Abstract
Amylases are widely distributed and are one of the most studied enzymes. Such enzymes hydrolyze the starch molecules into polymers composed of glucose units. Amylases have potential application in a wide number of industrial processes such as food, fermentation and pharmaceutical industries. Amylases can be obtained from plants, animals and microorganisms. However, enzymes from fungal and bacterial sources have dominated applications in industrial sectors. The microbial source of amylase is preferred to other sources because of its plasticity and vast availability. The production of α-amylase is essential for conversion of starches into oligosaccharides. Starch is an important constituent of the human diet and is a major storage product of many economically important crops such as wheat, rice, maize, tapioca, and potato. The properties of each α-amylase such as thermostability, pH profile, pH stability, and Ca-independency are important in the development of fermentation process. This review focuses on the isolation, substrates of α-amylases, production of bacterial and fungal α-amylases, properties of α-amylases, and the use of these enzymes in industrial applications.
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References
Kathiresan K, Manivannana S (2006) α-Amylase production by Penicillium fellutanum isolated from mangrove rhizosphere soil. Afr J Biotechnol 5(10):829–832
Pandey A, Nigam P, Soccol CR, Soccol VT, Singh D, Mohan R (2000) Advances in microbial amylases. Biotechnol Appl Biochem 31:135–152
van der Marc Maarel, van der Veen Bart, Joost CM, Hans Uitdehaag, Leemhuis L, Dijkhuizen (2002) Properties and applications of starch converting enzymes of the α-amylase family. J Biotechnol 94:137–155
Chai YY, Rahman RN, Illias RM, Goh KM (2012) Cloning and characterization of two new thermostable and alkalitolerant α-amylases from the Anoxybacillus species that produce high levels of maltose. J Ind Microbiol Biotechnol 39:731–741
Anitha G, Muralikrighna G (2009) α-Amylase: structure and function relationship. Trends Carbohydr Res 1(4):1–11
Gupta R, Gigras P, Mohapatra H, Goswami VK, Chauhan B (2003) Microbial α-amylases: biotechnological perspective. Process Biochem 38:1599–1616
Sasi A, Baghyaraj R, Yogananth N, Chanthuru A, Ravikumar M (2008) Production of α-amylase in submerged fermentation by using Bacillus sp. Res J Biol Sci 1:50–57
Sivaramakrishnan S, Gangadharan V, Nampoothiri KM, Soccol CR, Pandey A (2006) α-Amylases from microbial sources—overview on recent developments. Food Technol Biotechnol 44(2):173–184
Lonsane BK, Ramesh MV (1990) Production of bacterial thermostable α-amylase by solid-state fermentation: potential tool for achieving economy in enzyme production and starch hydrolysis. Adv Appl Microbiol 35:1–56
Abu EA, Ado SA, James DB (2005) Raw starch degrading amylase production by mixed culture of Aspergillus niger and Saccharomyces cerevisiae grown on sorghum pomace. Afr J Biotechnol 4:785–790
Asgher M, Javaid M, Asad S, Rahman U, Legg RL (2007) Thermostable α-amylase from a moderately thermophilic Bacillus subtilis strain for starch processing. J Food Eng 79:950–955
Hema A, Ujjval T, Kamlesh P (2006) Alpha amylase production by Bacillus cereus MTCC 1305 using solid-state fermentation. Department of Biosciences, Patel University, Vidyanagar, pp 120–388
Joel EL, Bhimba BV (2012) Production of α-amylase by mangrove associated fungi Pestalotiopsis microspora strain VB5 and Aspergillus oryzae strain VBZ. Indian J Geo Marine Sci 41(3):279–283
