Skip to main content
SpringerLink
Log in

Production of polyhydroxybutyrate (PHB) by a novel Klebsiella pneumoniae strain using low-cost media from fruit peel residues

  • Original Article
  • Published:
Biomass Conversion and Biorefinery Aims and scope Submit manuscript

Abstract

Plastics are widely used for various applications. Once discarded, it is commonly known that they represent a high environmental threat due to their slow degradation; for this reason, there is an imminent need to replace these products with eco-friendlier ones. In the present work, four bacterial polyhydroxybutyrate (PHB) producers, two consortia, and two isolated strains were successfully recovered from the facilities of a paper-manufacturing industry. Spectroscopic studies of the biopolymers obtained from these bacteria corroborated their PHB production capabilities, ranging from 4.04 ± 0.16 to 23.82 ± 3.39 g/L. The characterization of the isolate that presented the highest production yield initially coded as E22 led to the identification of a Klebsiella pneumoniae strain, which, compared with other PHA bacterial producers reported to date, could be considered with high production potential. The strain E22 was grown in 5 different media prepared from fruit peel residues of banana, orange, papaya, watermelon, and melon, to determine its growth and PHA production capabilities in these low-cost media. The results obtained show different bacterial growth yields among the media tested, although PHB production yields and productivities were similar in all these low-cost media. Cellular accumulation of the biopolymer was higher in watermelon peel medium (8.4 × 10−10 g/CFU). These results reveal the potential of K. pneumoniae E22 for PHB production applications and establish encouraging alternatives to be broader explored regarding low-cost media that could enhance the scale-up of bacterial PHA production processes.

Graphical abstract

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Al-Salem SM, Lettieri P, Baeyens J (2010) The valorization of plastic solid waste (psw) by primary to quaternary routes: from re-use to energy and chemicals. Prog Energ Combust 36:103–129. https://doi.org/10.1016/j.pecs.2009.09.001.2010

  2. Andrady AL, Neal MA (2009) Applications and societal benefits of plastics. Philos Trans R Soc Lond Ser B Biol Sci 364:1977–1984. https://doi.org/10.1098/rstb.2008.0304

    Article  Google Scholar 

  3. Devasahayam S, Raman RKS, Chennakesavulu K, Bhattacharya S (2019) Plastics-villain or hero? Polymers and recycled polymers in mineral and metallurgical processing-a review. Materials 12:1–36. https://doi.org/10.3390/ma12040655

    Article  Google Scholar 

  4. Sukan A, Roy I, Keshavarz T (2015) Dual production of biopolymers from bacteria. Carbohydr Polym 1:47–51. https://doi.org/10.1016/j.carbpol.2015.03.001

    Article  Google Scholar 

  5. Šprajcar M, Horvat P, Krzan A (2012) Biopolymers and bioplastics–plastics aligned with nature. European Regional Development Fund. https://www.umsicht.fraunhofer.de/content/dam/umsicht/de/ dokumente/ueber-uns/nationale-infostelle-nachhaltige-kunststoffe/biopolymers-bioplastics-brochure-for-teachers.pdf. Accessed 14 February 2020

  6. Serwanska-Leja K, Lewandowicz G (2010) Polymer biodegradation and biodegradable polymers-a review. Pol J Environ Stud 19:255–266. https://pdfs.semanticscholar.org/72a6/fb58bf5809b42af5861af6dd2541941e7858.pdf?_ga=2.69194769.1238613553.1588290677-1430679523.1583797552. Accessed 14 February 2020

  7. Krueger CL, Radetski CM, Bendia AG, Oliveira IM, Castro-Silva MA, Rambo CR, Antonio RV, Lima AOS (2012) Bioconversion of cassava starch by-product into Bacillus and related bacteria polyhydroxyalkanoates. Electron J Biotechnol 15:1–13. https://doi.org/10.2225/vol15-issue3-fulltext-6

