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Physiological and biochemical parameters influencing ex vitro establishment of the in vitro regenerants of Albizia lebbeck (L.) Benth.: an important soil reclaiming plantation tree

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Abstract

Present approach demonstrates a speedy and competent in vitro regeneration method for A. lebbeck. The hypocotyl explants (HP) excised from a 15-day-old seedlings were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations (0.5, 2.5, 5.0, 7.5, 10.0 and 12.5 μM) of 6-Benzyladenine (BA), Kinetin (Kn) and 2-Isopentenyl adenine (2-iP) singly as well as in combination with α-naphthalene acetic acid (NAA) or Indole-3-butyric acid (IBA) or Indole-3-acetic acid (IAA; 0.1, 0.5, 1.0, 1.5 and 2.0 μM). The best shoot regeneration rate (88 %) with number of shoots (34.00 ± 1.15) and shoot length (6.30 ± 0.05 cm) per explant was obtained on MS + BA (7.5 μM) + NAA (0.5 μM) after 8 weeks of culture. While, the explants cultured onto the MS + TDZ (Thidiazuron; 0.5, 1.0, 2.5, 5.0 and 7.5 μM) singly showed maximum shoot proliferation response of 77 % when, TDZ exposed cultures were transferred on the BA (7.5 µM) + NAA (0.5 µM) supplied MS medium producing maximum no. of shoots (20.30 ± 0.32) and shoot length (5.15 ± 0.08 cm) after 8 weeks of culture. The regenerated shoots were rooted by using ex vitro rooting method and successfully acclimatized by using a simple acclimatization procedure of 70 days, primarily in soilrite for 28 days and finally in soilrite + garden soil (1:1) for 70 days under culture room conditions (150 PPFD). This acclimatization period under the optimized conditions of culture room ensures a high survival rate (80 %) of the plantlets in the field conditions. Photosynthetic pigments (Chlorophyll a, b and carotenoid content) evaluated during acclimatization period showed a decreasing trend in the initial 14 days afterwards a significant increase in the subsequent period of 21–70 days. While all the stress parameters MDA and H2O2 content showed decreasing trend all through the acclimatization, concurrently all the tested antioxidative enzymes (SOD, CAT, APX and GR) exhibited an increasing trend.

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Abbreviations

BA:

6-Benzyladenine

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

2iP:

2-Isopentenyl adenine

Kn:

6-Furfurylaminopurine

PGRs:

Plant growth regulators

MS:

Murashige and Skoog (1962) medium

NAA:

α-Naphthalene acetic acid

TDZ:

Thidiazuron

HP:

Hypocotyl explant

Chl:

Chlorophyll

Car:

Carotenoid

PN :

Photosynthetic rate

SOD:

Superoxide dismutase

CAT:

Catalase

APX:

Ascorbate peroxidase

GR:

Glutathione reductase

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Acknowledgments

Financial support from the DST-FIST (2011–2016) and UGC (DRS-I) (2009–2014) programmes, Govt of India, New Delhi, is highly appreciated.

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  1. Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, Aligarh, 202 002, India

    S. Perveen & M. Anis

  2. Department of Plant Production, College of Food and Agricultural Sciences, King Saud University, Riyadh, 11451, Saudi Arabia

    M. Anis

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Perveen, S., Anis, M. Physiological and biochemical parameters influencing ex vitro establishment of the in vitro regenerants of Albizia lebbeck (L.) Benth.: an important soil reclaiming plantation tree. Agroforest Syst 89, 721–733 (2015). https://doi.org/10.1007/s10457-015-9809-7

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