Pandey S, Singh SP (2012) Organic solvent tolerance of α-amylase from haloalkaliphilic bacteria as a function of pH, temperature, and salt concentrations. Appl Biochem Biotechnol 166:1747–1757
Kadziola A, Sogaard M, Svensson B, Haser R (1998) Molecular structure of a barley alpha-amylase-inhibitor complex: implications for starch binding and catalysis. J Mol Biol 278:205–217
Machius M, Wiegand G, Huber R (1995) Crystal structure of calcium depleted Bacillus licheniformis α-amylase. J Mol Biol 246:545–559
Upadek H, Kottwitz B (1997) Surfactant science series: application of amylase in detergents. In: Ee JH, Misset O, Baas ET (eds) Enzymes in detergency. Marcel Dekker, New York, pp 203–212
Khan JA, Briscoe S (2011) Study on partial purification and characterization of extracellular alkaline amylase from Bacillus megaterium BY solid state fermentation. Int J Appl Biol Pharma Technol 2:3
Horikoshi K (1999) Alkaliphiles: some applications of their products for the Biotechnology. Microbiol Mol Biol Rev 63:735–750
Horikoshi K (1971) Production of alkaline enzyme by alkaliphilic microorganisms: alkaline amylase produced by Bacillus No. 221. Agric Biol Chem 35:1783–1791
Kaur P, Vyas A (2012) Characterization and optimal production of alkaline α-amylase from Bacillus sp. DLB 9. Afr J Microbiol Res 6(11):2674–2681
Yang H, Liu L, Li J, Du G, Chen J (2011) Heterologous expression, biochemical characterization and overproduction alkaline α-amylase from Bacillus alcalophilus in Bacillus subtilis. Microb Cell Fact 10:77
Walia A, Mehta P, Chauhan A, Shirkot CK (2012) Optimization of cellulase free xylanase production by alkalophilic Cellulosimicrobium cellulans strain CKMX1 in solid-state fermentation of apple pomace using central composite design and response surface methodology. Ann Microbiol. doi:10.1007/s13213-012-0460-5
Vasant KV (2010) Purification and properties of thermostable α-amylase from Acremonium sporosulcatum. Int J Biotecnol Biochem 6:25–34
Yaras A, Veyis S, Dursun O (2011) Production of α-amylase in semi solid substrate fermentation by Bacillus amyloliquefaciens. Curr Opin Biotechnol 10:1016
Haddaoui E, Chambert R, Petit-Glatron MF, Lindy O, Sarvas M (1999) Bacillus subtilis α-amylase: the rate limiting step of secretion is growth phase-independent. FEMS Microbiol Lett 173:127–131
Hamilton LM, Fogarty WM, Kelly CT (1999) Purification and properties of the raw starch degrading α-amylase of Bacillus sp. IMD 434. Biotechnol Lett 21:111–115
Pandey A, Soccol CR, Rodriguez-Leon JA, Nigam P (2001) Solid state fermentation in biotechnology: fundamentals and applications. Asia Tech Publishers Inc., New Delhi, pp 3–7
Kunamneni A, Permaul K, Singh S (2005) Amylase production in solid state fermentation by thermophilic fungus Thermomyces lanuginosus. J Biosci Bioeng 100(2):168–171
Bhattacharya S, Bhardwaj S, Das A, Anand S (2011) Utilization of sugarcane bagasse for solid-state fermentation and characterization of α-Amylase from Aspergillus flavus isolated from Muthupettai mangrove, Tamil Nadu, India. Aust J Basic Appl Sci 5(12):1012–1022
Suganthi R, Benazir JF, Santhi R, Ramesh Kumar V, Hari Anjana, Meenakshi Nitya, Nidhiya KA, Kavitha G, Lakshmi R (2011) Amylase production by Aspergillus Niger under solid state fermentation using agroindustrial wastes. Int J Eng Sci Technol 3(2):1756–1763
Dharania G, Kumaran NS (2012) Amylase Production from solid state fermentation and submerged liquid fermentation by Aspergillus niger. Bangladesh J Sci Ind Res 47(1):99–104