    Article  Google Scholar 

  8. Sinha K, Rathore P (2015) Study of polyhydroxybutyrate producing Bacillus sp. isolated from soil. Res J Recent Sci 4:61–69. http://www.isca.in/rjrs/archive/v4/iIYSC-2015/12.ISCA-IYSC-2015-3BS-09.pdf. Accessed 18 February 2020

  9. Mohapatra S, Maity S, Dash HR, Das S, Pattnaik S, Rath CC, Samantaray D (2017) Bacillus and biopolymer: prospects and challenges. Biochem Biophys Rep 12:206–213. https://doi.org/10.1016/j.bbrep.2017.10.001

    Article  Google Scholar 

  10. Shah KR (2014) Optimization and production of polyhydroxybutarate (PHB) by Bacillus subtilis G1S1 from soil. Int J Curr Microbiol App Sci 3:377–387. https://www.ijcmas.com/vol-3-5/K.R.Shah.pdf. Accessed 9 March 2020

  11. Apparao U, Krishnaswamy VG (2015) Production of polyhydroxyalkanoate (PHA) by a moderately halotolerant bacterium Klebsiella pneumoniae U1 isolated from rubber plantation area. Int J Environ Biorem Biodegrad 3:54–61. https://doi.org/10.12691/ijebb-3-2-3

    Article  Google Scholar 

  12. Chen BY, Shiau TJ, Wei YH, Chen WM (2012) Feasibility study on polyhydroxybutyrate production of dye-decolorizing bacteria using dye and amine-bearing cultures. J Taiwan Inst Chem Eng 43:241–245. https://doi.org/10.1016/j.jtice.2011.09.001

    Article  Google Scholar 

  13. Eiamsa-ard P (2017) Isolation and identification of polyhydroxyalkanoate (PHA) producing bacteria from food industrial wastewater by using fluorometric screening. Journal of KMUTNB 27:771–781. https://doi.org/10.14416/j.kmutnb.2017.11.004

    Article  Google Scholar 

  14. Menaga M, Felix S, Gopalakannan A (2019) Screening of a biodegradable polymer, polyhydroxy butyrate (PHB) accumulating bacteria from the biofloc based gift tilapia ponds. Aquaculture 2019 - meeting abstract; World Aquaculture Society. https://www.was.org/meetings/ShowAbstract.aspx?Id= 136449. Accessed 9 March 2020

  15. Narayankumar S, Krishnaswamy VG (2017) Characterization and optimization studies on poly[(R)-3-hydroxybutyrate] producing moderately halotolerant bacterial strains isolated from saline environment. J Biodivers Manage Forestry 6:3. https://doi.org/10.4172/2327-4417.1000181

    Article  Google Scholar 

  16. Peña C, Castillo T, García A, Millán M, Segura D (2014) Biotechnological strategies to improve production of microbial poly-(3-hydroxybutyrate): a review of recent research work. Microb Biotechnol 7:278–293. https://doi.org/10.1111/1751-7915.12129.4

    Article  Google Scholar 

  17. Pillai AB, Kumar AJ, Kumarapillai H (2018) Enhanced production of poly (3-hydroxybutyrate) in recombinant Escherichia coli and EDTA-microwave-assisted cell lysis for polymer recovery. AMB Express 8:1–15. https://doi.org/10.1186/s13568-018-0672-6

    Article  Google Scholar 

  18. Sadaf N, Kamthane DC (2019) PHB production from bacteria isolated from oil contaminated soil. Bioscience Discovery 10:103-107. http://biosciencediscovery.com/Vol%2010%20No%202/Nausheen103-107.pdf. Accessed 9 March 2020

  19. Tufail S, Munir S, Jamil N (2017) Variation analysis of bacterial polyhudroxyalkanoates production using saturated and unsaturated hydrocarbons. Braz J Microbiol 48:629–636. https://doi.org/10.1016/j.bjm.2017.02.008

