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Photosynthetic capacity, growth and water relations in ‘Golden’ papaya cultivated in vitro with modifications in light quality, sucrose concentration and ventilation

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Abstract

This study assessed the ecophysiological aspects of the in vitro multiplication of ‘Golden’ papaya in response to different light qualities, ventilation systems and sucrose concentrations related to photosynthesis, chlorophyll fluorescence, water balance and growth. The treatments were performed in a complete, randomised split-plot design with four replicates. These treatments consisted of white and red light, closed and ventilated culture systems and four sucrose concentrations (10, 20, 30 and 40 g L−1) in the culture medium. The lowest plantlet water loss rate was obtained using the ventilated culture system. The photochemical damage was attributable to the reduced maximum PS II quantum yield and the efficiency of the oxygen-evolving complex (OEC). The increase in the production of papaya dry biomass was due to the exogenous carbon source, the sucrose that was added to the culture medium. In this study, there was no photosynthetic carbon assimilation (Aactual, actual photosynthetic rate) or oxygen evolution (Apot, potential photosynthetic rate) due to the damage caused by the presence of sucrose in the culture medium to the photochemical capacity of the oxygen-evolving complex (OEC) and PS II activity. Further studies are needed using a sucrose concentration below 10 g L−1 in association with the increased irradiance of the culture chamber (red lights) as well as carbon dioxide enrichment (over 400 μL L−1 of air). These conditions may stimulate the autotrophic metabolism of this species for in vitro cultivation and increase the rate of biomass production.

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Acknowledgment

We acknowledge the support of the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and the Universidade Estadual do Norte Fluminense Darcy Ribeiro. The authors wish to thank Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brazil) for the fellowships awarded to E. Campostrini.

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Authors and Affiliations

  1. Laboratório de Melhoramento de Plantas, PPGAT, Universidade Federal do Espírito Santo, São Mateus, ES, Brazil

    Omar Schmildt & Edilson Romais Schmildt

  2. Setor de Fisiologia Vegetal, CCTA, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes, RJ, Brazil

    Alena Torres Netto & Eliemar Campostrini

  3. Laboratório de Fitotecnia, Cultura de Tecidos Vegetais, CCTA, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes, RJ, Brazil

    Virginia Silva Carvalho

  4. Departamento de Biologia Vegetal, Universidade Federal de Viçosa, Viçosa, MG, Brazil

    Wagner Campos Otoni

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  1. Omar Schmildt
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  2. Alena Torres Netto
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  3. Edilson Romais Schmildt
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  6. Eliemar Campostrini
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Correspondence to Eliemar Campostrini.

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Schmildt, O., Netto, A.T., Schmildt, E.R. et al. Photosynthetic capacity, growth and water relations in ‘Golden’ papaya cultivated in vitro with modifications in light quality, sucrose concentration and ventilation. Theor. Exp. Plant Physiol. 27, 7–18 (2015). https://doi.org/10.1007/s40626-014-0026-y

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