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Molecular cloning and spatiotemporal expression of APETALA1-like gene in Lonicera macranthoides

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Abstract

APETALA1 (AP1), a floral meristem identity gene controls the flowering time and floral transition, and plays an important role in inflorescence and floral organ development. The full-length cDNA for AP1 was obtained by rapid amplification of the cDNA ends (RACE) so that the roles of AP1 in Lonicera macranthoides (Lm-AP1) could be better understood. AP1 (accession number in GenBank: MF418642) consisted of a 729-bp open reading frame encoding a protein that contained 242 amino acids, had a deduced molecular mass of 27.9919 kDa and a theoretical isoelectric point of 8.75. No signal peptide or transmembrane domains were detected in the sequences located in the nucleus, but it contained conserved sequences for MADS and the K-box. In the secondary structure, the \(\alpha \)-helix accounts for 60.74%, the \(\beta \)-turn 3.72%. The real-time polymerase chain reaction revealed that AP1 was more highly expressed in flowers, especially at the fourth flowering stage, which implied that it may play a role in flower development. Other L. macranthoides organs, such as stems and leaves, also expressed AP1. This research provided the basis for further analysis of the AP1 functional mechanism during L. macranthoides development.

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References

  • Arnold K., Bordoli L., Kopp J. and Schwede T. 2006 The SWISS-MODEL workspace: a web-based environment for protein structure homology modelling. Bioinformatics 22, 195–201.

    Article  CAS  Google Scholar 

  • Cai J. L. 2016 Cloning and expression of AGL15 gene related to prolong the buds from Lonicera macranthoides. Changsha: Hunan Univ. Chin. Med.

    Google Scholar 

  • Chen M. K., Lin I. C. and Yang C. H. 2008 Functional analysis of three lily (Lilium longiflorum) APETALA1-like MADS box genes in regulating floral transition and formation. Plant Cell Physiol. 49, 704–717.

    Article  CAS  Google Scholar 

  • Gasteiger E., Hoogland C., Gattiker A., Duvaud S., Wilkins M. R., Appel R. D. et al. 2005 Protein identification and analysis tools on the ExPASy Server, pp. 571–607. The Proteomics Protocols Handbook, Humana Press.

  • Guan F. Q., Liu M., Shan Y., Chen Y., Zhao Y. Y., Wang M. et al. 2013 The antioxidant activity evaluations of the triterpene saponins from Lonicera macranthoides in vitro, Lishizhen. Med. Mater. Med. Res. 24, 1315–1317.

    CAS  Google Scholar 

  • Guo W. Y., Cui Y. M., Yu D. Y. and Huang F. 2016 Cloning and functional analysis of GsAP1 in wild soybean. Soybean Sci. 35, 919–927.

    Google Scholar 

  • Han Y., Zhang C., Yang H. and Jiao Y. Cytokinin pathway mediates APETALA1 function in the establishment of determinate floral meristems in Arabidopsis. Proc. Natl. Acad. Sci. USA 111, 6840–6845.

  • He S. L., Meng J., Hong M. W., Li Y., Zhang X. and Yang Y. 2015 Max Ent-based prediction distribution areas of Lonicera japonica and Lonicera macranthoides. Yunan J. Agric. Univ. 30, 777–783.

    Google Scholar 

  • He C. P., He T., Zhang L., Tian T. X. and She C. W. 2016 Effect of different processing methods and harvest time on the quality of Lonicera macranthoides. in Hunan. Chin. J. Pharm. 39, 1817–1819.

  • Hou Q., Tang Q., Zhang X. N., Li Y. and Xu X. Y. 2013 Comparative studies on pharmacodynamics of Lonicera macranthoides and Lonicera japonica, Chin. Tradit. Herb. Drugs 44, 309–314.

    CAS  Google Scholar 

  • Koornneef M., Dellaert L. W. and van der Veen J. H. 1982 EMS and radiation-induced mutation frequencies at individual loci in Arabidopsis thaliana (L.) Heynh. Mutat. Res. 93, 109–123.

    Article  CAS  Google Scholar 

  • Lam H. M., Xu X., Liu X., Chen W., Yang G., Wong F. L. et al. 2010 Resequencing of 31 wild and cultivated soybean genomes identifies patterns of genetic diversity and selection. Nat. Genet. 42, 1053–1059.

    Article  CAS  Google Scholar 

  • Liu J. 2016 Studies on the difference of antiviral effect and spectrum-effect relationships of efficacy components between Lonicerae Japonicae Flos. and Lonicerae Flos. Beijing: Beijing Univ. of Chin. Med.

  • Liu M. and Lai Z. X. 2012 Cloning and bioinformatics analysis of AP1 gene from the leaves of in vitro plantlets in Tagetes patula L.. Chin. J. Trop. Crop. 33, 1778–1783.

    Google Scholar 

  • Liu C., Zhou J., Bracha-Drori K., Yalovsky S., Ito T. and Yu H. 2007 Specification of Arabidopsis floral meristem identity by repression of flowering time genes. Development 134, 1901–1910.

    Article  CAS  Google Scholar 

  • Liu D., Sun X., Mu Q., Wu W. M., Zhang Z. and Fang J. G. 2015 Analysis of expression levels of floral genes in the buds on different branch nodes of Grapevine. Sci. Agric. Sin. 48, 2007–2016.

    CAS  Google Scholar 

  • Mandel M., Gustafson-Brown C., Savidge B. and Yanofsky M. F. 1992 Molecular characterization of the Arabidopsis floral homeotic gene APETALA. Nature 360, 273–277.

