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Adhesion of the probiotic strains Enterococcus mundtii ST4SA and Lactobacillus plantarum 423 to Caco-2 cells under conditions simulating the intestinal tract, and in the presence of antibiotics and anti-inflammatory medicaments

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Abstract

Adhesion of Enterococcus mundtii ST4SA and Lactobacillus plantarum 423 to Caco-2 (human carcinoma epithelial) cells was visualized by fluorescent staining. Both strains showed good adhesion compared to L. casei MB1, L. casei Shirota, L. johnsonii La1 and L. rhamnosus GG. No correlation was found between hydrophobicity, aggregation and adhesion to Caco-2 cells. Presence of antibiotics and anti-inflammatory medicaments reduced adhesion of bacterial strains to Caco-2 cells. Proteins sensitive to pepsin, trypsin and pronase are involved in the adhesion of E. mundtii ST4SA and L. plantarum 423 to Caco-2 cells. Adhesion of Listeria monocytogenes ScottA to Caco-2 cells was not prevented by E. mundtii ST4SA and L. plantarum 423. Cell-free culture supernatants of strains ST4SA and 423, containing the antimicrobial peptides plantaricin 423 and peptide ST4SA, prevented the invasion of L. monocytogenes ScottA into Caco-2 cells.

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Acknowledgments

This study was funded by Cipla Medpro (Pty) Ltd and the National Research Foundation (NRF) of South Africa.

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Authors and Affiliations

  1. Department of Microbiology, University of Stellenbosch, Stellenbosch, 7600, South Africa

    Marelize Botes, Carol A. van Reenen & Leon M. T. Dicks

  2. Department of Physiological Sciences, University of Stellenbosch, Stellenbosch, 7600, South Africa

    Ben Loos

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  1. Marelize Botes
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  2. Ben Loos
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  3. Carol A. van Reenen
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  4. Leon M. T. Dicks
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Correspondence to Leon M. T. Dicks.

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Communicated by Erko Stackebrandt.

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Botes, M., Loos, B., van Reenen, C.A. et al. Adhesion of the probiotic strains Enterococcus mundtii ST4SA and Lactobacillus plantarum 423 to Caco-2 cells under conditions simulating the intestinal tract, and in the presence of antibiotics and anti-inflammatory medicaments. Arch Microbiol 190, 573–584 (2008). https://doi.org/10.1007/s00203-008-0408-0

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  • DOI: https://doi.org/10.1007/s00203-008-0408-0

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