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A Liquid Culture System for Improved Micropropagation of Mature Acacia nilotica (L.) Del. ssp. indica and Ex Vitro Rooting

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

A micropropagation method using liquid culture medium has been developed for mature Acacia nilotica (L.) Del. ssp. indica. Nodal segments obtained from 15 to 20 years old mature trees were used as explants and cultured on 0.8 % agar-gelled Murashige and Skoog medium containing 6-benzylaminopurine for shoot bud induction. Once culture got established, explants were transferred to Murashige and Skoog liquid medium containing 6-benzylaminopurine or kinetin for shoot multiplication. Shoot multiplication was influenced by plant growth regulators, size of vessels, amount of medium in culture vessels and repeated transfer of mother explants. Murashige and Skoog liquid medium containing 4.4 μM 6-benzylaminopurine was found to be the best for shoot multiplication. The performance of liquid and agar-gelled medium for shoot multiplication was compared. About ten times increase in shoot number in liquid culture medium was achieved. Micropropagated shoots were rooted ex vitro. Shoots treated with 2.46 mM indole-3-butyric acid solution for 1 h followed by 1.41 mM chlorogenic acid for 5 min exhibited highest percent of rooting in the green house. This process of micropropagation of A. nilotica, can be utilized for plant production on a large-scale.

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Acknowledgments

The financial supports provided to the Department of Botany by University Grants Commissions (UGC), Department of Biotechnology (DBT) and the Department of Science and Technology (DST), Govt. of India are gratefully acknowledged. Authors (JSR, MKR and MP) wish to acknowledge the University Grants Commission (UGC), New Delhi for the minor research project and the award of DSKPDF and Post Doctoral Fellowship, respectively.

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Authors and Affiliations

  1. Department of Botany, Biotechnology Centre, Jai Narain Vyas University, Jodhpur, 342033, Rajasthan, India

    Jitendra Singh Rathore, Manoj K. Rai, Mahendra Phulwaria & N. S. Shekhawat

  2. Department of Botany and Biotechnology, L.M. College of Science and Technology (Autonomous), Jodhpur, 342001, Rajasthan, India

    Jitendra Singh Rathore

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  1. Jitendra Singh Rathore
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  2. Manoj K. Rai
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  3. Mahendra Phulwaria
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  4. N. S. Shekhawat
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Correspondence to Jitendra Singh Rathore.

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Rathore, J.S., Rai, M.K., Phulwaria, M. et al. A Liquid Culture System for Improved Micropropagation of Mature Acacia nilotica (L.) Del. ssp. indica and Ex Vitro Rooting. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 84, 193–200 (2014). https://doi.org/10.1007/s40011-013-0204-8

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  • DOI: https://doi.org/10.1007/s40011-013-0204-8

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