    Article  CAS  Google Scholar 

  • Münster T., Pahnke J., Di Rosa A., Kim J. T., Martin W., Saedler H. et al. 1997 Floral homeotic genes were recruited from homologous MADS-box genes preexisting in the common ancestor of ferns and seed plants. Proc. Natl. Acad. Sci. USA 94, 2415–2420.

  • Petersen T. N., Brunak S., von Heijne G. and Nielsen H. 2011 SignalP 4.0: discriminating signal peptides from transmembrane regions. Nat. Methods 8, 785–786.

    Article  CAS  Google Scholar 

  • Sawettalake N., Bunnag S., Wang Y., Shen L. and Yu H. DOAP1 promotes flowering in the orchid Dendrobium Chao Praya Smile. Front Plant Sci. 8, 400.

  • Sun M. S., Zhou R. B., Peng X. D., Wang S., Liu X. D. and Wang Z. H. 2013 Dynamic change of endogenous ethylene in wild and cultivar Xianglei of Lonicera macranthoides in flowering periods. Chin. Tradit. Pat. Med. 35, 1969–1972.

    CAS  Google Scholar 

  • Tan C. 2013 The functional analysis of AP1 homologous gene in Mango flower organs development. Nanning: Guangxi Univ.

    Google Scholar 

  • Wang X. Y. 2015 Cloning and expression analysis of ZFP-l gene associated with the flower tube cracking from Lonicera macranthoides. Changsha: Central South Univ. Forestry Technol.

    Google Scholar 

  • Wu Y. Q., Ge J. T. and Tao J. 2015 cDNA cloning, sequence analysis and tissue expression detection of APETALA1 gene (AP1) in Paeonia lactiflora Pall. Petals of different development stages. J. Agric. Biotechnol. 23, 1559–1567.

    CAS  Google Scholar 

  • Xu J., Zhang Y., Cui G. L., She Y. H. and Li L. Y. 2016 Study on influence factors of seed germination and seeding growth of Lonicera macranthoides. China J. Chin. Mater. Med. 41, 51–55.

    Google Scholar 

  • Xu Y. Q., Wang S., Liu X. D., Tong Q. Z., Zhou R. B. and Sun M. S. 2015 Study on methods for extraction of total RNA from flower bud of Lonicera macranthoides.. Guid. J. Tradit. Chin. Med. Pharm. 21, 31–34.

    Google Scholar 

  • Yi G. Q., Cai J. L., Zhu Y. L., Chen L., Fan Y., Liu F. et al. 2016 Cloning of MADS-box gene AGL15 from L. macranthoides and analysis on its bioinformatics and expression. Chin. Tradit. Herb. Drugs 47, 640–647.

    CAS  Google Scholar 

  • Zhang X. N. 2014 Comparative studies on the chemical components and pharmacological effects of L. macranthoides Hand.-Mazz. and Lonicera japonica Thunb. Chongqing: Southwest Univ.

  • Zhang X. N., Tang Q., Huo M., Li Y. and Xu X. Y. 2012 Comparison of the pharmacological effects between L. macranthoides Hand.-Mazz. and Lonicera japonica Thunb. Chin. Pharmacol. Bull. 28, 1582–1585.

    Google Scholar 

  • Zhang L., Li Y. E., Guan S. M., Liu C. Y., Gai S. P. and Zhang Y. X. 2015 Expression analysis and functional identification of PsAP1 in tree peony. Acta Agric. Boreali-Sin. 30, 84–89.

    Google Scholar 

  • Zou D. M., Liu Y. X., Zhang Z. H., Li H., Ma Y. and Dai H. Y. 2012 Cloning, expression and promoter analysis of AP1 homologous gene from strawberry (Fragariaxananassa). Sci. Agric. Sin. 45, 1972–1981.

    CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by ‘the Chinese National Natural Science Foundation’ (grant numbers: 81203007 and 81673546), ‘The Natural Science Foundation of China Hunan Province’ (grant number: 2017JJ3237), ‘The Youth Foundation of China Hunan Provincial Education Department’ (grant number: 16B193), ‘The Key Disciplines Foundation Program of Chinese Medicine in China Hunan Province’ (grant number: zy201505), ‘Hunan Province Universities 2011 Collaborative Innovation Center of Protection and Utilization of Hu-xiang Chinese Medicine Resources’ (Xiang teach [2015] no. 351). We thank International Science Editing for editing this manuscript.

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Authors and Affiliations

  1. College of Pharmacy, Hunan University of Chinese Medicine, Changsha, People’s Republic of China

    Shan Wang, Mei Chen Peng, Xun Chen, Chang Yu Liu, Ya Chen, Xiang Dan Liu & Ri Bao Zhou

  2. Pharmacy, The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, People’s Republic of China

    Shan Wang

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  1. Shan Wang
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  2. Mei Chen Peng
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  3. Xun Chen
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  4. Chang Yu Liu
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  5. Ya Chen
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  6. Xiang Dan Liu
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  7. Ri Bao Zhou
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Correspondence to Xiang Dan Liu or Ri Bao Zhou.

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Corresponding editor: Qingpo Liu

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Wang, S., Peng, M.C., Chen, X. et al. Molecular cloning and spatiotemporal expression of APETALA1-like gene in Lonicera macranthoides. J Genet 97, 1281–1288 (2018). https://doi.org/10.1007/s12041-018-1025-6

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  • DOI: https://doi.org/10.1007/s12041-018-1025-6

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