Abstract
The development of biofriendly and economical alternatives to chemical pesticides is a globally important scientific challenge. In this work, Karanja-based media conditions were optimized for obtaining high production of biomass and spores of a biocontrol agent, the entomopathogenic fungus Paecilomyces lilacinus 6029, using a two-step statistical approach coupled with rigorous experimentation. In the first step, non-edible Karanja cake was screened out as a major substrate from other oil cakes. In the second step, biomass production was maximized by applying response surface methodology to experimental variations in key physico-chemical factors: carbon/nitrogen (C/N) ratio and pH. This approach eventually predicted a maximum biomass production of 10.559 g/l with a medium having a C/N ratio of 35.88 and pH 5.9. An experimental production of 10.529 g/l biomass was obtained. The remarkable agreement between the predicted and the experimentally obtained biomass confirm the validity of the approach utilized to maximize production of P. lilacinus.
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Acknowledgment
The authors gratefully acknowledge financial support provided by the National Oilseed and Vegetable Oil Development (NOVOD) Board, Gurgaon, India, for carrying out the research.
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Sharma, A., Sharma, S., Mittal, A. et al. Statistical optimization of growth media for Paecilomyces lilacinus 6029 using non-edible oil cakes. Ann Microbiol 64, 515–520 (2014). https://doi.org/10.1007/s13213-013-0683-0
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DOI: https://doi.org/10.1007/s13213-013-0683-0