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TILLING moves beyond functional genomics into crop improvement

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Abstract

Transgenic methods have been successfully applied to trait improvement in a number of crops. However, reverse genetics studies by transgenic means are not practical in many commercially important crops, hampering investigations into gene function and the development of novel and improved cultivars. A nontransgenic method for reverse genetics called Targeting Induced Local Lesions IN Genomes (TILLING) has been developed as a method for inducing and identifying novel genetic variation, and has been demonstrated in the model plant, Arabidopsis thaliana. Recently, TILLING has been extended to the improvement of crop plants and shows great promise as a general method for both functional genomics and modulation of key traits in diverse crops.

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References

  • T Colbert BJ Till R Tompa S Reynolds MN Steine AT Yeung CM McCallum L Comai S Henikoff (2001) ArticleTitleHigh-throughput screening for induced point mutations Plant Physiol 126 480–484 Occurrence Handle10.1104/pp.126.2.480 Occurrence Handle1:CAS:528:DC%2BD3MXks1Gnurc%3D Occurrence Handle11402178

    Article  CAS  PubMed  Google Scholar 

  • L Comai K Young BJ Till SH Reynolds EA Greene CA Codomo LC Enns JE Johnson C Burtner AR Odden S Henikoff (2004) ArticleTitleEfficient discovery of DNA polymorphisms in natural populations by Ecotilling Plant J 37 778–786 Occurrence Handle10.1111/j.0960-7412.2003.01999.x Occurrence Handle1:CAS:528:DC%2BD2cXislyltLo%3D Occurrence Handle14871304

    Article  CAS  PubMed  Google Scholar 

  • P Gepts R Papa (2002) ArticleTitleEvolution during domestication Encycl Life Sci: 1 7

    Google Scholar 

  • RA Graybosch (1998) ArticleTitleWaxy wheats: Origin, properties, and prospects Trends in Food Science and Technology 9 135–142 Occurrence Handle10.1016/S0924-2244(98)00034-X Occurrence Handle1:CAS:528:DyaK1cXltVOksro%3D

    Article  CAS  Google Scholar 

  • S Henikoff BJ Till L Comai (2004) ArticleTitleTILLING. Traditional Mutagenesis Meets Functional Genomics Plant Physiol 135 630–636 Occurrence Handle10.1104/pp.104.041061 Occurrence Handle1:CAS:528:DC%2BD2cXltlKjtb0%3D Occurrence Handle15155876

    Article  CAS  PubMed  Google Scholar 

  • GS Khush (2001) ArticleTitleGreen Revolution: The way forward Nature Rev Genet 2 815–822 Occurrence Handle10.1038/35093585 Occurrence Handle1:CAS:528:DC%2BD38Xmt1Wgu7w%3D

    Article  CAS  Google Scholar 

  • A Kodym R Afza (2003) ArticleTitlePhysical and chemical mutagenesis Methods Mol Biol 236 189–204 Occurrence Handle1:CAS:528:DC%2BD3sXnsVSgtr8%3D Occurrence Handle14501066

    CAS  PubMed  Google Scholar 

  • D Kuhn J Borrone A Meerow J Motamayor J Brown R Schnell (2005) ArticleTitleSingle-strand conformation polymorphism analysis of candidate genes for reliable identification of alleles by capillary array electrophoresis Electrophoresis 26 112–125 Occurrence Handle1:CAS:528:DC%2BD2MXpsFGmug%3D%3D Occurrence Handle15624191

    CAS  PubMed  Google Scholar 

  • P-Y Kwok (2001) ArticleTitleMethods for genotyping single nucleotide polymorphisms Annu Rev Genomics Hum Genet 2 235–258 Occurrence Handle1:CAS:528:DC%2BD3MXos1antbg%3D Occurrence Handle11701650

