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Anti-apoptotic Activity of Laminarin Polysaccharides and their Enzymatically Hydrolyzed Oligosaccharides from Laminaria japonica

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Abstract

Laminarin polysaccharides (LP1) were prepared from Laminaria japonica, a marine brown alga with potential biological activities, by hot water extraction, ultrafiltration and gel chromatography; the molecular weights of the LP1s were between 5 and 10 kDa. Laminarin oligosaccharides (LO) derived by hydrolyzing LP1 with an endo-β-(1→3)-glucanase from Bacillus circulans were mainly di- and penta-oligosaccharides. Treatment of mouse thymocytes with LO or LP1 (1–4 mg ml−1) suppressed apoptotic death around 3- or 2-fold and extended cell survival in culture at a rate of about 30 or 20%. A mouse cDNA microarray showing the genes coding for immune response proteins were induced and apoptotic cell death proteins were reduced significantly by LO provided preliminary information regarding the immunomodulatory mechanism of LO. These results suggest that laminarin oligosaccharides and polysaccharides can be utilized to develop new immunopotentiating substances and functional alternative medicines.

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Authors and Affiliations

  1. Department of Biomedical Laboratory Science and Biohealth Products Research Center, Inje University, Gimhae, 621-749, Republic of Korea

    Ki-Hoon Kim, Yea-Woon Kim & Dong Seok Lee

  2. Department of Life Science, Hoseo University, Asan, 336-795, Republic of Korea

    Han Bok Kim

  3. Department of Chemistry and Biohealth Products Research Center, Inje University, Gimhae, 621-749, Republic of Korea

    Burm Jong Lee

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  1. Ki-Hoon Kim
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  2. Yea-Woon Kim
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  3. Han Bok Kim
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  5. Dong Seok Lee
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Correspondence to Dong Seok Lee.

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Revisions requested 26 October 2005; Revisions received 19 December 2005

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Kim, KH., Kim, YW., Kim, H.B. et al. Anti-apoptotic Activity of Laminarin Polysaccharides and their Enzymatically Hydrolyzed Oligosaccharides from Laminaria japonica. Biotechnol Lett 28, 439–446 (2006). https://doi.org/10.1007/s10529-005-6177-9

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  • DOI: https://doi.org/10.1007/s10529-005-6177-9

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