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The effects of cell recycling on the production of 1,3-propanediol by Klebsiella pneumoniae

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Abstract

The effects of both biomass age and cell recycling on the 1,3-propanediol (1,3-PDO) production by Klebsiella pneumoniae were investigated in a membrane-supported bioreactor using hollow-fiber ultrafiltration membrane module in two separate experiments. It was determined that older cells have a negative effect on 1,3-PDO production. The concentrations of by-products, such as acetic acid and ethanol, increased in cultures with older cells, whereas the concentrations of succinic acid, lactic acid and 2,3-butanediol decreased. The effect of cell recycling was comparatively studied at a cell recycling ratio of 100 %. The results showed that cell recycling had also negative effects on 1,3-PDO fermentation. It was hypothesized that both cell recycling and biomass age caused metabolic shifts to undesired by-products which then inhibited the 1,3-PDO production. On the other hand, the use of hollow-fiber ultrafiltration membrane module was found to be very effective in terms of removal of cells from the fermentation broth.

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Authors and Affiliations

  1. Bioengineering Department, Faculty of Engineering, Ege University, Bornova, 35100, Izmir, Turkey

    Fatma Gizem Avci, Damla Huccetogullari & Nuri Azbar

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  1. Fatma Gizem Avci
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  2. Damla Huccetogullari
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  3. Nuri Azbar
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Correspondence to Nuri Azbar.

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Avci, F.G., Huccetogullari, D. & Azbar, N. The effects of cell recycling on the production of 1,3-propanediol by Klebsiella pneumoniae . Bioprocess Biosyst Eng 37, 513–519 (2014). https://doi.org/10.1007/s00449-013-1018-z

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