    CAS  PubMed  Google Scholar 

  • RJ Lawrence CS Pikaard (2003) ArticleTitleTransgene-induced RNA interference: a strategy for overcoming gene redundancy in polyploids to generate loss-of-function mutations Plant J 36 114–121 Occurrence Handle1:CAS:528:DC%2BD3sXos12mt7s%3D Occurrence Handle12974816

    CAS  PubMed  Google Scholar 

  • Q Li Z Liu H Monroe CT Culiat (2002) ArticleTitleIntegrated platform for detection of DNA sequence variants using capillary array electrophoresis Electrophoresis 23 1499–4511 Occurrence Handle10.1002/1522-2683(200205)23:10<1499::AID-ELPS1499>3.0.CO;2-X Occurrence Handle1:CAS:528:DC%2BD38XksVWkurk%3D Occurrence Handle12116161

    Article  CAS  PubMed  Google Scholar 

  • SL Lilleberg (2003) ArticleTitleIn-depth mutation and SNP discovery using DHPLC gene scanning Curr Opin Drug Discov Devel 6 237–252 Occurrence Handle1:CAS:528:DC%2BD3sXislOqsLc%3D Occurrence Handle12669460

    CAS  PubMed  Google Scholar 

  • CA Oleykowski CR Bronson Mullins AK Godwin AT Yeung (1998) ArticleTitleMutation detection using a novel plant endonuclease Nucleic Acids Res 26 4597–4602 Occurrence Handle1:CAS:528:DyaK1cXnt1Oks7Y%3D Occurrence Handle9753726

    CAS  PubMed  Google Scholar 

  • MB Silvarolla P Mazzafera LC Fazuoli (2004) ArticleTitleA naturally decaffeinated arabica coffee Nature 429 826 Occurrence Handle1:CAS:528:DC%2BD2cXltVKlt7o%3D Occurrence Handle15215853

    CAS  PubMed  Google Scholar 

  • AJ Slade SI Fuerstenberg D Loeffler MN Steine D Facciotti (2005) ArticleTitleA reverse genetic, nontransgenic approach to wheat crop improvement by TILLING Nat Biotechnol 23 75–81 Occurrence Handle1:CAS:528:DC%2BD2MXhsFGntg%3D%3D Occurrence Handle15580263

    CAS  PubMed  Google Scholar 

  • P Stanssens M Zabeau G Meersseman G Rernes Y Gansemans N Storm R Hartmer C Honisch CP Rodi S Bocker Dvd Boom (2004) ArticleTitleHigh-throughput MALDI-TOF discovery of genomic sequence polymorphisms Genome Res 14 126–133 Occurrence Handle1:CAS:528:DC%2BD2cXlvVGmug%3D%3D Occurrence Handle14707174

    CAS  PubMed  Google Scholar 

  • BJ Till SH Reynolds EA Greene CA Codomo LC Enns JE Johnson C Burtner AR Odden K Young NE Taylor JG Henikoff L Comai S Henikoff (2003) ArticleTitleLarge-scale discovery of induced point mutations with high-throughput TILLING Gen Res 13 524–530 Occurrence Handle1:CAS:528:DC%2BD3sXit1WgtLs%3D

    CAS  Google Scholar 

  • D Zamir (2001) ArticleTitleImproving plant breeding with exotic genetic libraries Nature Rev Genet 2 983–989 Occurrence Handle1:CAS:528:DC%2BD38Xmt1WgsrY%3D

    CAS  Google Scholar 

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Authors and Affiliations

  1. Anawah Inc., 1102 Columbia Street, Suite 600, Seattle, WA, 98104, USA

    Ann J. Slade & Vic C. Knauf

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  1. Ann J. Slade
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  2. Vic C. Knauf
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Slade, A.J., Knauf, V.C. TILLING moves beyond functional genomics into crop improvement. Transgenic Res 14, 109–115 (2005). https://doi.org/10.1007/s11248-005-2770-x

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  • DOI: https://doi.org/10.1007/s11248-005-2770-x

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