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Klebsiella michiganensis sp. nov., A New Bacterium Isolated from a Tooth Brush Holder

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Abstract

Isolate W14T recovered from a household tooth brush holder was found to be gram-negative, a facultative anaerobic, non-motile, capsulated, and a non-endospore-forming straight rod. Based on phylogenetic analysis with 16S rRNA gene sequence, isolate W14T was affiliated to the genus Klebsiella. The closest phylogenetic relative was K. oxytoca with 99 % similarity in the 16S rRNA gene sequence. The major whole-cell fatty acids were C16:0 (31.23 %), C18:1ω6c/C18:1ω7c (21.10 %), and C16:1ω7c/C16:1ω6c (19.05 %). The sequence similarities of isolate W14T based on rpoB, gyrA, and gyrB were 97, 98, and 98 % with K. oxytoca, and 97, 93, and 90 % with K. mobilis (=Enterobacter aerogenes), respectively. The ribotyping pattern showed a 0.46 similarity with K. oxytoca ATCC 13182T and 0.24 with K. mobilis ATCC 13048T. The DNA G+C content of isolate W14T was 54.6 mol%. The DNA–DNA relatedness was 55.7 % with K. oxytoca ATCC 13182T. Using the identification technology of MALDI-TOF mass spectrometry, the top matches for this isolate were K. oxytoca ATCC 13182T (Match Factor Score 1.998) and K. mobilis (Score 1.797). On the basis of phenotypic, biochemical, chemotaxonomic, and molecular studies, isolate W14T could be differentiated from other members of the genus Klebsiella including K. mobilis. Therefore, it is proposed that isolate W14T (=ATCC BAA-2403T=DSM 25444T) should be classified as the type strain of a novel species of the genus Klebsiella, K. michiganensis sp. nov.

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Acknowledgments

The authors would like to extend their sincere thanks to NSF International and Accugenix Inc. for providing funding and facilities for the project and also to Jodie Lee of Bacteriology at American Type Culture Collection (ATCC), Virginia, USA for excellent technical support.

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Authors and Affiliations

  1. Microbiology Division, NSF International, 789 Dixboro Road, Ann Arbor, MI, 48105, USA

    Ratul Saha & Robert S. Donofrio

  2. Accugenix Inc., 223 Lake Drive, Newark, DE, 19702, USA

    Christine E. Farrance, Bindhu Verghese & Sunhee Hong

Authors
  1. Ratul Saha
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  2. Christine E. Farrance
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  3. Bindhu Verghese
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  4. Sunhee Hong
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  5. Robert S. Donofrio
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Corresponding author

Correspondence to Ratul Saha.

Additional information

The GenBank accession numbers for the 16S rRNA gene, rpoB, gyrB, and the gyrA nucleotide sequences for strain W14T are JQ070300, JQ269337, JQ284304, and JQ990329.

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Saha, R., Farrance, C.E., Verghese, B. et al. Klebsiella michiganensis sp. nov., A New Bacterium Isolated from a Tooth Brush Holder. Curr Microbiol 66, 72–78 (2013). https://doi.org/10.1007/s00284-012-0245-x

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  • DOI: https://doi.org/10.1007/s00284-012-0245-x

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