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Analysis of Volatile Mouse Pheromones by Gas Chromatography Mass Spectrometry

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Pheromone Signaling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1068))

Abstract

High-precision quantitative profiling of volatile organic constituents in rodent physiological fluids and glandular secretions is needed to relate olfactory signals to physiology and behavior. Whereas capillary gas chromatography-mass spectrometry (GC-MS) analysis has become the most widely applied in such investigations, the extraction and preconcentration of volatile organics is arguably the most critical step in the overall analytical task. In this chapter, we describe technical details of two main sample extraction procedures used in our laboratory: dynamic headspace trapping, and stir bar sorptive extraction (SBSE). They have been demonstrated here for the chromatographic analysis of mouse urine, serum, saliva, and preputial gland specimens.

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Acknowledgments

This work was jointly sponsored by the METACyt Initiative of Indiana University, a major grant from the Lilly Endowment, Inc., and the Lilly Chemistry Alumni Chair funds (to M.V.N.).

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Authors and Affiliations

  1. Department of Chemistry, Institute for Pheromone Research, Indiana University, Bloomington, IN, USA

    Milos V. Novotny & Helena A. Soini

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  1. Milos V. Novotny
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  2. Helena A. Soini
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Editors and Affiliations

  1. Dept. of Applied Biological Chemistry, The University of Tokyo School of Agricultural & Life Sciences, Tokyo, Japan

    Kazushige Touhara

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Novotny, M.V., Soini, H.A. (2013). Analysis of Volatile Mouse Pheromones by Gas Chromatography Mass Spectrometry. In: Touhara, K. (eds) Pheromone Signaling. Methods in Molecular Biology, vol 1068. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-619-1_3

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  • DOI: https://doi.org/10.1007/978-1-62703-619-1_3

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  • Publisher Name: Humana Press, Totowa, NJ

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