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Mutation and Mutation Screening

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Cereal Genomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1099))

Abstract

Molecular techniques have created the opportunity for great advances in plant mutation genetics and the science of mutation breeding. The powerful targeted induced local lesions in genomes (TILLING) technique has introduced the possibility of reverse genetics—the ability to screen for mutations at the DNA level prior to assessing phenotype. Fundamental to TILLING is the induction of mutant populations (or alternatively, the identification of mutants in the environment); and mutation induction requires an understanding and assessment of the appropriate mutagen dose required. The techniques of mutation induction, dose optimization, and TILLING are explained.

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Acknowledgments

Authors B.J.T., O.A.H., and J.J-C. wish to thank Kamila Kozak-Stankiewicz for supplying lupine samples used for making Fig. 2c. Funding for the work on low-cost TILLING and EcoTILLING was provided by the Food and Agriculture Organization of the United Nations and the International Atomic Energy Agency through their Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture.

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Authors and Affiliations

  1. Cooperative Research Centre for Remote Economic Participation, Division of Research, Southern Cross University, Lismore, NSW, Australia

    L. Slade Lee

  2. Plant Breeding and Genetics Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, IAEA Laboratories Seibersdorf, Vienna, Austria

    Bradley J. Till, Owen A. Huynh & Joanna Jankowicz-Cieslak

  3. Southern Cross Plant Science, Southern Cross University, Lismore, NSW, Austria

    Helen Hill

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  1. L. Slade Lee
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  2. Bradley J. Till
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  3. Helen Hill
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  4. Owen A. Huynh
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  5. Joanna Jankowicz-Cieslak
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Editors and Affiliations

  1. Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St. Lucia, Queensland, Australia

    Robert J. Henry

  2. Centre for Nutrition and Food Science, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Lismore, New South Wales, Australia

    Agnelo Furtado

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Lee, L.S., Till, B.J., Hill, H., Huynh, O.A., Jankowicz-Cieslak, J. (2014). Mutation and Mutation Screening. In: Henry, R., Furtado, A. (eds) Cereal Genomics. Methods in Molecular Biology, vol 1099. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-715-0_8

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  • DOI: https://doi.org/10.1007/978-1-62703-715-0_8

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-714-3

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