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Effects of different spectral lights on Oncidium PLBs induction, proliferation, and plant regeneration

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Abstract

The effects of different spectral light distribution on in vitro induction and proliferation of Oncidium protocorm-like bodies (PLBs) and subsequent growth of plantlets were investigated. Shoot tips (5 mm in length) of proliferating shoots of Oncidium “Gower Ramsey” were vertically incubated on 1/2 Murashige and Skoog (MS) medium supplemented with 1.0 mg l−1 6-benzyladenine (BA), and grown under either monochromatic red light-emitting diodes (LEDs) (RR), blue LEDs (BB), yellow LEDs (YY) or green LEDs (GG). Cultures grown under fluorescent lamps (FL) were used as control. Selected FL-induced PLBs were cut into 3- to 4-mm sections and incubated on MS medium supplemented with 1.0 mg l−1 BA and 0.5 mg l−1 α-naphthaleneacetic acid (NAA), and grown under RR, BB, YY, GG, or FL. Moreover, FL-differented shoots (15 mm in length with two leaves) were incubated on 1/2 MS medium with 0.5 mg l−1 NAA, and grown under either FL, RR, 10% blue + 90% red LEDs (1BR), 20% blue + 80% red LEDs (2BR), 30% blue + 70% red LEDs (3BR), BB, 80% red + 10% blue + 10% far-red LEDs (RBFr), or 80% red + 10% blue + 10% green LEDs (RBG). Overall, the red light spectrum enhanced induction, proliferation, and the carbohydrate contents of PLBs, as well as subsequent plantlet lengths, while the blue spectrum promoted differentiation, protein accumulation, and enzyme activities in PLBs, as well as pigment content accumulation in PLBs and developing plantlets. The combination of red and blue LEDs resulted in higher energy efficiency as well as dry weight and enzyme activities in these plantlets.

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Acknowledgments

This project was partially funded by National Science and Technology Support Project of China (No: 2010AA03A165) and Natural Science Fundation of China (No: 30972035).

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Authors and Affiliations

  1. College of Agronomy, Nanjing Agricultural University, 210095, Nanjing, People’s Republic of China

    Liu Mengxi, Xu Zhigang & Yang Yang

  2. Xuzhou Institute of Technology, 221008, Xuzhou, People’s Republic of China

    Feng Yijie

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  1. Liu Mengxi
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  2. Xu Zhigang
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  3. Yang Yang
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  4. Feng Yijie
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Correspondence to Xu Zhigang.

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Mengxi, L., Zhigang, X., Yang, Y. et al. Effects of different spectral lights on Oncidium PLBs induction, proliferation, and plant regeneration. Plant Cell Tiss Organ Cult 106, 1–10 (2011). https://doi.org/10.1007/s11240-010-9887-1

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  • DOI: https://doi.org/10.1007/s11240-010-9887-1

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