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In vitro leaf anatomy, ex vitro photosynthetic behaviors and growth of Calathea orbifolia (Linden) Kennedy plants obtained from semi-solid medium and temporary immersion systems

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Abstract

In vitro bud clusters of Calathea orbifolia (Linden) Kennedy were obtained and subcultured in semi-solid (agar) medium and temporary immersion system (TIS) for 12 weeks. Uniform young plants were selected and transferred to soilless mix in a growth chamber for ex vitro acclimatization during 35 days, followed by growing in a shaded greenhouse for 65 days. Comparison of in vitro leaf anatomy, ex vitro photosynthetic behaviors and growth was made between two cultural systems. Plants in TIS produced thicker leaf chlorenchyma and aquiferous parenchyma, lower stomatal frequency and more epicuticular wax than did those in semi-solid medium. Plants from semi-solid medium had consistently lower leaf Fv/Fm values than plants from TIS. Leaf Fv/Fm value in plants from TIS decreased to 0.65 at day 7 after transfer and increased soon up to 0.76 thereafter. In contrast, leaf Fv/Fm value in plants from semi-solid medium reduced to 0.27 at day 7 after transfer and increased slowly up to 0.68 at day 35. During ex vitro acclimatization, plants in TIS had substantial higher photosynthetic rates than plants in semi-solid medium. Plants from TIS had subsequent higher leaf area, fresh and dry weights than plants from semi-solid medium.

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Abbreviations

BAP:

6-Benzylaminopurine

MS:

Murashige and Skoog

TIS:

Temporary immersion system

References

  • Adams WW, Demmig-Adams B (2004) Chlorophyll fluorescence as a tool to monitor plant response to the environment. In: Papageorgiou GC, Govindjee (eds) Chlorophyll a fluorescence: a signature of photosynthesis. Springer, The Netherlands, pp 583–604

    Google Scholar 

  • Aragón C, Escalona M, Capote I, Pina D, Cejas I, Rodríguez R, Cañal MJ, Sandoval J, Roels S, Debergh P, González-Olmedo J (2005) Photosynthesis and carbon metabolism in plantain (Musa AAB) plantlets growing in temporary immersion bioreactors and during ex vitro acclimatization. In vitro Cell Dev Biol Plant 41:550–554

    Article  CAS  Google Scholar 

  • Bolhar-Nordenkampf HR, Long SP, Baker NR, Oquist G, Schreiber U, Lechner EG (1989) Chlorophyll fluorescence as a probe of the photosynthetic competence of leaves in the field: a review of current instrumentation. Funct Ecol 3:497–514

    Article  Google Scholar 

  • Cui YY, Hahn EJ, Kozai T, Paek KY (2000) Number of air exchanges, sucrose concentration, photosynthetic photon flux, and differences in photoperiod and dark period temperatures affect growth of Rehmannia glutinosa plantlets in vitro. Plant Cell Tissue Organ Cult 62:219–226

    Article  CAS  Google Scholar 

  • Dunston S, Sutter E (1984) In vitro propagation of prayer plants. HortScience 19:511–512

    CAS  Google Scholar 

  • Ebrahim MKH, Ibrahim IA (2000) Influence of medium solidification and pH value on in vitro propagation of Maranta leuconeura cv. Kerchoviana. Sci Hortic 86:211–221

    Article  CAS  Google Scholar 

  • Escalona M, Samson G, Borroto C, Desjardins Y (2003) Physiology of effects of temporary immersion bioreactors on micropropagated pineapple plantlets. In Vitro Cell Dev Biol Plant 39:651–656

    Article  CAS  Google Scholar 

  • Etienne H, Berthouly M (2002) Temporary immersion systems in plant micropropagaiton. Plant Cell Tissue Organ Cult 69:215–231

    Article  Google Scholar 

  • González-olmedo JL, Fundora Z, Molina LA, Abdulnour J, Desjardins Y, Escalona M (2005) New contributions to propagation of pineapple (Ananas comosus L. Merr.) in temporary immersion bioreactors. In vitro Cell Dev Biol Plant 41:87–90

    Article  Google Scholar 

  • Hazarika BN (2006) Morpho-physiological disorders in in vitro culture of plants. Sci Hortic 108:105–120

    Article  CAS  Google Scholar 

  • Krause G, Weis E (1991) Chlorophyll fluorescence and photosynthesis: the basics. Annu Rev Plant Physiol Plant Mol Biol 42:313–349

