taibaiella smilacinae KCTC 32316T (95.4 %), followed by Taibaiella koreensis KACC 17171T (94.3 %) and Taibaiella chishuiensis JCM 19637T (94.2 %). The DNA G+C content of the novel isolate was determined to be 43.1 mol% and MK-7 was identified as the predominant isoprenoid quinone. The only polyamine was homospermidine. The major polar lipids were phosphatidylethanolamine, an unidentified aminophospholipid and an unidentified polar lipid. The predominant fatty acids were identified as iso-C15:0, iso-C15:1 G, C16:0 and iso-C17:03-OH. On the basis of data from this polyphasic taxonomic study, strain THG-SC4T is considered to represent a novel species of the genus Taibaiella, for which the name Taibaiella yonginensis sp. nov. is proposed. The type strain is THG-SC4T (=KACC 18372T = CCTCC AB 2014316T).
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Acknowledgments
This work was conducted under the industrial infrastructure program (No. N0000888) for fundamental technologies which is funded by the Ministry of Trade, Industry & Energy (MOTIE, Korea).
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The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain THG-SC4T is KM598262.
Hina Singh and Juan Du have contributed equally to this work.
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10482_2015_505_MOESM1_ESM.tif
Supplementary Fig. S1. The Maximum-likelihood tree based on 16S rRNA gene sequence analysis showing phylogenetic relationships of strain THG-SC4T and other related type species. Evolutionary distances were computed using Tamura-Nei model. Numbers at nodes represent percentages of bootstrap support based on a maximum-likelihood analysis of 1000 resampled datasets. Bootstrap values more than 50 % are shown at branching points. Burkholderia cepacia ATCC 25416T was used as an outgroup. Bar, 0.2 nucleotide substitutions per nucleotide position
10482_2015_505_MOESM2_ESM.tif
Supplementary Fig. S2. Transmission electron micrographs of Taibaiella yonginensis THG-SC4T after negative staining with uranyl acetate. Bar indicated 0.5 μm
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Singh, H., Du, J., Won, K. et al. Taibaiella yonginensis sp. nov., a bacterium isolated from soil of Yongin city. Antonie van Leeuwenhoek 108, 517–524 (2015). https://doi.org/10.1007/s10482-015-0505-4
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DOI: https://doi.org/10.1007/s10482-015-0505-4