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Plant Cell Culture Protocols

  • Víctor M. Loyola-Vargas
  • Neftalí Ochoa-Alejo

Part of the Methods in Molecular Biology book series (MIMB, volume 1815)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Victor M. Loyola-Vargas, Neftalí Ochoa-Alejo
      Pages 3-13
  3. Cell Culture the Fundaments

    1. Front Matter
      Pages 15-15
    2. Jean Carlos Cardoso, Lee Tseng Sheng Gerald, Jaime A. Teixeira da Silva
      Pages 17-46
    3. Diego Ismael Rocha, Lorena Melo Vieira, Andréa Dias Koehler, Wagner Campos Otoni
      Pages 47-68
    4. Victor Gaba, Yehudit Tam, Danny Shavit, Benjamin Steinitz
      Pages 89-101
    5. Víctor M. Loyola-Vargas, Randy N. Avilez-Montalvo
      Pages 131-148
  4. Protocols

    1. Front Matter
      Pages 149-149
    2. Benjamín Rodríguez-Garay, José Manuel Rodríguez-Domínguez
      Pages 151-159
    3. Luis Sáenz, José Luis Chan, María Narvaez, Carlos Oropeza
      Pages 161-170
    4. Yessica López-Ramírez, Alejandra Palomeque-Carlín, Lucía Isabel Chávez Ortiz, Ma. de Lourdes de la Rosa-Carrillo, Eugenio Pérez-Molphe-Balch
      Pages 171-177
    5. Ruth E. Márquez-López, Ángela Ku-González, Hugo A. Méndez-Hernández, Rosa M. Galaz-Ávalos, Víctor M. Loyola-Vargas
      Pages 179-188
    6. José Luis Cabrera-Ponce, Itzel Anayetzi González-Gómez, Claudia G. León-Ramírez, José A. Sánchez-Arreguín, Alba E. Jofre y Garfias
      Pages 189-206
    7. Rosa M. Galaz-Ávalos, Heydi G. Martínez-Sánchez, Víctor M. Loyola-Vargas
      Pages 207-214
    8. Rosa Maria Escobedo-Gracia-Medrano, Carlos Iván Cruz-Cárdenas, Lucila Aurelia Sánchez-Cach, José Roberto Ku-Cauich, Wilma Aracely González-Kantún
      Pages 215-226
    9. Claudia Garcia, Jean-Philippe Marelli, Juan Carlos Motamayor, Cristiano Villela
      Pages 227-245
    10. Pilar S. Testillano, Aránzazu Gómez-Garay, Beatriz Pintos, María C. Risueño
      Pages 247-256
    11. Min-Rui Wang, Long Chen, Zhibo Zhang, Dag-Ragnar Blystad, Qiao-Chun Wang
      Pages 257-268
    12. Fernanda Vidigal Duarte Souza, Everton Hilo de Souza, Ergun Kaya, Lívia de Jesus Vieira, Ronilze Leite da Silva
      Pages 269-277
    13. Fernanda Vidigal Duarte Souza, Everton Hilo de Souza, Ronilze Leite da Silva
      Pages 279-288
    14. Benjamín Rodríguez-Garay, Sigifredo López-Díaz, José Manuel Rodríguez-Domínguez, Antonia Gutiérrez-Mora, Ernesto Tapia-Campos
      Pages 289-300
    15. Rosa María Escobedo-Gracia-Medrano, Martha Josefa Burgos-Tan, José Roberto Ku-Cauich, Adriana Quiroz-Moreno
      Pages 317-332
    16. Antonio Bernabé-Antonio, Amalia Maldonado-Magaña, María Elena Estrada-Zúñiga, Leticia Buendía-González, Francisco Cruz-Sosa
      Pages 333-337
    17. André Luis Wendt dos Santos, Ricardo Souza Reis, Angelo Schuabb Heringer, Eny Iochevet Segal Floh, Claudete Santa-Catarina, Vanildo Silveira
      Pages 339-349
    18. Brenda Anabel López-Ruiz, Vasti Thamara Juárez-González, Elva Carolina Chávez-Hernández, Tzvetanka D. Dinkova
      Pages 397-410
    19. Elsa Góngora-Castillo, Geovanny I. Nic-Can, Rosa M. Galaz-Ávalos, Víctor M. Loyola-Vargas
      Pages 411-427
    20. Felipe A. Vázquez-Flota, María de Lourdes Miranda-Ham
      Pages 429-435
    21. Andrey S. Marchev, Zhenya P. Yordanova, Milen I. Georgiev
      Pages 457-474
    22. Yeseña Burgos-May, Elidé Avilés-Berzunza, Luis Manuel Peña-Rodríguez, Gregorio Godoy-Hernández
      Pages 475-492
  5. Back Matter
    Pages 493-507

About this book

Introduction

This fourth edition provides new and updated protocols on plant cell, tissue, and organ cultures. Chapters are divided into five parts that cover topics from general methodologies, statistical analysis and contamination control, highly specialized techniques, and laborious process of measuring the epigenetics changes in tissue cultures. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

  Authoritative and cutting-edge, Plant Cell Culture Protocols, Fourth Edition aims to serve both professionals and novices with its guide to the most common and applicable techniques and methods for plant tissue and cell culture.

Keywords

Aseptic culture antibiotics Cryopreservation Haploids Epigenetics

Editors and affiliations

  • Víctor M. Loyola-Vargas
    • 1
  • Neftalí Ochoa-Alejo
    • 2
  1. 1.Centro de Investigación Científica de YucatánUnidad de Bioquímica y Biología, Molecular de PlantasMéridaMexico
  2. 2.Departamento de Ingeniería GenéticaUnidad Irapuato, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico NacionalIrapuatoMexico

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-8594-4
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8593-7
  • Online ISBN 978-1-4939-8594-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site