    Article  CAS  Google Scholar 

  • Lucchesini M, Monteforti G, Mensuali-Sodi A, Serra G (2006) Leaf ultrastructure, photosynthetic rate and growth of myrtle plantlets under different in vitro culture conditions. Biol Plant 50:161–168

    Article  Google Scholar 

  • Majada JP, Centeno ML, Feito I, Fernández B, Sánchez-Tamés R (1998) Stomatal and cuticular traits on carnation tissue culture under different ventilation conditions. Plant Growth Regul 25:113–121

    Article  CAS  Google Scholar 

  • Majada JP, Sierra MI, Sánchez-Tamés R (2001) Air exchange rate affects the in vitro developed leaf cuticle of carnation. Sci Hortic 87:121–130

    Article  Google Scholar 

  • Mohanty N, Vass I, Demeter S (1989) Copper toxicity affects photosystem II electron transport at the secondary quinone acceptor (QB). Plant Physiol 90:175–179

    Article  PubMed  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Paek KY, Chakrabarty D, Hahn EJ (2005) Application of bioreactor systems for large scale production of horticultural and medicinal plant. Plant Cell Tissue Organ Cult 81:287–300

    Article  Google Scholar 

  • Podwyszyńska M (1997) Micropropagation of Calathea ornata Koern. Biol Plant 39:179–186

    Article  Google Scholar 

  • Preece JE, Sutter EG (1991) Acclimatization in micropropagated plants to the greenhouse and field. In: Debergh PC, Zimmerman RH (eds) Micropropagation. Kluwer Academic Publishers, Dordrecht, pp 71–93

    Google Scholar 

  • Ritchie GA, Short KC, Davey MR (1991) In vitro acclimatization of chrysanthemum and sugar beet plantlets by treatment with paclobutrazol and exposure to reduced humidity. J Exp Bot 42:1557–1563

    Article  CAS  Google Scholar 

  • Rodríguze R, Cid M, Pina D, González-Olmedo JL, Desjardins Y (2003) Growth and photosynthetic activity during acclimatization of sugarcane plantlets cultivated in temporary immersion bioreactors. In vitro Cell Dev Biol Plant 39:657–662

    Article  Google Scholar 

  • Roels S, Noceda C, Escalona M, Sandoval J, Canal MJ, Rodriguez R, Debergh PC (2006) The effect of headspace renewal in a temporary immersion bioreactor on plantain (Musa AAB) shoot proliferation and quality. Plant Cell Tissue Organ Cult 84:155–163

    Article  Google Scholar 

  • Serret MD, Trillas MI (2000) Effects of light and sucrose levels on the anatomy, ultrastructure, and photosynthesis of Gardenia jasminoides Ellis leaflets cultured in vitro. Int J Plant Sci 161:281–289

    Article  PubMed  CAS  Google Scholar 

  • van Huylenbroeck JM, Piqueras A, Debergh PC (1998) Photosynthesis and carbon metabolism in leaves formed prior and during ex vitro acclimatization of micropropagated plants. Plant Sci 134:21–30

    Article  Google Scholar 

  • van Huylenbroeck JM, Piqueras A, Debergh PC (2000) The evolution of photosynthetic capacity and the antioxidant enzymatic system during acclimatization of micropropagated Calathea plants. Plant Sci 155:59–66

    Article  PubMed  Google Scholar 

  • Wetzstein HY, Sommer HE (1982) Leaf anatomy of tissue cultured Liquidambar styraciflua (Hamamelidaceae) during acclimatization. Am J Bot 69:1579–1586

    Article  Google Scholar 

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  1. Department of Horticulture, National Taiwan University, Taipei, Taiwan

    Shu-Han Yang & Der-Ming Yeh

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  1. Shu-Han Yang
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  2. Der-Ming Yeh
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Correspondence to Der-Ming Yeh.

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Yang, SH., Yeh, DM. In vitro leaf anatomy, ex vitro photosynthetic behaviors and growth of Calathea orbifolia (Linden) Kennedy plants obtained from semi-solid medium and temporary immersion systems. Plant Cell Tiss Organ Cult 93, 201–207 (2008). https://doi.org/10.1007/s11240-008-9363-3

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  • DOI: https://doi.org/10.1007/s11240-008-9363-3

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