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Preparation of Drug Eluting Natural Composite Scaffold Using Response Surface Methodology and Artificial Neural Network Approach

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Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

Silk fibroin/xanthan composite was investigated as a suitable biomedical material for controlled drug delivery, and blending ratios of silk fibroin and xanthan were optimized by response surface methodology (RSM) and artificial neural network (ANN) approach. A non-linear ANN model was developed to predict the effect of blending ratios, percentage swelling and porosity of composite material on cumulative percentage release. The efficiency of RSM was assessed against ANN and it was found that ANN is better in optimizing and modeling studies for the fabrication of the composite material. In-vitro release studies of the loaded drug chloramphenicol showed that the optimum composite scaffold was able to minimize burst release of drug and was followed by controlled release for 5 days. Mechanistic study of release revealed that the drug release process is diffusion controlled. Moreover, during tissue engineering application, investigation of release pattern of incorporated bioactive agent is beneficial to predict, control and monitor cellular response of growing tissues. This work also presented a novel insight into usage of various drug release model to predict material properties. Based on the goodness of fit of the model, Korsmeyer–Peppas was found to agree well with experimental drug release profile, which indicated that the fabricated material has swellable nature. The chloramphenicol (CHL) loaded scaffold showed better efficacy against gram positive and gram negative bacteria. CHL loaded SFX55 (50:50) scaffold shows promising biocomposite for drug delivery and tissue engineering applications.

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Acknowledgements

The authors are grateful to School of Biochemical Engineering, Indian Institute of Technology (Banaras Hindu University) and Ministry of Human Resource and Development, Government of India, for providing financial support in terms of fellowship, research facilities, and infrastructure for carrying out the present research work.

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Authors and Affiliations

  1. Bioprocess Technology Laboratory, School of Biochemical Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi, Lanka, Varanasi, Uttar Pradesh, 221005, India

    Shailendra Singh Shera, Shraddha Sahu & Rathindra Mohan Banik

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  1. Shailendra Singh Shera
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  2. Shraddha Sahu
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  3. Rathindra Mohan Banik
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Correspondence to Rathindra Mohan Banik.

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Shera, S.S., Sahu, S. & Banik, R.M. Preparation of Drug Eluting Natural Composite Scaffold Using Response Surface Methodology and Artificial Neural Network Approach. Tissue Eng Regen Med 15, 131–143 (2018). https://doi.org/10.1007/s13770-017-0100-z

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  • DOI: https://doi.org/10.1007/s13770-017-0100-z

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