Baysal Z, Uyar F, Aytekin C (2003) Solid-state fermentation for production of α-amylase by a thermotolerant Bacillus subtilis from hot-spring water. Process Biochem 38:1665–1668
Ikram-ul-Haq, Ashraf H, Iqbal J, Qadeer MA (2003) Production of alpha amylase by Bacillus licheniformis using an economical medium. Bioresour Technol 38:8757–8761
Imran M, Asad MJ, Gulfraz M, Mehboob N, Jabeen N, Hadri SH, Irfan M, Anwar Z, Ahmed D (2011) Hyper production of glucoamylase by Aspergillus niger through chemical mutagenesis. Int J Phy Sci 6(26):6179–6190
Muhammad I, Anwar Z, Gulfraz M, Butt H, Ejaz A, Nawaj H (2012) Purification and characterization of α-amylase from Ganoderma tsugae growing in waste bread medium. Afr J Biotechnol 11(33):8288–8294
Ramesh MV, Lonsane BK (1991) Ability of a solid-state fermentation technique to significantly minimize catabolic repression of a-amylase production by Bacillus licheniformis M27. Appl Microbiol Biotechnol 35:591–593
Babu KR, Satyanarayana T (1995) α-Amylase production by thermophilic Bacillus coagulans in solid-state fermentation. Process Biochem 30:305–309
Carlsen M, Spohr AB, Nielsen J, Villadsen J (1996) Morphology and physiology of α-amylase producing strain of Aspergillus oryzae during batch cultivations. Biotechnol Bioeng 49:266–276
Spendler T, Jørgensen O (1997) Use of a branching enzyme in baking. Patent application WO97/41736
Bruinenberg PM, Hulst AC, Faber A, Voogd RH (1996) Process for surface sizing or coating of paper. European patent application EP 0 690 170 A1
Hendriksen HV, Pedersen S, Bisgard-Frantzen H (1999) Process for textile warp sizing using enzymatically modified starches. Patent application WO 99/35325
Vieille K, Zeikus GJ (2001) Hyperthermophilic enzymes: sources, uses, and molecular mechanisms of thermostability. Microbiol Mol Biol Rev 65:1–43
Vihinen M, Mantsala P (1989) Microbial amylolytic enzymes. Crit Rev Biochem Mol Biol 24:329–418
Schwermann B, Pfau K, Liliensiek B, Schleyer M, Fischer T, Bakker EP (1994) Purification, properties and structural aspects of a thermoacidophilic α-amylase from Alicyclobacillus acidocaldarius ATCC 27009 insight into acidostability of proteins. Eur J Biochem 226:981–991
Hassan S, Naderi-Manesh H, Khajeh K, Ahmadvand R, Ranjbar B, Asoodeh A, Moradian F (2005) Ca-independent α-amylase that is active and stable at low pH from the Bacillus sp. KR-8104. Enzyme Microb Technol 36:666–671
Kim TU, Gu BG, Jeong JY, Byun SM, Shin YC (1995) Purification and characterization of a maltotetraose forming alkaline amylase from an alkalophilic Bacillus sp. GM8901. Appl Environ Microbiol 61:3105–3112
Khajeh K, Shokri MM, Asghari SM, Moradian F, Ghasemi A, Sadeghi M, Ranjbar B, Hosseinkhani S, Gharavi S, Naderi-Manesh H (2006) Acidic and proteolytic digestion of α-amylases from Bacillus licheniformis and Bacillus amyloliquefaciens: stability and flexibility analysis. Enzyme Microb Technol 38:422–428
Burhan A, Nisa U, Gokhan C, Omer C, Ashabil A, Osman G (2003) Enzymatic properties of a novel thermostable, thermophilic, alkaline and chelator resistant amylase from an alkaliphilic Bacillus sp. isolate ANT-6. Process Biochem 38:1397–1403
Mohamed Saleh A, Azhar Esam I, Ba-Akdah Morooj M, Tashkandy Nisreen R, Kumosanil Taha A (2011) Production, purification and characterization of α-amylase from Trichoderma harzianum grown on mandarin peel. Afr J Microbiol Res 5(9):1018–1028
Emmanuel L, Janecek S, Haye B, Belarbi A (2000) Thermophilic archaeal amylolytic enzymes. Enzyme Microb Technol 26:3–14
