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Differential proteome profile of gill and spleen in three pathogen-infected Paralichthys olivaceus

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Abstract

Background

Olive flounder (Paralichthys olivaceus) is one of the major cultured fish species in Asia including Korea. However, the mass mortality of olive flounder caused by various pathogens leads to huge economic loss. The pathogens that lead to fish mortality include parasites, bacteria, and viruses that can cause various kinds of diseases.

Objective

The purpose of this study was to investigate the protein expression patterns in the gills and spleens of olive flounder after artificial infection. We hypothesized that proteomics levels in gills and spleen may be differentially expressed depending on infectious agents.

Methods

To investigate the expression pattern of proteins in gills and spleens, olive flounders were experimentally infected with VHSV (virus), S. parauberis (bacteria), or M. avidus (pathogenic ciliate). Proteins were extracted from the gills and spleens of infected olive flounder. We used 2-DE analysis with LC–MS/MS to investigate proteome changes in infected olive flounders.

Results

The results of the LC–MS/MS analyses showed different protein expression profiles depending on pathogenic sources and target organs. Proteins related to cytoskeletal structure like keratin, calmodulin and actin were mostly expressed in the infected gills. Proteins involved in the metabolism pathway like glycolysis were expressed mainly in the spleens. The protein profiles of S. parauberis and VHSV infection groups had many similarities, but the profile of the M. avidus infection group was greatly different in the gill and spleen.

Conclusion

Our results indicate that measures according to the characteristics of each pathogen are necessary for disease prevention and treatment of farmed fish.

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Data availability statement

The raw processed data required to reproduce these findings cannot be shared at this time as the data also forms part of an ongoing study.

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Acknowledgement

This research was a part of a project titled “Omics based on fishery disease control technology development and industrialization (20150242),” funded by the Ministry of Oceans and Fisheries, Korea.

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Authors and Affiliations

  1. Department of Parasitology and Genetics, Institute for Medical Science, Kosin University College of Medicine, Busan, Republic of Korea

    A-Reum Lee, Hyunsu Kim, Kyung-Yoon Jeon, Eun-Ji Ko, Mee Sun Ock & Hee-Jae Cha

  2. Pathology Research Division, National Institute of Fisheries Science, Busan, Republic of Korea

    Ahran Kim

  3. Department of Aquatic Life Medicine, College of Fisheries Science, Pukyong National University, 34, Amnam-dong, Seo-gu, Busan, 602-703, Republic of Korea

    Nameun Kim, HyeongJin Roh, Yoonhang Lee, Jiyeon Park & Do-Hyung Kim

  4. Department of Biochemistry, College of Oriental Medicine, Dongeui University, Busan, Republic of Korea

    Yung Hyun Choi

  5. Department of Chemistry, College of Natural Sciences, Pusan National University, Busan, Republic of Korea

    Suhkmann Kim

  6. Department of Biological Sciences, College of Natural Sciences, Pusan National University, Busan, Republic of Korea

    Heui-Soo Kim

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  1. A-Reum Lee
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Correspondence to Hee-Jae Cha.

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Lee, AR., Kim, H., Jeon, KY. et al. Differential proteome profile of gill and spleen in three pathogen-infected Paralichthys olivaceus. Genes Genom 43, 701–712 (2021). https://doi.org/10.1007/s13258-021-01097-w

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  • DOI: https://doi.org/10.1007/s13258-021-01097-w

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