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Temporal analysis of molecular changes in shrimp (Penaeus vannamei) tissues with respect to white spot disease

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Abstract

In spite of lot of attention and significant research efforts, White spot disease (WSD) is the major cause for shrimp mortality in aquaculture industry. This is due to the limited understanding in White Spot Syndrome Virus (WSSV) pathogenesis. To understand shrimp molecular responses towards WSSV infection, proteome and protease profiles of various shrimp tissues (gill, muscle, gut and hepatopancreas) were studied at different time-intervals post-infection (pi) using SDS-PAGE analysis and In-gel gelatin zymography, respectively. Expression of new proteins along with up-regulation, down-regulation and varied expression of many host proteins were observed. These variations were observed as early as 6 h pi and the maximum variations were observed at the time-intervals 6 h pi and 12 h pi representing the early stage of infection. Protease profile analysis had revealed that most of the host proteases were down-regulated during WSSV infection. Among the tested shrimp tissues, gill, gut and hepatopancreas are the most affected due to WSSV infection, while muscle is the least affected one with minimum proteolytic activity, whereas hepatopancreas is highly enriched with active proteases. These results suggest that during WSSV infection, both protein and protease profiles of shrimp tissues gets drastically altered and down-regulation of the host proteases is the major step in WSSV-pathogenesis. These observations are significant for intervening with the early stages and delaying the morbidity and mortality, so that shrimps could be harvested at profitable incubation time and reduce the impact on shrimp aquaculture industry.

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Acknowledgments

We thank (the late) Dr V. Murugan, Centre for Biotechnology, Anna University for his initial contributions and we dedicate the work to his memory. The financial support of the DBT for InNoVacc project (Grant – BT/AAQ/Indo-Norway/183196/2007) is gratefully acknowledged. The investigators of InNoVacc project are acknowledged for their valuable support. We thank Mr. S. Kumar, Research Scholar, Centre for Biotechnology for his help in procuring the shrimps and in the experimentation. PAK thanks DBT for the Senior Research Fellowship.

Conflict of interest

The authors declare that they have no competing interests.

Authors contribution

PAK and KS participated in design, interpretation of data and revision of manuscript. PAK performed the shrimp study and tissue analysis, and drafted the manuscript.

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Authors and Affiliations

  1. Centre for Biotechnology, Anna University, Chennai, 600 025, Tamil Nadu, India

    Anand Kumar Pemula & Sankaran Krishnan

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  1. Anand Kumar Pemula
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  2. Sankaran Krishnan
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Correspondence to Sankaran Krishnan.

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Pemula, A.K., Krishnan, S. Temporal analysis of molecular changes in shrimp (Penaeus vannamei) tissues with respect to white spot disease. J Food Sci Technol 52, 7236–7244 (2015). https://doi.org/10.1007/s13197-015-1866-4

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  • DOI: https://doi.org/10.1007/s13197-015-1866-4

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