Skip to main content
Log in

Variation and Intraspecific Relationships in Indian Wild Musa balbisiana (BB) Population as Evidenced by Random Amplified Polymorphic DNA

  • Published:
Genetic Resources and Crop Evolution Aims and scope Submit manuscript

Abstract

Sixteen collections of the wild Musa species, Musa balbisiana Colla collected from different regions of India were studied for their intraspecific relationships using random amplified polymorphic DNA (RAPD) markers. Out of 80 primers screened, 34 primers produced reproducible bands and four primers among them showing polymorphic bands were used. In all, 43 DNA fragments were amplified averaging 10.75 per primer. Of these, 31 amplified fragments showed polymorphism (averaging of 7.75 per primer). The extent of polymorphism (74.6%) has indicated the existence of considerable variation at the DNA level within the species. The 16 accessions were clustered into four as against seven clusters obtained through morphotaxonomic characterization. The inter relationships based on geographical origin in comparison with molecular characterization have been discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Anonymous 2002. Recommendations of the promusa workshop of M. balbisiana diversity held at Bangkok, Thailand, 8–10 July 2002.

  • K.V. Bhat R.L. Jarret (1995) ArticleTitleRandom Amplified Polymorphic DNA and genetic diversity in Indian Musa germplasm Genet. Resour. Crop Evol. 42 107–118

    Google Scholar 

  • K.V. Bhat S.R. Bhat K.P.S. Chandel (1992a) ArticleTitleSurvey of isozyme polymorphism for clonal identification in Musa. I. Esteraseacid phosphatase and catalase J. Hort. Sci. 67 501–507 Occurrence Handle1:CAS:528:DyaK38XmtV2jsLk%3D

    CAS  Google Scholar 

  • K.V. Bhat S.R. Bhat K.P.S. Chandel (1992b) ArticleTitleSurvey of isozyme polymorphism for clonal identification in Musa.II. Peroxidase, superoxide dismutase, shikimate dehydrogenase and malate dehydrogenase J. Hort. Sci. 67 737–744 Occurrence Handle1:CAS:528:DyaK3sXhsVent7s%3D

    CAS  Google Scholar 

  • F. Carreel (1994) Etude de la diversité des bananiers (genre Musa) à l aide des marqueurs RFLP Thèse de la Institut National Agronomique Paris–Grignon 90

    Google Scholar 

  • N.J. Gawal R.L. Jarret (1991) ArticleTitleCytoplasmic genetic diversity in banana and plantain Euphytica 52 19–23

    Google Scholar 

  • E.C. Howell H.J. Newbury R.L. Swennen L.A. Withers B.V. Ford Lioyd (1994) ArticleTitleThe use of RAPD for identifying and classifying Musa germplasm Genome 37 328–332 Occurrence Handle1:CAS:528:DyaK2cXmvFeru70%3D Occurrence Handle8200520

    CAS  PubMed  Google Scholar 

  • IPGRI–INIBAP/CIRAD 1996. Descriptor for Banana (Musa ssp.).

  • D. Kaemmer R. Afza G. Kaw F.J. Novak (1992) ArticleTitleOligonucleotide and amplification fingerprinting of wild species and cultivars of banana Musa spp.) Biotechnology 10 1030–1035 Occurrence Handle1:CAS:528:DyaK3sXhslSmtQ%3D%3D Occurrence Handle1369000

    CAS  PubMed  Google Scholar 

  • F.J. Rohlf (1990) NTSYS-PC. Numerical taxonomy and multivariate analysis system Exeter Software New York

    Google Scholar 

  • N.W. Simmonds (1959) Bananas Longmans, Green and Co. Ltd. London

    Google Scholar 

  • N.W. Simmonds (1962) The Evolution of the Bananas Longmans, Green and Co. Ltd. London

    Google Scholar 

  • Singh H.P. and Uma S. 2000. Genetic diversity of banana in India. In: Singh H.P and Chadha K.L. (eds), The Proceedings of the Conference on Challenges for Banana Production and Utilization in 21st Century. pp. 136–156.

  • R.C. Sotto R.C. Rabara (2000) ArticleTitleMorphological diversity of Musa balbisiana Colla in the Philippines Infomusa 9 IssueID2 28–30

    Google Scholar 

  • S. Uma S. Sathiamoorthy (2002) Names and Synonyms of Bananas and Plantains in India National Research Centre for Banana Tiruchirappalli, India 60

    Google Scholar 

  • J.G.K. Williams A.R. Kubelik K.J. Livak J.A. Rafalski S.V. Tingey (1990) ArticleTitleDNA polymorphisms amplified by arbitrary primers are useful as genetic markers Nucleic Acid Res. 18 6531–6536 Occurrence Handle1:CAS:528:DyaK3MXjslWmsA%3D%3D Occurrence Handle1979162

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to S. Uma.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Uma, S., Siva, S.A., Saraswathi, M.S. et al. Variation and Intraspecific Relationships in Indian Wild Musa balbisiana (BB) Population as Evidenced by Random Amplified Polymorphic DNA. Genet Resour Crop Evol 53, 349–355 (2006). https://doi.org/10.1007/s10722-004-0576-y

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10722-004-0576-y

Keywords

Navigation