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A genetic map of Lophopyrum ponticum chromosome 7E, harboring resistance genes to Fusarium head blight and leaf rust

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Abstract

The leaf rust resistance gene Lr19 and Fusarium head blight (FHB) resistance quantitative trait loci (QTL) derived from the wild wheatgrass Lophopyrum ponticum have been located on chromosome 7E. The main objectives of the present study were to develop a genetic map of chromosome 7E and map the two resistance loci using a population of 237 F7:8 recombinant inbred lines (RILs) derived from a cross between two Thatcher-L. ponticum substitution lines, K11463 (7el1(7D)) and K2620 (7el2(7D)). 532 G-SSR, E-SSR and STS markers from wheat chromosome group 7 were screened in the parent lines. Of these, 118 markers were polymorphic, with a polymorphism frequency of 22.2%. A genetic map of L. ponticum chromosome 7E was constructed with 64 markers, covering 95.76 cM, with an average genetic distance of 1.47 cM between markers. The major FHB resistance locus, temporarily assigned as FhbLoP, was mapped to the very distal region of the long arm of chromosome 7E within a 3.71 cM interval flanked by Xcfa2240 and Xswes19, which accounts for 30.46% of the phenotypic variance. Lr19 was bracketed by Xwmc273 and XBE404744, with a map distance of 1.54 and 1.43 cM from either side, respectively. The closely linked markers identified in this study will be helpful for marker-assisted introgression of the L. ponticum-derived FhbLoP and Lr19 genes into elite cultivars of wheat, and the development of a genetic map will accelerate the map-based cloning of these two genes.

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Acknowledgments

The authors acknowledge financial support by the National Basic Research Program of China (973) (Grant No. 2009CB118301), Transgenic Special Item (Grants No. 2008ZX08002-004 and No. 2008ZX08009-003), NSF of China (Grant No. 30871514 and No. 31071405), NSF of Shandong (Grant No. Y2008D31), and USDA-ARS USWBSI (Grant No. 5902069081). We are also grateful to Dr. G. Shaner, Department of Botany and Plant Pathology, Purdue University and to Dr. Xiu’e Wang, Department of Agronomy, Nanjing Agricultural University, for providing isolates of F. graminearum, and to Drs. W. Chen and T. Liu, State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, for providing the Puccinia triticina isolate 09-10-2 (syn. 121 R63-1).

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Authors and Affiliations

  1. State Key Laboratory of Crop Biology, Shandong Agricultural University, 61 Daizong Avenue, Taian, 271018, China

    Xiuli Zhang, Yuanfeng Hao, Jinjin Cai & Lingrang Kong

  2. Department of Agronomy, Purdue University, 915 W. State St., West Lafayette, IN, 47907, USA

    Xiaorong Shen & Herbert W. Ohm

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  1. Xiuli Zhang
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  2. Xiaorong Shen
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  5. Herbert W. Ohm
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  6. Lingrang Kong
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Correspondence to Lingrang Kong.

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Communicated by P. Langridge.

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Zhang, X., Shen, X., Hao, Y. et al. A genetic map of Lophopyrum ponticum chromosome 7E, harboring resistance genes to Fusarium head blight and leaf rust. Theor Appl Genet 122, 263–270 (2011). https://doi.org/10.1007/s00122-010-1441-3

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  • DOI: https://doi.org/10.1007/s00122-010-1441-3

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