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Probiotic Properties of Lactic Acid Bacteria Isolated from Water-Buffalo Mozzarella Cheese

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Abstract

This study evaluated the probiotic properties (stability at different pH values and bile salt concentration, auto-aggregation and co-aggregation, survival in the presence of antibiotics and commercial drugs, study of β-galactosidase production, evaluation of the presence of genes encoding MapA and Mub adhesion proteins and EF-Tu elongation factor, and the presence of genes encoding virulence factor) of four LAB strains (Lactobacillus casei SJRP35, Leuconostoc citreum SJRP44, Lactobacillus delbrueckii subsp. bulgaricus SJRP57 and Leuconostoc mesenteroides subsp. mesenteroides SJRP58) which produced antimicrobial substances (antimicrobial peptides). The strains survived the simulated GIT modeled in MRS broth, whole and skim milk. In addition, auto-aggregation and the cell surface hydrophobicity of all strains were high, and various degrees of co-aggregation were observed with indicator strains. All strains presented low resistance to several antibiotics and survived in the presence of commercial drugs. Only the strain SJRP44 did not produce the β-galactosidase enzyme. Moreover, the strain SJRP57 did not show the presence of any genes encoding virulence factors; however, the strain SJRP35 presented vancomycin resistance and adhesion of collagen genes, the strain SJRP44 harbored the ornithine decarboxylase gene and the strain SJRP58 generated positive results for aggregation substance and histidine decarboxylase genes. In conclusion, the strain SJRP57 was considered the best candidate as probiotic cultures for further in vivo studies and functional food products development.

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Acknowledgments

The authors are grateful to the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, Brazil, Projects No. 2010/09302-1 and 2011/11922-0), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Brazil) for the financial support, and to Dr. Elisabetta Tome from Universidad Central de Venezuela, Caracas, Venezuela, for providing Leuc. mesenteroides subsp. mesenteroides UCV10CET strain (CDCH Project No PG-03.7371.2008).

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The authors declare that there is no conflict of interest.

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Authors and Affiliations

  1. Department of Food Engineering and Technology, UNESP - Sao Paulo State University, Rua Cristóvão Colombo, 2265, Jardim Nazareth, São José do Rio Preto, 15.0540-000, Brazil

    Ana Beatriz Jeronymo-Ceneviva, Aline Teodoro de Paula, Luana Faria Silva & Ana Lúcia B. Penna

  2. Department of Food Science and Experimental Nutrition, Faculty of Pharmaceutical Sciences, USP - Sao Paulo University, São Paulo, Brazil

    Svetoslav Dimitrov Todorov & Bernadette Dora G. Mello Franco

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  1. Ana Beatriz Jeronymo-Ceneviva
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  2. Aline Teodoro de Paula
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  3. Luana Faria Silva
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  4. Svetoslav Dimitrov Todorov
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  5. Bernadette Dora G. Mello Franco
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  6. Ana Lúcia B. Penna
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Correspondence to Ana Lúcia B. Penna.

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Jeronymo-Ceneviva, A.B., de Paula, A.T., Silva, L.F. et al. Probiotic Properties of Lactic Acid Bacteria Isolated from Water-Buffalo Mozzarella Cheese. Probiotics & Antimicro. Prot. 6, 141–156 (2014). https://doi.org/10.1007/s12602-014-9166-2

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