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Efficacy of RAPD, ISSR and DAMD markers in assessment of genetic variability and population structure of wild Musa acuminata colla

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Abstract

North east India is considered as one of the major biodiversity hotspots worldwide and centre of origin of several plant species including Musa. Musa acuminata Colla is known to be one of the wild progenitors of cultivated bananas and plantains. Three single primer based DNA marker techniques viz., random amplified polymorphic DNA (RAPD), inter simple sequence repeat (ISSR) and directed amplification of minisatellites DNA (DAMD) were used for diversity diagnostics among 25 genotypes of wild M. acuminata collected from Meghalaya province of north east India. A total of 58 primers (26-RAPD, 21-ISSR, and11-DAMD) yielded 451 DNA fragments, of which 395 (87.58 %) were found to be polymorphic in nature. The polymorphic information content (PIC) values were almost identical for each marker system. The resolving power of the marker system was found to be highest in RAPD (3.96) whereas ISSR resolved highest marker index (16.39) in the study. Selected amplicon data obtained through single primer amplification reactions were utilized for determination of diversity within and among the populations of M. acuminata. Nei’s genetic differentiation (Gst) value (0.451) indicated higher proportion of the genetic variation within the populations which is supported by the AMOVA analysis (88 %). The study provides insight into the efficacy of RAPD, ISSR and DAMD to analyse the genetic variation existing in the wild Musa germplasm, which can further be exploited for quality trait improvement and domestication of such important horticultural crops. The genetic diversity based population structure may shed light on the genetic basis of speciation and evolution of various species within the genus Musa.

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Acknowledgments

We thank the Head, Department of Biotechnology and Bioinformatics, NEHU, Shillong for providing the facilities and support to carry out this research. Sincere thanks are also accorded to Dr. C.S. Rao (St. Anthony’s College, Shillong, Meghalaya), Dr. Santosh K. Sharma, Dr. Shrawan Kumar and all members of the Plant Biotechnology Laboratory for their constant encouragement and support.

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  1. Department of Biotechnology & Bioinformatics, Plant Biotechnology Laboratory, North-Eastern Hill University, Shillong, 793022, Meghalaya, India

    Animos Lamare & Satyawada Rama Rao

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  1. Animos Lamare
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  2. Satyawada Rama Rao
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Correspondence to Satyawada Rama Rao.

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Lamare, A., Rao, S.R. Efficacy of RAPD, ISSR and DAMD markers in assessment of genetic variability and population structure of wild Musa acuminata colla. Physiol Mol Biol Plants 21, 349–358 (2015). https://doi.org/10.1007/s12298-015-0295-1

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