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Microbial Steroids

Methods and Protocols

  • Book
  • © 2023
  • Latest edition

Overview

Editors:
  1. Carlos Barreiro

    1. Área de Bioquímica y Biología Molecular, Departamento de Biología Molecular, Facultad de Veterinaria, Universidad de León, León, Spain

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    You can also search for this editor in PubMed   Google Scholar

  2. José-Luis Barredo

    1. Department of Biotechnology, Curia Spain, León, Spain

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2704)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. General Overview

    1. Front Matter

      Pages 1-1
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    2. Current Trends and Perspectives in Microbial Bioconversions of Steroids

      • Marina V. Donova
      Pages 3-21

  3. Microbial Screening and Genetic Manipulation

    1. Front Matter

      Pages 23-23
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    2. Isolation of Environmental Bacteria Able to Degrade Sterols and/or Bile Acids: Determination of Cholesterol Oxidase and Several Hydroxysteroid Dehydrogenase Activities in Rhodococcus, Gordonia, and Pseudomonas putida

      • Alejandro Chamizo-Ampudia, Luis Getino, José M. Luengo, Elias R. Olivera
      Pages 25-42

    3. Selection of Biodegrading Phytosterol Strains

      • María-Angélica Mondaca, Maricel Vidal, Soledad Chamorro, Gladys Vidal
      Pages 43-50

    4. Identification and Characterization of Some Genes, Enzymes, and Metabolic Intermediates Belonging to the Bile Acid Aerobic Catabolic Pathway from Pseudomonas

      • José M. Luengo, Elias R. Olivera
      Pages 51-83

    5. Targeted Mutagenesis of Mycobacterium Strains by Homologous Recombination

      • Shikui Song, Zhengding Su
      Pages 85-96

  4. Genetic and Biochemical Analyses

    1. Front Matter

      Pages 97-97
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    2. RNA Preparation and RNA-Seq Bioinformatics for Comparative Transcriptomics

      • Antonio Rodríguez-García, Alberto Sola-Landa, Carlos Barreiro
      Pages 99-113

    3. Bidimensional Analyses of the Intra- and Extracellular Proteomes of Steroid Producer Mycobacteria

      • Carlos Barreiro, Ana M. Ibáñez
      Pages 115-141

    4. Fungal Sterol Analyses by Gas Chromatography–Mass Spectrometry Using Different Derivatives

      • Aurélie Mossion, Isabelle Ourliac-Garnier, Gaetane Wielgosz-Collin
      Pages 143-156

    5. Cholesterol Assay Based on Recombinant Cholesterol Oxidase, ABTS, and Horseradish Peroxidase

      • Alexey V. Sviridov, Mikhail V. Karpov, Victoria V. Fokina, Marina V. Donova
      Pages 157-171

    6. Measuring the Interaction of Sterols and Steroids with Proteins by Microscale Thermophoresis

      • Ola El Atab, Roger Schneiter
      Pages 173-181

  5. Fermentation and Biocatalysis for Steroids Production

    1. Front Matter

      Pages 183-183
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    2. Cultivation of Mycolicibacterium spp. Mutants in Miniaturized and High-Throughput Format to Characterize Their Growth, Phytosterol Conversion Ability, and Resistance to the Steroid Products

      • Simone Balzer Le, Anna Nordborg, Kjell Domaas Josefsen, Silje Malene Olsen, Håvard Sletta
      Pages 185-200

    3. β-Sitosterol Bioconversion in Small-Scale Devices: From Microtiter Plates to Microfluidic Reactors

      • Marco P. C. Marques, Jorge Aranda Hernandez, Pedro Fernandes
      Pages 201-219

    4. Biocatalysis of Steroids by Mycobacterium sp. in Aqueous and Organic Media

      • Carla C. C. R. de Carvalho, Pedro Fernandes
      Pages 221-229

    5. Scale-Up of Phytosterols Bioconversion into Androstenedione

      • Sonia Martínez-Cámara, Manuel de la Torre, José-Luis Barredo, Marta Rodríguez-Sáiz
      Pages 231-243

    6. Bioconversion of Phytosterols into Androstenedione by Mycolicibacterium

      • Kjell Domaas Josefsen, Anna Nordborg, Simone Balzer Le, Silje Malene Olsen, Håvard Sletta
      Pages 245-267
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Keywords

About this book

This detailed volume explores experimental laboratory procedures for a wide range of steroid bioconversions. After an overview on the current trends and perspectives, the book continues with sections covering microbial screening and synthetic biology applied to microorganisms able to catabolize sterols, methods on strain characterization, including omics and biochemical analyses, methods of fermentation and biocatalysis for steroids production, as well as a chapter on the medical use of glucocorticoids in cancer patients. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Microbial Steroids: Methods and Protocols, Second Edition serves as an ideal reference source for laboratory and industrial professionals, as well as for students in a wide array of biological disciplines.

Editors and Affiliations

  • Área de Bioquímica y Biología Molecular, Departamento de Biología Molecular, Facultad de Veterinaria, Universidad de León, León, Spain

    Carlos Barreiro

  • Department of Biotechnology, Curia Spain, León, Spain

    José-Luis Barredo

Bibliographic Information

  • Book Title: Microbial Steroids

  • Book Subtitle: Methods and Protocols

  • Editors: Carlos Barreiro, José-Luis Barredo

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3385-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-3384-7Published: 30 August 2023

  • Softcover ISBN: 978-1-0716-3387-8Due: 30 September 2023

  • eBook ISBN: 978-1-0716-3385-4Published: 29 August 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIII, 355

  • Number of Illustrations: 25 b/w illustrations, 48 illustrations in colour

  • Topics: Biochemistry, general, Biotechnology, Biological Techniques

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