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Transient expression of glyoxal oxidase from the Chinese wild grape Vitis pseudoreticulata can suppress powdery mildew in a susceptible genotype

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Abstract

Vitis pseudoreticulata glyoxal oxidase (VpGLOX) was previously isolated from the Chinese wild vine V. pseudoreticulata accession “Baihe-35-1” during a screen for genes that are upregulated in response to infection with grapevine powdery mildew (Erysiphe necator, PM). In the present study, a possible function of VpGLOX for defense against PM was investigated using Agrobacterium-mediated transient expression. After optimizing agro-infiltration, VpGLOX was transiently overexpressed in leaves of either PM-susceptible (accession “6-12-2”) or PM-resistant (accession “6-12-6”) plants. The efficiency of transfection was verified using a β-glucuronidase (GUS) reporter and was found to comprise most leaf areas regardless of the initial leaf position. Upon infection with E. necator, clear differences were observed with respect to hyphal development between agro-infiltrated leaves and control groups of both, the susceptible and the resistant, genotypes. The expression of VpGLOX was followed by real-time polymerase chain reaction in both genotypes. Whereas in the susceptible host (“6-12-2”) expression was found to increase only in transfected leaves and remained transient, in the resistant host (“6-12-6”), a second peak appeared later in transfected leaves, probably representing the response of the endogenous VpGLOX. The data support the interpretation that VpGLOX is sufficient to confer resistance to E. necator.

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Acknowledgments

We are grateful to Dr. Elspeth Steel (The Food and Environment Research Agency Fera, Sand Hutton, York YO41 1LZ, UK) for critical reading of the manuscript and Huie Li, Yanyan Zhang, and Weirong Xu of Northwest A&F University for helpful advice. This work was supported by the National Science and Technology Support Program of China (Program for the Eleventh Five-Year Plan; Grant No. 2008BAD92B07), the National High Technology Research and Development Program of China (863 Program; Grant No. 2007AA10Z182), the National Natural Science Foundation of the People’s Republic of China (No. 30571280 and No. 30771493), and the earmarked fund for Modern Agro-industry Technology Research System from the Ministry of Agriculture of the People’s Republic of China (Grant No. Nycytx-30-zp-06).

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Authors and Affiliations

  1. College of Horticulture, Northwest A&F University, Yangling, Shaanxi, 712100, People’s Republic of China

    Xin Guan, Heqing Zhao, Yan Xu & Yuejin Wang

  2. Key Laboratory of Horticultural Plant Germplasm Resource Utilization in Northwest China, Ministry of Agriculture of the People’s Republic of China, Northwest A&F University, Yangling, Shaanxi, 712100, People’s Republic of China

    Xin Guan, Heqing Zhao, Yan Xu & Yuejin Wang

  3. Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling, Shaanxi, 712100, People’s Republic of China

    Xin Guan, Heqing Zhao, Yan Xu & Yuejin Wang

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  1. Xin Guan
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  2. Heqing Zhao
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  3. Yan Xu
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  4. Yuejin Wang
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Correspondence to Yuejin Wang.

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Handling Editor: Hanns H. Kassemeyer

Xin Guan and Heqing Zhao contributed equally to this work.

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Guan, X., Zhao, H., Xu, Y. et al. Transient expression of glyoxal oxidase from the Chinese wild grape Vitis pseudoreticulata can suppress powdery mildew in a susceptible genotype. Protoplasma 248, 415–423 (2011). https://doi.org/10.1007/s00709-010-0162-4

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