Summary
To determine NaCl effects on callus growth and antioxidant activity, callus of a salt-tolerant and a salt-sensitive cultivar of cotton was grown on media amended with 0, 75, and 150 mM NaCl. Callus of the salt-tolerant cultivar, Acala 1517-8 8, grown at 150 mM NaCl, showed significant increases in superoxide dismutase, catalase, ascorbate peroxidase, peroxidase and glutathione reductase activities compared to callus tissue grown at 0 mM NaCl. In contrast, callus tissue of the salt-sensitive cultivar, Deltapine 50, grown at 0, 75, and 150 mM NaCl, showed no difference in the activities of these enzymes. At the 150 mM NaCl treatment, peroxidase was the only antioxidant enzyme from Deltapine 50 with an activity as high as that observed in Acala 1517-88. The NaCl-induced increase in the activity of these enzymes in Acala 1517-88 indicates that callus tissue from the more salt-tolerant cultivar has a higher capacity for scavenging and dismutating superoxide, an increased ability to decompose H2O2, and a more active ascorbate-glutathione cycle when grown on media amended with NaCl.
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Anderson JV, Chevone BI, Hess JL (1992) Seasonal variation in the antioxidant system of eastern white pine needles. Plant Physiol 98:501–508
Beers RF, Sizer I (1952) A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem 195:133
Cakmak I, Marschner H (1992) Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase, and glutathione reductase in bean leaves. Plant Physiol 98:1222–1227
Chang H, Siegel BZ, Siegel SM (1984) Salinity induced changes in isoperoxidase in taro, Colocasia esculenta. Phytochem 23:233–235
Chen G, Asada K (1989) Ascorbate peroxidase in tea leaves: occurrence of two isozymes and the differences in their enzymatic and molecular properties. Plant Cell Physiol 30:987–998
Davies KJA (1987) Protein damage and degradation by oxygen radicals I. General aspects. J Biol Chem 262:9895–9901
Dhindsa RS, Matowe W (1981) Draught tolerance in two mosses: correlated with enzymatic defense against lipid peroxidation. J Exp Bot 32:79–91
Finazzi-Agro A., Di Giulio A, Amicosante G, Crifo C (1986) Photohemolysis of erythrocytes enriched with superoxide dismutase, catalase, and glutathione peroxidase. Photochem Photobiol 43:409–412
Forman HJ, Fridovich I (1973) Superoxide dismutase: A comparison of rate constants. Arch Biochem Biophys 158:396–400
Foster JG, Hess JL (1980) Responses of superoxide dismutase and glutathione reductase activities in cotton leaf tissue exposed to an atmosphere enriched in oxygen. Plant Physiol 66: 482–487
Foyer CH, Lelandais M, Edwards EA, Mullineaux PM (1991) The role of ascorbate in plants, interactions with photosynthesis, and regulatory significance. In E. Pell, K Steffen, eds, Active Oxygen/Oxidative Stress and Plant Metabolism. Am Soc Plant Physiol, pp 131–144
Fridovich I (1986) Biological effects of the superoxide radical. Arch Biochem Biophys 247:1–11
Gamborg OL (1978) Plant Tissue Culture. TA Thorpe, ed. Academic Press, Orlando FL. p 36
Gossett DR, Millhollon EP, Caldwell WD, Mundy S. 1991. Isozyme variation among salt tolerant and salt sensitive varieties of cotton. Proceed. Beltwide Cotton Res. Conf. National Cotton Council, Memphis, TN, pp 556–559
Gossett DR, Lucas MC, Millhollon EP, Caldwell WD, Barclay A (1992) Antioxidant status in salt stressed cotton. Proceed. Beltwide Cotton Res. Conf. National Cotton Council, Memphis, TN, pp 1036–1039
Gossett, DR, Millhollon EP, Lucas MC, and Marney MM (1993) Antioxidant Enzymes and Salt Stress in Cotton. Beltwide Res. Conf. National Cotton Council. Memphis, TN, pp. 1262–1266
Halliwell B, Gutteridge JMC (1985) Free Radicals in Biology and Medicine. Clarendon Press, Oxford, p 29
Harper DB, Harvey BMR (1978) Mechanisms of paraquat tolerance in perennial ryegrass II. Role of superoxide dismutase, catalase, and peroxidase. Plant Cell Environ 1:211–215
Helmerhorst E, Stokes GB (1980) Microcentrifuge desalting: A rapid quantitative approach for desalting small amounts of protein. Ann Biochem 104:103–135
Hossain M, Nakano K, Asada K (1984) Monodehydroascorbate reductase in spinach chloroplasts and its participation in the regeneration of ascorbate for scavenging hydrogen peroxide. Plant Cell Physiol 61:385–395
Hurkman WJ, Fornari CS, Tanaka CK (1989) A comparison of the effects of salt on polypeptides and translatable mRNAs in roots of a salt-tolerant and salt-sensitive cultivar of barley. Plant Physiol 90:1444–1456
Imlay JA, Linn S (1988) DNA damage and oxygen radical toxicity. Science 240:1302–1309
Lauchli L, Kent LM, Turner JC (1981) Physiological responses of cotton genotypes to salinity. Proceed. Beltwide Cotton Res. Conf., National Cotton Council, Memphis, TN, p 40
Monk LS, Davies HV (1989) Antioxidant status of the potato tuber and Ca2+ deficiency as a physiological stress. Physiologia Plant 75:411–416
Murashige T and Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultue. Plant Physiol. 15:473–497
Nickel RS, Cunningham BA (1969) Improved peroxidase assay method using leuco 2,3′,6 trichloroindophenol and application to comparative measurements of peroxidase catalysis. Ann Biochem 27:292–299
Polle A, Chakrabarti K, Chakrabarti S, Seifert F, Schramel P, Rennenberg H (1992) Antioxidants and manganese deficiency in needles of norway spruce (Picea abies L.) trees. Plant Physiol 99:1084–1089
Ramagopal S (1987) Salinity stress induced tissue specific proteins in barley. Plant Physiol 84:324–331
Schaedle M, Bassham JA (1977) Chloroplast glutathione reductase. Plant Physiol 59:1011–1012
Smith MK and JA McComb (1981a) Effects of NaCl on the growth of whole plants and their corresponding callus cultures. Aust. J. Plant Physiol. 8:267–275
Smith MK and McComb JA (1981b) Use of callus cultures to detect NaCl tolerance in cultivars of three pasture legumes. Aust. J. Plant Physiol. 8:437–442
Spychalla JP, Desborough SL (1990) Superoxide dismutase, catalase, and alpha-tocopherol content of stored potato tubers. Plant Physiol 94:1214–1218
Stewart JM and Hsu CL (1977) In-ovulo embryo culture and seedling development of cotton (Gossypium hirsutum L). Planta 137:113–117
Trolinder NL and Goodin JR (1987) Somatic embryogenesis and plant regeneration in Gossypium hirstum L. Plant Cell Rep. 6:231–234
Wise RR, Naylor AW (1987) Chiling-enhanced photooxidation: Evidence for the role of singlet oxygen and endogenous antioxidants. Plant Physiol 83:278–282
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Gossett, D.R., Millhollon, E.P., Lucas, M.C. et al. The effects of NaCl on antioxidant enzyme activities in callus tissue of salt-tolerant and salt-sensitive cotton cultivars (Gossypium hirsutum L.). Plant Cell Reports 13, 498–503 (1994). https://doi.org/10.1007/BF00232944
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DOI: https://doi.org/10.1007/BF00232944