Abstract
An endo-β-1,3-glucanase with a molecular mass of 33.2 kDa was purified from Mitsuaria chitosanitabida H12 for the first time. The optimal temperature and pH were at 52°C and pH 5.2, respectively, with laminaran as the substrate. The K m and V max were 1.145 mg/ml and 0.634 μmol min−1 ml−1, respectively, with the initial velocity solved by hyperbolic equation fitting and seeking the first-order derivative at its origin (0) point. After optimization, 20 and 50 mg/ml, 1,3-β-d-glucooligosaccharides of different lengths could be produced from yeast β-glucan with the enzyme.
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Acknowledgments
This work was supported by the National High Technology Research and Development Program of China (863 Program) (2006AA10Z342), National Natural Science Foundation of China (30870021) and Sci-tech Development Project of Shandong Province (2007GG30002020).
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Duan, F., Lu, X. Enzymatic properties and kinetics of an endo-β-1,3-glucanase of Mitsuaria chitosanitabida H12 and preparation of 1,3-β-d-glucooligosaccharides from yeast β-glucan. Ann Microbiol 62, 307–312 (2012). https://doi.org/10.1007/s13213-011-0263-0
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DOI: https://doi.org/10.1007/s13213-011-0263-0