Elayaraja ST, Velvizhi V, Maharani P, Mayavu SV, Balasubramanian T (2011) Thermostable α-amylase production by Bacillus firmus CAS 7 using potato peel as a substrate. Afr J Biotechnol 10(54):11235–11238
Khwaja S, Prasad R, Kumar S, Visavadia MD (2011) Isolation of soil thermophilic strains of actinomycetes for the production of α-amylase. Afr J Biotechnol 10(77):17831–17836
Kobayashi T, Kamekura M, Kanlayakrit W, Ohnishi H (1986) Production, purification and characterization of an amylase from the moderate halophile Micrococcus varians subspecies halophilus. Microbios 46:165
Coronado MJ, Vargas C, Hofemeister J, Ventosa A, Nieto JJ (2000) Production and biochemical characterization of an α-amylase from the moderate halophile Halomonas meridiana. FEMS Microbiol Lett 183:67–71
Bush DS, Sticher L, Huystee RV, Wagner D, Jones RL (1989) Calcium requirement for stability and enzymatic activity of two isoforms of barley aleuron alpha amylase. J Biol Chem 264:19392–19398
Nielsen Anders D, Fuglsang Claus C, Westh Peter (2003) Effect of calcium ions on the irreversible denaturation of a recombinant Bacillus halmapalus α-amylase: calorimetric investigation. Biochem J 373:337–343
Atsushi T, Eiichi H (2002) Calcium-binding parameter of Bacillus amyloliquefaciens α-amylase determined by inactivation kinetics. Biochem J 364:635–639
Goyal N, Gupta JK, Soni SK (2005) Novel raw starch digesting thermostable α-amylase from Bacillus sp. I-3 and its use in the direct hydrolysis of raw potato starch. Enzyme Microb Technol 37:723–734
Heinen W, Lauwers AM (1975) Amylase activity and stability at high and low temperature depending on calcium and other divalent cations. Experientia 26:77
Robyt J, French D (1963) Action pattern and specificity of an amylase from Bacillus subtilis. Arch Biochem Biophys 100:451–467
Laderman KA, Davis BR, Krutzsch HC, Lewis MS, Griko YV, Privalov PL, Anfinsen CB (1993) Purification and characterization of an extremely thermostable α-amylase from hypothermophilic archaebacterium Pyrococcus furiosus. J Biol Chem 993(268):24394–24401
Malhotra R, Noorvez SM, Satyanarayana T (2000) Production and partial characterization of thermostable and calcium independent alpha amylase of an extreme thermophile Bacillus thermooleovorans NP54. Lett Appl Microbiol 31:378–384
Yang CH, Liu WH (2004) Purification and properties of a maltotriose producing α-amylase from Thermobifida fusca. Enzyme Microb Technol 35:254–260
Najafi MF, Kembhavi A (2005) One-step purification and characterization of an extracellular α-amylase from marine Vibrio sp. Enzyme Microb Technol 36:535–539
Shigechi H, Fujita Y, Koh J, Ueda M, Fukuda H, Kondo A (2004) Energy saving direct ethanol production from low temperature cooked corn starch using a cell-surface engineered yeast strain co-displaying glucoamylase and α-amylase. Biochem Eng J 18:149–153
Kumar DJ, Jayanthisiddhuraj M, Monica DD, Naganarayani K, Immaculate A, Rebecca N, Kalaichelvan PT (2012) Concomitant production of α-amylase and β-galactosidase by native Bacillus sp. MNJ23 isolated from dairy effluent. Am Eurasian J Agric Environ Sci 12(5):579–587
Thippeswamy S, Girigowda K, Mulimani VH (2006) Isolation and identification of α-amylase producing Bacillus sp. from dhal industry waste. Indian J Biochem Biophys 43:295–298
Acourene S, Ammouche A (2012) Optimization of ethanol, citric acid, and α-amylase production from date wastes by strains of Saccharomyces cerevisiae, Aspergillus niger, and Candida guilliermondii. J India Microbiol Biotechnol 39:759–766
