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Glass beads in liquid media: an alternative matrix for in vitro root induction of Cephaelis ipecacuanha A. Richard

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Abstract

Cephaelis ipecacuanha shoots were rooted in different concentrations of auxins on solid Murashige and Skoog (Physiol Plant 15:473–497, 1962) media and liquid MS media with glass beads. Glass beads were used as an alternative source of support matrix in liquid culture media to replace agar. The highest rooting response was observed on liquid MS medium with glass beads containing 2 mg l−1 α-naphthalene acetic acid within 15–20 days. Shoot elongation was also found to be better compared to solid MS medium. Rooted shoots were successfully hardened and established in the soil. Hence, an easy, reliable and reproducible protocol was developed for in vitro rooting of Ipecac on liquid MS medium with glass beads, which is biologically inert and reusable.

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Acknowledgments

We wish to express our thanks to Dr. George Patani of PSIR, Mumbai, for his guidance and encouragement during this research and Dr. Pramod Kumar Pandey of CIFE, Mumbai, for his help in the statistical analysis.

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Authors and Affiliations

  1. Dr. Patani Scientific and Industrial Research, PSIR Building, Inga Complex, Mahakali Road, Andheri (E), Mumbai, 400093, India

    Ved Prakash Pandey, Shanoli Ghosh, Elizabeth Cherian & Abraham Patani

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  1. Ved Prakash Pandey
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  2. Shanoli Ghosh
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  3. Elizabeth Cherian
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  4. Abraham Patani
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Correspondence to Ved Prakash Pandey.

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Pandey, V.P., Ghosh, S., Cherian, E. et al. Glass beads in liquid media: an alternative matrix for in vitro root induction of Cephaelis ipecacuanha A. Richard. Ind J Plant Physiol. 18, 388–391 (2013). https://doi.org/10.1007/s40502-014-0062-2

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  • DOI: https://doi.org/10.1007/s40502-014-0062-2

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