    Article  Google Scholar 

  20. Zhang H, Obias V, Gonyer K, Dennis D (1994) Production of polyhydroxyalkanoates in sucrose-utilizing recombinant Escherichia coli and Klebsiella strains. Appl Environ Microbiol 60:1198–1205. https://aem.asm.org/content/aem/60/4/1198.full.pdf. Accessed 19 February 2020

  21. Kourmentza C, Plácido J, Venetsaneas N, Burniol-Figols A, Varrone C, Gavala HN, Reis MAM (2017) Recent advances and challenges towards sustainable polyhydroxyalkanoate (PHA) production. Bioengineering (Basel) 4:1–43. https://doi.org/10.3390/bioengineering4020055

    Article  Google Scholar 

  22. Guerra-Blanco P, Cortes O, Poznyak T, Chairez I, García-Peña EI (2018) Polyhydroxyalkanoates (PHA) production by photoheterotrophic microbial consortia: effect of culture conditions over microbial population and biopolymer yield and composition. Eur Polym J 98:94–104. https://doi.org/10.1016/j.eurpolymj.2017.11.007

    Article  Google Scholar 

  23. Nielsen C, Rahman A, Rehman AU, Walsh MK, Miller CD (2017) Food waste conversion to microbial polyhydroxyalkanoates. Microb Biotechnol 10:1338–1352. https://doi.org/10.1111/1751-7915.12776

    Article  Google Scholar 

  24. Baird RB, Eaton AD, Rice EW (2001) Standard methods for the examination of water and wastewater. American Public Health Association. https://www.academia.edu/38769108/Standard_Methods_For_the_Examination_of_Water_and_Wastewater_23nd_edition. Accessed 15 May 2017

  25. Bhuwal AK, Singh G, Aggarwal NK, Goyal V, Yadab A (2013) Isolation and screening of polyhydroxyalkanoates producing bacteria from pulp, paper, and cardboard industry wastes. Int J Biomater 2013:1–10. https://doi.org/10.1155/2013/752821

    Article  Google Scholar 

  26. Rivas-Castillo AM, Valdez-Calderón A, Rojas-Avelizapa NG (2019) Culture medium to promote the production of biopolymers (in Spanish). Patent application no. 3895 submitted to the Ministry of Economy in 29 November 2019 for its presentation at the Mexican Institute of Industrial Property (IMPI)

  27. Amutha R, Kavusik T, Sudha A (2017) Analysis of bioactive compounds in citrus fruit peels. International Journal of Scientific Research and Review 6:19–27. https://www.researchgate.net/publication/323551324_ANALYSIS_OF_BIOACTIVE_COMPOUNDS_IN_CITRUS_FRUIT_PEELS. Accessed 14 June 2019

  28. Gusakov AV, Kondratyeva EG, Sinitsyn AP (2011) Comparison of two methods for assaying reducing sugars in the determination of carbohydrase activities. Int J Anal Chem 2011:283658–283654. https://doi.org/10.1155/2011/283658

    Article  Google Scholar 

  29. Jones DB (1941) Factors for converting percentages of nitrogen in foods and feeds into percentages of protein. US Department of Agriculture - Circular No 183. https://www.ars.usda.gov/ARSUserFiles/80400525/Data/Classics/cir183.pdf. Accessed 14 June 2019

  30. Mariotti F, Tomé D, Mirand PP (2008) Converting nitrogen into protein-beyond 6.25 and Jones Factors. Crit Rev Food Sci Nutr 48:177–184. https://doi.org/10.1080/10408390701279749

    Article  Google Scholar 

  31. Hungund B, Shyama VS, Patwhardan P, Saleh AM (2013) Production of polyhydroxyalkanoate from Paenibacillus durus BV-1 isolated from oil mill soil. J Microb Biochem Technol 5:13–17. https://doi.org/10.4172/1948-5948.1000092

