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Stress inducible cytosolic ascorbate peroxidase (Ahcapx) from Arachis hypogaea cell lines confers salinity and drought stress tolerance in transgenic tobacco

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Abstract

Ascorbate peroxidase regulates toxic levels of hydrogen peroxide and catalyzes the reduction of H2O2 to water. A cDNA encoding cytosolic ascorbate peroxidase (Ahcapx) was isolated from the salt tolerant (ST) cell lines of Arachis hypogaea, that showed higher transcript level and 4.5 times higher total APX enzyme activity in the ST lines as compared to salt sensitive lines (SS). The transgenic tobacco plants overexpressing the Ahcapx gene were salinity and drought tolerant as compared to wild type (WT) plants. The transgenic plants had higher chlorophyll content and displayed enhanced germination rate under the abiotic stresses. In addition they also exhibited better photosynthetic efficiency, lower membrane damage and relatively higher values of ascorbate peroxidase activity under stress conditions. These results functionally validate a potential role of Ahcapx, as an important scavenger of reactive oxygen species useful in conferring stress tolerance in plants.

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Acknowledgments

UPOE Project from JNU, UGC, CSIR, DST-FIST and DST-PURSE is acknowledged for the funding. We thank Dr MK Reddy for the kind gift of Arachis hypogaea library and APX probe. Groundnut Research Institute, Junagarh, is acknowledged for providing the seeds (Var JL-24) for carrying out the investigation.

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    Authors and Affiliations

    1. School of Life Science, Jawaharlal Nehru University, New Delhi, 110067, India

      Divya Chavhan Shrivastava, Arun Vincent Kisku, Mukesh Saxena & Neera Bhalla Sarin

    2. Department of Botany, University of Delhi, Delhi, 110007, India

      Renu Deswal

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    1. Divya Chavhan Shrivastava
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    Correspondence to Neera Bhalla Sarin.

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    Divya Chavhan Shrivastava and Arun Vincent Kisku contributed equally to this work.

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    Shrivastava, D.C., Kisku, A.V., Saxena, M. et al. Stress inducible cytosolic ascorbate peroxidase (Ahcapx) from Arachis hypogaea cell lines confers salinity and drought stress tolerance in transgenic tobacco. Nucleus 58, 3–13 (2015). https://doi.org/10.1007/s13237-015-0134-3

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    • DOI: https://doi.org/10.1007/s13237-015-0134-3

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