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Comparative transcriptome profiling of freezing stress responsiveness in two contrasting Chinese cabbage genotypes, Chiifu and Kenshin

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Abstract

Freezing stress is a major factor affecting plant growth, crop productivity, and the geographical distribution of plants. To identify freezing-responsive genes in Brassica rapa, we analyzed transcriptome profiles of two contrasting inbred lines with different geographic origins, Chiifu and Kenshin, in control and −4 °C-treated leaves. A total of 3,301 genes were differentially expressed between Chiifu and Kenshin upon freezing treatment. Among these, 67 and 1,633 genes were specifically expressed in Chiifu and Kenshin, respectively. An ortholog (BrTPP1) of Arabidopsis trehalose-6-phosphate phosphatase 1 (TPP1) was specifically and highly induced in Chiifu by freezing treatment. However, most cold-responsive genes, including CBF pathway-related genes, showed similar patterns of expression between Chiifu and Kenshin. Many genes involved in stress responses (i.e., to freezing temperatures) were intrinsically and specifically expressed in Chiifu, which is tolerant of freezing temperatures. The results suggest that the CBF pathway is not the main factor conferring freezing tolerance to Chiifu, but genes that are expressed prior to cold acclimation, along with other regulatory genes, may play important roles in freezing tolerance.

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Acknowledgments

This work was supported by a Grant from the Next-Generation BioGreen 21 Program (the Next-Generation Genomics Center no. PJ008118 and Plant Molecular Breeding Center no. PJ009085), Rural Development Administration, Republic of Korea.

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The authors declare no conflict of interest.

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Authors and Affiliations

  1. Department of Biology, College of Biological Sciences, Chungnam National University, Daejeon, 305-764, Korea

    Xiangshu Dong & Yoonkang Hur

  2. Department of Horticulture, Chungnam National University, Daejeon, 305-764, Korea

    Su-Bin Im & Yong-Pyo Lim

  3. Department of Horticulture, Sunchon National University, Jeonnam, Suncheon-si, 540-742, Korea

    Ill-Sup Nou

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  1. Xiangshu Dong
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Correspondence to Yoonkang Hur.

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Dong, X., Im, SB., Lim, YP. et al. Comparative transcriptome profiling of freezing stress responsiveness in two contrasting Chinese cabbage genotypes, Chiifu and Kenshin. Genes Genom 36, 215–227 (2014). https://doi.org/10.1007/s13258-013-0160-y

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