    Article  Google Scholar 

  32. Sánchez-Moreno SA, Marín-Montoya MA, Mora-Martínez AL, Yepes-Pérez MS (2012) Identification of bacteria that produce polyhydroxyalcanoates (PHAs) in soils contaminated with residues of fique (in Spanish). Revista Colombiana de Biotecnología 16:89-100. https://revistas.unal.edu.co/ index.php/biotecnología/article/view/37286/39379. Accessed 26 May 2019

  33. Thapa C, Shakya P, Shrestha R, Pal S, Manandhar P (2018) Isolation of polyhydroxybutyrate (PHB) producing bacteria, optimization of culture conditions for PHB production, extraction and characterization of PHB. Nepal Journal of Biotechnology 6:62–68. https://www.researchgate.net/publication/330403719_Isolation_of_Polyhydroxybutyrate_PHB_Producing_Bacteria_Optimization_of_Culture_Conditions_for_PHB_production_Extraction_and_Characterization_of_PHB. Accessed 5 August 2020

  34. Kaur G, Roy I (2015) Strategies for large-scale production of polyhydroxyalkanoates. Chem Biochem Eng Q 29:157–172. https://doi.org/10.15255/CABEQ.2014.2255

    Article  Google Scholar 

  35. Pincus DH (2005) Microbial identification using the bioMériueux VITEK® 2 system. In: Miller MJ (ed) Encyclopedia of rapid microbiological methods. PDA, Barcelona, pp. 1–32

  36. Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York

    Google Scholar 

  37. Cutting SM, Vander Horn PB (1990) Genetic analysis. In: Harwood CR, Cutting SM (eds) Molecular biological methods for Bacillus. John Wiley & Sons, Sussex, pp 27–74

    Google Scholar 

  38. Ratul S, Farrance CE, Verghese B, Hong S, Donofrio RS (2013) Klebsiella michiganensis sp. nov., a new bacterium isolated from a tooth brush holder. Curr Microbiol 66:72–78. https://doi.org/10.1007/s00284-012-0245-x

    Article  Google Scholar 

  39. Shakib P, Kalani MT, Ramazanzadeh R, Ahmadi A, Rouhi S (2018) Molecular detection of virulence genes in Klebsiella pneumoniae clinical isolates from Kurdistan Province, Iran. Biomedical Research and Therapy 5:2581–2589. http://www.bmrat.org/index.php/BMRAT/article/view/467/919. Accessed 5 June 2018

  40. Ben M, Mato T, Lopez A, Vila M, Kennes C, Veiga MC (2011) Bioplastic production using wood mill effluents as feedstock. Water Sci Technol 63:1196–1202. https://doi.org/10.2166/wst.2011.358

    Article  Google Scholar 

  41. Al-Rowaihi IS, Paillier A, Rasul S, Karan R, Grötzinger SW, Takanabe K, Eppinger J (2018) Poly (3-hydroxybutyrate) production in an integrated electromicrobial setup: investigation under stress inducing conditions. PLoS One 13:1–13. https://doi.org/10.1371/journal.pone.0196079

    Article  Google Scholar 

  42. Toczyłowska-Mamińska R (2017) Limits and perspectives of pulp and paper industry wastewater treatment–a review. Renew Sust Energ Rev 78:764–772. https://doi.org/10.1016/j.rser.2017.05.021

    Article  Google Scholar 

  43. Ghribi M, Meddeb-Mouelhi F, Beauregard M (2016) Microbial diversity in various types of paper mill sludge: identification of enzyme activities with potential industrial applications. Springerplus 5:1–14. https://doi.org/10.1186/s40064-016-3147-8

    Article  Google Scholar 

  44. Karigar CS, Rao SS (2011) Role of microbial enzymes in the bioremediation of pollutants: a review. Enzyme Res 2011:1–11. https://doi.org/10.4061/2011/805187

