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Expression analysis of an APETALA1/FRUITFULL-like gene in Phalaenopsis sp. ‘Hatsuyuki’ (Orchidaceae)

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Abstract

Members of the APETALA1 (AP1)/FRUITFULL (FUL)-like gene family of MADS-box genes play important roles in controlling the development of floral organs. To understand the molecular mechanisms of floral development in orchid, we isolated and characterized a Phalaenopsis AP1/FUL-like gene, PhalFUL. The results of phylogenetic analysis indicated that PhalFUL is in the monocots group of AP1/FUL-like gene. PhalFUL transcripts were detected in the flower buds, but not in vegetative organs. Moreover, in situ hybridization experiments revealed PhalFUL hybridization signals in all floral organ primordia at a very early stage of floral development, and continued expression in the column of whorls 3 and 4 until late developmental stages. These expression patterns were similar to those of the FUL-like genes in Arabidopsis (FUL) and Antirrhinum (DEFH28), suggesting that the PhalFUL is similar in function to FUL and DEFH28.

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Authors and Affiliations

  1. Subtropical Horticulture Research Institute, Jeju National University, Jeju, 690-756, Korea

    In-Ja Song, Suk-Min Ko & Hyo-Yeon Lee

  2. Faculty of Biotechnology, Jeju National University, Jeju, 690-756, Korea

    In-Ja Song & Hyo-Yeon Lee

  3. Faculty of Agriculture, Kochi University, Kochi, 783-8502, Japan

    Tatsuya Fukuda

  4. Institute for Advanced Biosciences, Keio University, Yamagata, 997-0052, Japan

    Takuro Ito

  5. Faculty of Science, Yamagata University, Yamagata, 990-8560, Japan

    Jun Yokoyama

  6. Akabira Orchid Corp., Akabira, 079-1273, Japan

    Hiroyuki Ichikawa

  7. Obihiro University of Agriculture and Veterinary Medicine, Obihiro, 080-8555, Japan

    Yoh Horikawa

  8. Graduate School of Life Sciences, Tohoku University, Sendai, 980-8577, Japan

    Toshiaki Kameya & Akira Kanno

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  1. In-Ja Song
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Song, IJ., Fukuda, T., Ko, SM. et al. Expression analysis of an APETALA1/FRUITFULL-like gene in Phalaenopsis sp. ‘Hatsuyuki’ (Orchidaceae). Hortic. Environ. Biotechnol. 52, 183–195 (2011). https://doi.org/10.1007/s13580-011-0199-0

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