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Signaling molecules and cell death in Melissa officinalis plants exposed to ozone

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Abstract

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The study focuses on the interaction between reactive oxygen species and hormones that regulate the programmed cell death in plants of Melissa officinalis exposed to ozone.

Abstract

Interaction between hormone and redox signaling pathways has been investigated in ozone-stressed (200 ppb, 5 h) lemon balm to verify if the response resembles the biotic defense reactions. In comparison to controls, plants exhibited foliar injury and the cell death was induced by (1) biphasic production of hydrogen peroxide and superoxide radical; (2) hormonal regulation of ozone-induced lesion formation with a significant production of ethylene, salicylic, jasmonic and abscisic acid; (3) ozone degradation to reactive oxygen species and their detoxification by some enzymatic (such as superoxide dismutase) and non-enzymatic antioxidant systems (such as ascorbic acid, glutathione and carotenoids), that worked in cooperation without providing a defense against free radicals (such as confirmed by the modification of the antioxidant properties of leaf tissue). This integrated view showed that reactive oxygen species interact with hormonal signaling pathway regulating cell death and the sensitivity of lemon balm to ozone.

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Acknowledgments

We gratefully acknowledge Dr. Giulia Fabiani and Dr. María José Díaz for their support during the biochemical analyses.

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Authors and Affiliations

  1. Department of Agriculture, Food and Environment, University of Pisa, Via del Borghetto 80, 56124, Pisa, Italy

    Elisa Pellegrini, Alice Trivellini, Alessandra Campanella, Alessandra Francini, Giacomo Lorenzini, Cristina Nali & Paolo Vernieri

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  1. Elisa Pellegrini
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  2. Alice Trivellini
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  3. Alessandra Campanella
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  4. Alessandra Francini
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  5. Giacomo Lorenzini
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  7. Paolo Vernieri
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Correspondence to Cristina Nali.

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Communicated by A. Feher.

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Pellegrini, E., Trivellini, A., Campanella, A. et al. Signaling molecules and cell death in Melissa officinalis plants exposed to ozone. Plant Cell Rep 32, 1965–1980 (2013). https://doi.org/10.1007/s00299-013-1508-0

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