    Article  Google Scholar 

  45. Singh P, Parmar N (2011) Isolation and characterization of two novel polyhydroxybutyrate (PHB)-producing bacteria. Afr J Biotechnol 10:4907–4919. Accessed 7 November 2017

  46. Flores-Vásquez ADP, Idrogo E-III, Carreño-Farfán CR (2018) Yield of polyhydroxyalcanoates (PHA) in halophilic microorganisms isolated from salt flats (in Spanish). Revista Peruana de Biología 25:153–160. https://doi.org/10.15381/rpb.v25i2.14249

    Article  Google Scholar 

  47. Ashby RD, Solaiman DKY, Strahan GD (2011) Efficient utilization of crude glycerol as fermentation substrate in the synthesis of poly (3-hydroxybutyrate) biopolymers. J Am Oil Chem Soc 88:949–959. https://doi.org/10.1007/s11746-011-1755-6

    Article  Google Scholar 

  48. Silva AF, Taciro MK, Michelin-Ramos ME, Carter JM, Pradella JGC, Gomez JGC (2004) Poly-3-hydroxybutyrate (P3HB) production by bacteria from xylose, glucose and sugarcane bagasse hydrolysate. J Ind Microbiol Biotechnol 31:245–254. https://doi.org/10.1007/s10295-004-0136-7

    Article  Google Scholar 

  49. Zhu C, Nomura CT, Perrotta JA, Stipanovic AJ, Nakas JP (2010) Production and characterization of poly-3-hydroxybutyrate from biodiesel-glycerol by Burkholderia cepacia ATCC 17759. Biotechnol Prog 26:424–430. https://doi.org/10.1002/btpr.355

    Article  Google Scholar 

  50. Li R, Jiang Y, Wang X, Yang J, Gao Y, Zi X, Zhang X, Gao H, Hu N (2013) Psychrotrophic Pseudomonas mandelii CBS-1 produces high levels of poly-β-hydroxybutyrate. Springerplus 2:1–7. https://doi.org/10.1186/2193-1801-2-335

    Article  Google Scholar 

  51. Oliveira FC, Dias ML, Castilho LR, Freire FMG (2007) Characterization of poly (3-hydroxybutyrate) produced by Cupriavidus necator in solid-state fermentation. Bioresour Technol 98:633–638. https://doi.org/10.1016/j.biortech.2006.02.022

    Article  Google Scholar 

  52. Kiran GS, Lipton AN, Anitha SPK, Cruz-Suárez LE, Arasu MV, Choi KC, Selvin J, Al-Dhabi NA (2014) Antiadhesive activity of poly-hydroxy butyrate biopolymer from a marine Brevibacterium casei MSI04 against shrimp pathogenic vibrios. Microb Cell Factories 13:1–12. https://doi.org/10.1186/s12934-014-0114-3

    Article  Google Scholar 

  53. Mohapatra S, Sarkar B, Samantaray DP, Daware A, Maity S, Pattnaik S, Bhattacharjee S (2017) Bioconversion of fish solid waste into PHB using Bacillus subtilis based submerged fermentation process. Environ Technol 38:3201–3208. https://doi.org/10.1080/09593330.2017.1291759

    Article  Google Scholar 

  54. Egli T (2015) Microbial growth and physiology: a call for better craftsmanship. Front Microbiol 6:1–12. https://doi.org/10.3389/fmicb.2015.00287

    Article  Google Scholar 

  55. Penkhrue W, Jendrossek D, Khanongnuch C, Pathom-aree W, Aizawa T, Behrens RL, Lumyong S (2020) Response surface method for polyhydroxybutyrate (PHB) bioplastic accumulation in Bacillus drentensis BP17 using pineapple peel. PLoS One 15:e0230443. https://doi.org/10.1371/journal.pone.0230443

