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Isolation of Environmental Bacteria Able to Degrade Sterols and/or Bile Acids: Determination of Cholesterol Oxidase and Several Hydroxysteroid Dehydrogenase Activities in Rhodococcus, Gordonia, and Pseudomonas putida

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Microbial Steroids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2704))

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Abstract

Interest about the isolation and characterization of steroid-catabolizing bacteria has increased over time due to the massive release of these recalcitrant compounds and their deleterious effects or their biotransformation derivatives as endocrine disruptors for wildlife, as well as their potential use in biotechnological approaches for the synthesis of pharmacological compounds. Thus, in this chapter, an isolation protocol to select environmental bacteria able to degrade sterols, bile acids, and androgens is shown. Moreover, procedures for the determination of cholesterol oxidase or different hydroxysteroid dehydrogenase activities in Pseudomonas putida DOC21, Rhodococcus sp. HE24.12, Gordonia sp. HE24.4J and Gordonia sp. HE24.3 are also detailed.

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Acknowledgments

Development of the methods here presented has been supported by the Ministerio de Economía y Competitividad (Madrid, España, grants BFU2009-11545-C03-01, BIO2012-39695-C02-02, and BIO2015-66960-C3-3R), by a CENIT Project RTC-2014-2249-1 (CDTI, Ministerio de Economía y Competitividad, Madrid, España), and by a grant from the Junta de Castilla y León (Consejería de Educación, Valladolid, España) LE246A11-2. The authors also want to thank the support to their actual research by the Horizon Europe Framework Programme (call: HORIZON-CL4-2021-RESILIENCE-01-11) through the ESTELLA project (“DESign of bio-based Thermoset polymer with rEcycLing capabiLity by dynAmic bonds for bio-composite manufacturing”) (Project no. 101058371), the Ministerio de Ciencia e Innovación (grant TED2021-132593B-I00 belonging to the 2021 convocatory “Proyectos Estratégicos Orientados a la Transición Ecológica y a la Transición Digital” and RTI2018-095584-B-C43 from Proyectos de I+D+i RETOS INVESTIGACION), and the Junta de Castilla y León, grant LE250P20.

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Authors and Affiliations

  1. Área de Bioquímica y Biología Molecular, Departamento de Biología Molecular, Facultad de Veterinaria, Universidad de León, León, Spain

    Alejandro Chamizo-Ampudia, Luis Getino, José M. Luengo & Elias R. Olivera

Authors
  1. Alejandro Chamizo-Ampudia
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  2. Luis Getino
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  3. José M. Luengo
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  4. Elias R. Olivera
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Corresponding author

Correspondence to Alejandro Chamizo-Ampudia .

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Editors and Affiliations

  1. Área de Bioquímica y Biología Molecular, Departamento de Biología Molecular, Facultad de Veterinaria, Universidad de León, León, Spain

    Carlos Barreiro

  2. Department of Biotechnology, Curia Spain, León, Spain

    José-Luis Barredo

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Chamizo-Ampudia, A., Getino, L., Luengo, J.M., Olivera, E.R. (2023). Isolation of Environmental Bacteria Able to Degrade Sterols and/or Bile Acids: Determination of Cholesterol Oxidase and Several Hydroxysteroid Dehydrogenase Activities in Rhodococcus, Gordonia, and Pseudomonas putida. In: Barreiro, C., Barredo, JL. (eds) Microbial Steroids. Methods in Molecular Biology, vol 2704. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3385-4_2

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  • DOI: https://doi.org/10.1007/978-1-0716-3385-4_2

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3384-7

  • Online ISBN: 978-1-0716-3385-4

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