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Use of liquid culture medium and ex vitro rooting for micropropagation of Acacia nilotica (L.) Del. ssp. cupressiformis

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Abstract

A micro-propagation method using liquid culture medium has been developed for Acacia nilotica ssp. cupressiformis. Nodal segments obtained from mature tree (15–20 years old) was used as explants for bud breaking and multiple shoot induction. MS liquid medium with 2.0 mg l−1 of 6-benzylaminopurine (BAP) was the best for shoot bud induction. Further shoots multiplication was achieved by subculturing of in vitro produced shoot clumps (2–3 shoots) in liquid medium supplemented with various concentrations of BAP. Maximum number of shoots (12.57 ± 0.37) and shoot length (10.11 ± 0.42) were recorded in MS liquid medium supplemented with 1.0 mg l−1 of BAP. About 90 % shoots rooted ex-vitro on soilrite within 3–4 weeks when base (3–5 mm) of shoots was treated with 300 mg l−1 of indole-3-butyric acid for 5 min. The plantlets were hardened successfully in the green house and transferred to the polybags. To the best of our knowledge, this is the first report of a micro-propagation protocol for A. nilotica ssp. cupressiformis.

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Acknowledgments

Authors (JSR, MP and MKR) wish to acknowledge the University Grants Commission (UGC) and Science and Engineering Research Board (SERB), New Delhi for the minor and major research projects and the award of Post Doctoral Fellowship and Young Scientists, respectively.

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Authors and Affiliations

  1. Department of Botany, Biotechnology Centre, Jai Narain Vyas University, Jodhpur, 342001, Rajasthan, India

    Jitendra Singh Rathore, Mahendra Phulwaria, Manoj K. Rai, Smita Shekhawat & N. S. Shekhawat

  2. Department of Botany and Biotechnology, L.M. College of Science and Technology (Autonomous), Jodhpur, 342001, Rajasthan, India

    Jitendra Singh Rathore & Mahendra Phulwaria

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  1. Jitendra Singh Rathore
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  2. Mahendra Phulwaria
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  3. Manoj K. Rai
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  4. Smita Shekhawat
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  5. N. S. Shekhawat
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Correspondence to Jitendra Singh Rathore.

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Rathore, J.S., Phulwaria, M., Rai, M.K. et al. Use of liquid culture medium and ex vitro rooting for micropropagation of Acacia nilotica (L.) Del. ssp. cupressiformis . Ind J Plant Physiol. 20, 172–176 (2015). https://doi.org/10.1007/s40502-015-0149-4

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