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Nitric oxide and brassinosteroids enhance chromium stress tolerance in Glycine max L. (Merr.) by modulating antioxidative defense and glyoxalase systems

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Abstract

Chromium (Cr) contamination of agricultural soils is a major threat to human and plant health worldwide and causes reductions in plant growth and crop yields. 24-epibrassinolide (EBL) and nitric oxide (NO) have been shown to ameliorate the reductions in growth caused by the stresses induced by heavy metals; however, the interactions between EBL and NO on the alleviation of Cr-induced phytotoxicity have been poorly studied. Hence, this study was undertaken to examine any beneficial effects of EBL (0.01 µM) and NO (100 µM), applied alone or in combination, on the mitigation of stress induced by Cr (100 µM) in soybean seedlings. Although EBL and NO applied alone reduced the toxic effects of Cr, the combined treatment had the greatest effect. Mitigation of Cr intoxication occurred via reduced Cr uptake and translocation and by ameliorating reductions in water contents, light-harvesting pigments, and other photosynthetic parameters. In addition, the two hormones increased the activity of enzymatic and non-enzymatic defense mechanisms increasing the scavenging of reactive oxygen species, thereby reducing membrane damage and electrolyte leakage. Furthermore, the hormones reduced the accumulation of the toxic compound, methylglyoxal, by amplifying activities of glyoxalase I and glyoxalase II. Thus, applications of NO and EBL can significantly mitigate Cr-phytotoxicity when cultivating soybean plants in Cr-contaminated soils. However, further more-in depth studies including field investigations parallel with calculations of cost to profit ratios and yield losses are requested to validate the effectiveness of NO and/or EBL for remediation agents in Cr-contaminated soils with using key biomarkers (i.e., oxidative stress, antioxidant defense, and osmoprotectants) involved in the uptake, accumulation, and attenuation of Cr toxicity tested in our study.

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Funding

This research was supported by the Hainan Provincial Joint Project of Sanya Yazhou Bay Science and Technology City (No. 320LH032), the Hainan Provincial Natural Science Foundation of China (No. 322CXTD522), the National Natural Science Foundation of China (Zhejiang Provincial Natural Science Foundation (No. LY21C130006) and Collaborative Innovation Center for Modern Crop Production co-sponsored by Province and Ministry (CIC-MCP).

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  1. Farwa Basit and Ji Tao contributed equally to this work.

Authors and Affiliations

  1. Hainan Research Institute, Zhejiang University, Sanya, 572025, China

    Farwa Basit, Jin Hu & Yajing Guan

  2. Seed Science Center, The Advanced Seed Institute, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China

    Farwa Basit, Ji Tao, Jianyu An, Xiaoyu Song, Jin Hu & Yajing Guan

  3. Department of Agronomy, Faculty of Agriculture, Mansoura University, Mansoura, 35516, Egypt

    Mohamed Salah Sheteiwy

  4. School of Science, Western Sydney University, Locked Bag 1797, Penrith, NSW, 2751, Australia

    Paul Holford

  5. Institute of Plant Genetics, Breeding and Biotechnology, University of Life Sciences in Lublin, Lublin, Poland

    Izabela Jośko

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  1. Farwa Basit
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Contributions

Farwa Basit: conceptualization, investigation, and writing—original draft. Yajing Guan, Jin Hu, and Farwa Basit: conceptualization and investigation. Ji Tao and Mohamed Salah Sheteiwy: validation, writing, reviewing, and editing. Izabela Jośko and Yajing Guan: investigation and formal analysis. Mohamed Salah Sheteiwy, Paul Holford, and Jianyu An: investigation. Farwa Basit and Izabela Jośko: writing, reviewing, and editing.

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Correspondence to Yajing Guan.

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Basit, F., Tao, J., An, J. et al. Nitric oxide and brassinosteroids enhance chromium stress tolerance in Glycine max L. (Merr.) by modulating antioxidative defense and glyoxalase systems. Environ Sci Pollut Res 30, 51638–51653 (2023). https://doi.org/10.1007/s11356-023-25901-0

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