    Article  Google Scholar 

  56. Batool N, Shakir HA, Qazi JI (2015) Comparative growth potential of different Bacillus species on fruit peels. Punjab University Journal of Zoology 30:25-29. http://pu.edu.pk/images/journal/zology/ PDF-FILES/6-Comparative%20growth%20potential%20of%20different%20Bacillus%20species%20 on% 20fruit%20peels.pdf. Accessed 12 June 2019

  57. Lyte M (1997) Induction of gram-negative bacterial growth by neurochemical containing banana (Musa x paradisiaca) extracts. FEMS Microbiol Lett 154:245–250. https://doi.org/10.1111/j.1574-6968.1997.tb12651.x

    Article  Google Scholar 

  58. Torrado AM, Cortés S, Salgado JM, Max B, Rodríguez N, Bibbins BP, Converti A, Domínguez JM (2011) Citric acid production from orange peel wastes by solid- state fermentation. Braz J Microbiol 42:394–409. https://doi.org/10.1590/S1517-83822011000100049

    Article  Google Scholar 

  59. Golden DA, Rhodehamel EJ, Kautter DA (1993) Growth of Salmonella spp. in cantaloupe, watermelon, and honeydew melons. J Food Prot 56:194–196. https://doi.org/10.4315/0362-028X-56.3.194

    Article  Google Scholar 

  60. Blin C, Passet V, Touchon M, Rocha EPC, Brisse S (2017) Metabolic diversity of the emerging pathogenic lineages of Klebsiella pneumoniae. Environ Microbiol 19:1881–1898. https://doi.org/10.1111/1462-2920.13689

    Article  Google Scholar 

  61. Lu X, Ji G, Zong H, Zhuge B (2016) The role of budABC on 1,3-propanediol and 2,3-butanediol production from glycerol in Klebsiella pneumoniae CICIM B0057. Bioengineered 7:439–444. https://doi.org/10.1080/21655979.2016.1169355

    Article  Google Scholar 

  62. Sun S, Zhang H, Lu S, Lai C, Liu H, Zhu H (2016) The metabolic flux regulation of Klebsiella pneumoniae based on quorum sensing system. Sci Rep 6:1–9. https://doi.org/10.1038/srep38725

    Article  Google Scholar 

  63. Afzal AM, Rasool MH, Waseem M, Aslam M (2017) Assessment of heavy metal tolerance and biosorptive potential of Klebsiella variicola isolated from industrial effluents. AMB Express 7:1–9. https://doi.org/10.1186/s13568-017-0482-2

    Article  Google Scholar 

  64. Aransiola EF, Ige OA, Ehinmitola EO, Lakoyun SK (2017) Heavy metals bioremediation potential of Klebsiella species isolated from diesel polluted soil. Afr J Bitechnol 16:1098–1105. https://doi.org/10.5897/AJB2016.15823

    Article  Google Scholar 

  65. Muñoz AJ, Espinola F, Ruiz E (2015) Removal of Pb(II) in a packed-bed column by a Klebsiella sp. 3S1 biofilm supported on porous ceramic Raschig rings. J Ind Eng Chem 40:118–127. https://doi.org/10.1016/j.jiec.2016.06.012

    Article  Google Scholar 

  66. Muñoz AJ, Espinola F, Ruiz ER (2017) Removal of heavy metals by Klebsiella sp. 3S1. Kinetics, equilibrium and interaction mechanisms of Zn (II) biosorption. J Chem Technol Biotechnol 93:1213–1511. https://doi.org/10.1002/jctb.5503

    Article  Google Scholar 

  67. Cardona-Echavarría AC, Mora-Martínez AL, Marín-Montoya M (2013) Molecular identification of bacteria that produce polyhydroxyalcanoates in dairy subproducts and sugar cane (in Spanish). Revista Facultad Nacional de Agronomía Medellín 66:7129-7140. https://revistas.unal.edu.co/index.php/refame/ article/view/41230/42790. Accessed 5 November 2018

