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Cellular and Morpho-histological Foundations of In Vitro Plant Regeneration

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Plant Cell Culture Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1815))

Abstract

In vitro plant regeneration systems have turned into invaluable tools to plant biotechnology. Despite being poorly understood, the molecular mechanisms underlying the control of both morphogenetic pathways, de novo organogenesis and somatic embryogenesis, have been supported by recent findings involving proteome-, metabolome-, and transcriptome-based profiles. Notwithstanding, the integration of molecular data with structural aspects has been an important strategy of study attempting to elucidate the basis of the cell competence acquisition to further follow commitment and determination to specific a particular in vitro regeneration pathway. In that sense, morpho-histological tools have allowed to recognize cellular markers and patterns of gene expression at cellular level and this way have collaborated in the identification of the cell types with high regenerative capacity. This chapter ties together up those fundamental and important microscopy techniques that help to elucidate that regeneration occurs, most of the time, from epidermis or subepidermal cells and from the procambial cells (pericycle and vascular parenchyma). Important findings are discussed toward ultrastructural differences observed in the nuclear organization among pluripotent and totipotent cells, implying that regeneration occurs from two cellular mechanisms based on cellular reprogramming or reactivation.

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Acknowledgments

This work was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brasília, DF, Brazil), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Brasília, DF, Brazil), and Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG, Belo Horizonte, MG, Brazil). Authors are grateful to the following colleagues who generously made available some figures used in this chapter: Dr. Wellington M. Barbosa (Fig. 1b), Dr. Maria Yumbla-Orbes (Figs. 1h and 4g–i), Dr. Elyabe M. Matos (Fig.4e, f), and MSc Ludmila N. F. Correia (Fig. 1g). The Núcleo de Apoio à Pesquisa em Microscopia Eletrônica Aplicada à Agricultura (NAP/MEPA-ESALQ/USP, Piracicaba, SP, Brazil) and Núcleo de Microscopia e Microanálises (NMM/UFV ,Viçosa, MG, Brazil) are also acknowledged.

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Authors and Affiliations

  1. Instituto de Biociências, Universidade Federal de Goiás, Jataí, GO, Brazil

    Diego Ismael Rocha

  2. Laboratório de Cultura de Tecidos—LCT, Instituto de Biotecnologia Aplicada à Agropecuária-BIOAGRO/Departamento de Biologia Vegetal, Campus Universitário, Universidade Federal de Viçosa, Viçosa, MG, Brazil

    Lorena Melo Vieira, Andréa Dias Koehler & Wagner Campos Otoni

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  1. Diego Ismael Rocha
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  2. Lorena Melo Vieira
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  3. Andréa Dias Koehler
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  4. Wagner Campos Otoni
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Correspondence to Wagner Campos Otoni .

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Editors and Affiliations

  1. Centro de Investigación Científica de Yucatán, Unidad de Bioquímica y Biología, Molecular de Plantas, Mérida, Yucatán, Mexico

    Víctor M. Loyola-Vargas

  2. Departamento de Ingeniería Genética, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Irapuato, Guanajuato, Mexico

    Neftalí Ochoa-Alejo

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Rocha, D.I., Vieira, L.M., Koehler, A.D., Otoni, W.C. (2018). Cellular and Morpho-histological Foundations of In Vitro Plant Regeneration. In: Loyola-Vargas, V., Ochoa-Alejo, N. (eds) Plant Cell Culture Protocols. Methods in Molecular Biology, vol 1815. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8594-4_3

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  • DOI: https://doi.org/10.1007/978-1-4939-8594-4_3

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