Abstract
Somatic embryos were induced from internodal segment derived callus of Oldenlandia umbellata L., in MS medium supplemented with different concentrations of 2,4-Dichlorophenoxy acetic acid (2,4-D). Initially calli were developed from internodes of microshoots inoculated in 2.5 µM NAA supplemented medium. Then calli were transferred to 2,4-D added medium for somatic embryogenesis. Nutritional stress coupled with higher concentration of 2,4-D triggered somatic embryogenesis. Nutritional stress was induced by culturing callus in a fixed amount of medium for a period up to 20 weeks without any external supply of nutrients. Addition of 2.5 µM 2,4-D gave 100% embryogenesis within 16 weeks of incubation. Callus mass bearing somatic embryos were transferred to germination medium facilitated production of in vitro plantlets. MS medium supplemented with 2.5 µM benzyl adenine and 0.5 µM α-naphthalene acetic acid produced 15.33 plants per culture within 4 weeks of culture. Somatic embryo germinated plants were then hardened and transferred to green house.
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References
Davletova S, Mészáros T, Miskolczi P, Oberschall A, Török K, Magyar Z, Dudits D, Deák M (2001) Auxin and heat shock activation of a novel member of the calmodulin like domain protein kinase gene family in cultured alfalfa cells. J Exp Bot 52:215–221
Dudits D, Györgyey J, Bögre L, Bako L (1995) Molecular biology of somatic embryogenesis. In: Thorpe TA (ed) In vitro embryogenesis in plants. Kluwer Academic Publishers, Dordrecht, pp 267–308
Duncan DB (1955) Multiple range and multiple F tests. Biometrics 11:1–42
Feher A (2005) Why somatic plant cells start to form embryos? In: Mujid A, Samaj J (eds) Somatic embryogenesis. Plant cell monographs. Springer, Berlin, pp 85–101
Jin F, Hu L, Yuan D, Xu J, Gao W, He L, Yang X, Zhang X (2014) Comparative transcriptome analysis between somatic embryos (SEs) and zygotic embryos in cotton: evidence for stress response functions in SE development. Plant Biotechnol J 12:161–173
Krishnan SRS, Siril EA (2015) Enhanced in vitro shootregeneration in Oldenlandia umbellata L. by using quercetin: anaturally occurring auxin-transport Inhibitor. P Natl Acad Sci India Sect B Biol Sci. doi:10.1007/s40011-015-0672-0
Law RD, Suttle JC (2005) Chromatin remodeling in plant cell culture: patterns of DNA methylation and histone H3w and H4 acetylation vary during growth of asynchronous potato cell suspensions. Plant Physiol Biochem 43:527–534
Lo Schiavo F, Pitto L, Giuliano G, Torti G, Nutironchi V, Marazziti D, Vergara R, Selli S, Terzi M (1989) DNA methylation of embryogenic carrot cell cultures and its variations ascaused by mutation, differentiation, hormones and hypomethylating drugs. Theor Appl Genet 77:325–331
Mahibalan S, Rao PC, Khan R, Basha A, Siddareddy R, Masubuti H, Fujimoto Y, Begum AS (2016) Cytotoxic constituents of Oldenlandia umbellata and isolation of a new symmetrical coumarin dimer. Med Chem Res. doi:10.1007/s00044-015-1500-z
Potters G, Pasternak T, Guisez Y, Palme KJ, Jansen M (2007) Stress-induced morphogenic responses: growing out of trouble? Trends Plant Sci 12:98–105
Rao GP, Bahadur B (1990) Somatic embryogenesis and plant regeneration in self-incompatible Oldenlandia umbellata L. (Rubiaceae). Phytomorphology 40:95–101
Siva R (2007) Status of natural dyes and dye yielding plants in India. Curr Sci 92:916–925
Siva R, Mudgal G, Rajesh D, Khan NF, Vijayakumar V, Rajasekaran C (2009a) Characterization of novel pH indicator of natural dye Oldenlandia umbellata L. Nat Prod Res 23:1210–1217
Siva R, Rajasekaran C, Mudgal G (2009b) Induction of somatic embryogenesis and organogenesis in Oldenlandia umbellata L., a dye-yielding medicinal plant. Plant Cell Tiss Org 98:205–211
Siva R, Mayes S, Behera SK, Rajasekaran C (2012) Anthraquinones dye production using root cultures of Oldenlandia umbellata L. Ind Crop Prod 37:415–419
SnedecorGW Cochran WG (1962) Statistical methods, vol 369. The Iowa State University Press, Iowa
Steward N, Ito M, Yamaguchi Y, Koizumu N, Sano H (2002) Periodic DNA methylation in maize nucleosomes and demethylation by environmental stress. J BiolChem 277:37741–37746
Su YH, Zhao XY, Liu YB, Zhang CL, O’Neill SD, Zhang XS (2009) Auxin-induced WUS expression is essential for embryonic stem cell renewal during somatic embryogenesis in Arabidopsis. Plant J 59:448–460
Talapatra S, Ghoshal N, Raychaudhuri SS (2014) Molecular characterization, modeling and expression analysis of a somatic embryogenesis receptor kinase (SERK) gene in Momordica charantia L., during somatic embryogenesis. Plant Cell Tiss Org 116:271–283
Terzi M (1989) DNA methylation of embryogenic carrot cell cultures and its variations as caused by mutation, differentiation, hormones and hypomethylating drugs. TheorAppl Genet 77:325–331
Williams L, Zhao J, Morozava N, Li Y, Avivi Y, Grafi G (2003) Chromatin reorganization accompanying cellular differentiation is associated with modifications of histone H3, redistribution of HP1, and activation of E2F-target genes. Dev Dyn 228:113–120
Yang X, Zhang X (2010) Regulation of somatic embryogenesis in higher plants. CrcCr Rev Plant Sci 29:36–57
Yang X, Zhang X, Yuan D, Jin F, Zhang Y, Xu J (2012) Transcript profiling reveals complex auxin signaling pathway and transcription regulation involved in dedifferentiation and redifferentiation during somatic embryogenesis in cotton. BMC Plant Biol 12:110–129
Zavattieri MA, Frederico AM, Lima M, Sabino R, Arnholdt-Schmitt B (2010) Induction of somatic embryogenesis as an example of stress-related plant reactions. Electron J Biotechnol 13:12–13
Zimmerman JL (1993) Somatic embryogenesis: a model for early development in higher plants. Plant Cell 5:1411–1423
Acknowledgements
The authors are thankful to Dr. Suharabeevy, Professor and Head, Department of Botany University of Kerala for facilities provided. SKSR thank University of Kerala, Thiruvananthapuram, India for granting University JRF (Ac E1B1/43700/2011 dt. 26/12/11).
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Krishnan, S.R.S., Siril, E.A. Auxin and nutritional stress coupled somatic embryogenesis in Oldenlandia umbellata L.. Physiol Mol Biol Plants 23, 471–475 (2017). https://doi.org/10.1007/s12298-017-0425-z
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DOI: https://doi.org/10.1007/s12298-017-0425-z