  68. Fathima N, Krishnaswamy VG (2016) Optimization of poly-β-hydroxybutyrate production by halotolerant bacterial strains isolated from saline environment. Int J Bioassays 5.8:4775-4781. https://www.doi.org/2F10.21746%2Fijbio.2016.08.0011

  69. Favaro L, Basaglia M, Casella S (2018) Improving polyhydroxyalkanoate production from inexpensive carbon sources by genetic approaches: a review. Biofuels Bioprod Biorefin 13:208–227. https://doi.org/10.1002/bbb.1944

    Article  Google Scholar 

  70. Mitrea L, Vodnar DC (2019) Klebsiella pneumoniae—a useful pathogenic strain for biotechnological purposes: diols biosynthesis under controlled and uncontrolled pH levels. Pathogens 8:1–10. https://doi.org/10.3390/pathogens8040293

    Article  Google Scholar 

Download references

Acknowledgments

We deeply thank Prof. Gilberto José López de la Mora, Coordinator of the Scientific Illustration Program of the Universidad Tecnológica de la Zona Metropolitana del Valle de México, for his valuable assistance in the elaboration of the graphical abstract. Biochemical characterization of strain E22 was performed in Acosta Group—Laboratories, in Tizayuca, Hgo., Mexico.

Funding

This project was supported by the budget from the Annual Operational Program assigned to the Universidad Tecnológica de la Zona Metropolitana del Valle de México for Scientific, Technological, and Educational Research Projects, item numbers 251001 and 255001 for years 2017 and 2018.

Author information

Authors and Affiliations

  1. Universidad Tecnológica de la Zona Metropolitana del Valle de México, Blvd. Miguel Hidalgo y Costilla 5, Los Héroes de Tizayuca, 43816, Tizayuca, Hidalgo, Mexico

    A. Valdez-Calderón, M. A. Islas-Ponce, A. F. Angeles-Padilla & A. M. Rivas-Castillo

  2. Facultad de Ciencias Químicas, Universidad Juárez del estado de Durango, Av. Veterinaria S/N, Circuito Universitario, Valle del Sur, 34120, Durango, Mexico

    M. Barraza-Salas & M. A. Islas-Ponce

  3. División de Químico-Biológicas, Universidad Tecnológica de Tecámac, Km 37.5 Carretera Federal México-Pachuca, Colonia Sierra Hermosa, 55740, Tecámac, Estado de México, Mexico

    M. Quezada-Cruz & A. Garrido-Hernández

  4. Escuela de Ciencias de la Salud, Universidad del Valle de México, Campus Zapopan, Periférico Poniente 7900, Col. Jardines de Collí, 45010, Zapopan, Jalisco, Mexico

    S. Carrillo-Ibarra

  5. Grupo de Propiedades Ópticas de la Materia, Centro de Investigaciones en Óptica, A. P. 1-948, 37000, León, Guanajuato, Mexico

    M. Rodríguez

  6. Centro de Investigación en Ciencia Aplicada y Tecnología Avanzada del IPN, Cerro Blanco 141, Col. Colinas del Cimatario, 76090, Querétaro, Mexico

    N. G. Rojas-Avelizapa

Authors
  1. A. Valdez-Calderón
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Barraza-Salas
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Quezada-Cruz
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. A. Islas-Ponce
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. F. Angeles-Padilla
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. S. Carrillo-Ibarra
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. M. Rodríguez
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. N. G. Rojas-Avelizapa
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. A. Garrido-Hernández
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. A. M. Rivas-Castillo
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. M. Rivas-Castillo.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary information

ESM 1

(DOCX 121 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Valdez-Calderón, A., Barraza-Salas, M., Quezada-Cruz, M. et al. Production of polyhydroxybutyrate (PHB) by a novel Klebsiella pneumoniae strain using low-cost media from fruit peel residues. Biomass Conv. Bioref. 12, 4925–4938 (2022). https://doi.org/10.1007/s13399-020-01147-5

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13399-020-01147-5

Keywords

Search

Navigation