Ganiyu O (2005) Isolation and characterization of amylase from fermented cassava (Manihot esculenta Crantz) wastewater. Afr J Biotechnol 4(10):1117–1123
Domingues CM, Peralta RM (1993) Production of amylase by soil fungi and partial biochemical characterization of amylase of a selected strain Aspergillus fumigatus Fresenius. Can J Microbiol 39:681–685
Arnesen S, Eriksen SH, Olsen J, Jensen B (1998) Increased production of alpha amylase from Thermomyces lanuginosus by the addition of Tween-80. Enzyme Microb Technol 23:249–252
Textor SD, Hill GA, Macdonald DG, Denis ESt (1998) Cold enzyme hydrolysis of wheat starch granules. Can J Chem Eng 76:87–93
Robertson GH, Wong DWS, Lee CC, Wagschal K, Smith MR, Orts WJ (2006) Native or raw starch digestion: key step in energy efficient biorefining of grain. J Agric Food Chem 54:353–365
Liao B, Hill GA, Roesler WJ (2012) Stable expression of barley α-amylase in S. cerevisiae for conversion of starch into bioethanol. Biochem Eng J 64:8–16
Nielsen JE, Borchert TV (2000) Protein engineering of bacterial alpha-amylases. Biochem Biophys Acta 1543:253–274
Prakash O, Jaiswal N (2010) Alpha-amylase: ideal representative of thermostable enzymes. Appl Biochem Biotechnol 160(8):2401–2414
Mukherjee AK, Borah M, Rai SK (2009) To study the influence of different components of fermentable substrates on induction of extracellular α-amylase synthesis by Bacillus subtilis DM-03 in solid state fermentation and exploration of feasibility for inclusion of α-amylase in laundry detergent formulations. Biochem Eng J 43:149–156
Olsen HSO, Falholt P (1998) Role of Enzymes in Modern Detergency. J Surfactants Deterg 1:555–567
Hmidet N, El-Hadj Ali N, Haddar A, Kanoun S, Alya S, Nasri M (2009) Alkaline proteases and thermostable α-amylase co-produced by Bacillus licheniformis NH1: characterization and potential application as detergent additive. Biochem Eng J 47:71–79
Mitidieri S, Souza Martinelli AH, Schrank A, Vainstein MH (2006) Enzymatic detergent formulation containing amylase from Aspergillus niger: a comparative study with commercial detergent formulations. Bioresour Technol 97:1217–1224
Kirk O, Borchert TV, Fuglsang CC (2002) Industrial enzyme applications. Curr Opin Biotechnol 13:345–351
Ahlawat S, Dhiman SS, Battan B, Mandhan RP, Sharma J (2009) Pectinase production by Bacillus subtilis and its potential application in biopreparation of cotton and micropoly fabric. Process Biochem 44:521–526
Feitkenhauer H (2003) Anaerobic digestion of desizing wastewater: influence of pretreatment and anionic surfactant on degradation and intermediate accumulation. Enzyme Microb Technol 33:250–258
Saxena RK, Malhotra B, Batra A (2004) Commercial importance of some fungal enzymes. In: Arora DK (ed) Handbook of fungal biotechnology. Marcel Dekker, New York, pp 287–298
Couto SR, Sanromán MA (2006) Application of solid-state fermentation to food industry—review. J Food Eng 76:291–302
Ghorai S, Banik SP, Verma D, Chowdhury S, Mukherjee S, Khowala S (2009) Fungal biotechnology in food and feed processing. Food Res Int 42:577–587
Gavrilescu M, Chisti Y (2005) Biotechnology-sustainable alternative for chemical industry. Biotechnol Adv 23:471–499
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Rana, N., Walia, A. & Gaur, A. α-Amylases from Microbial Sources and Its Potential Applications in Various Industries. Natl. Acad. Sci. Lett. 36, 9–17 (2013). https://doi.org/10.1007/s40009-012-0104-0
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DOI: https://doi.org/10.1007/s40009-012-0104-0