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Effect of nickel on antioxidative enzyme activities, proline and chlorophyll contents in wheat shoots

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Biologia Plantarum

Abstract

Effect of two Ni concentrations (10 and 200 μM) on growth, Ni accumulation, chlorophyll and proline contents, relative water content (RWC) as well as the activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) and glutathione S-transferase (GST) were studied in shoots of wheat plants. Treatments caused a considerable accumulation of Ni in the shoots. However, exposure of plants to 10 μM Ni did not lead to significant alterations in shoot growth except for a slight increase in fresh mass. The other parameters studied were not affected by treatment of plants with 10 μM Ni. In contrast, 200 μM Ni caused inhibition of shoot growth, a decline in RWC and chlorophyll content, accumulation of proline and occurrence of visible symptoms of Ni toxicity. The activities of SOD and CAT decreased in response to 200 μM Ni. Conversely, several-fold enhancements of POD and GST activities were observed following the 3rd day of 200 μM Ni treatment.

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Abbreviations

CAT:

catalase

CDNB:

1-chloro-2,4-dinitrobenzene

GSH:

reduced glutathione

GST:

glutathione S-transferase

NBT:

nitroblue tetrazolium

POD:

peroxidase

ROS:

reactive oxygen species

RWC:

relative water content

SOD:

superoxide dismutase

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Authors and Affiliations

  1. Department of Plant Physiology and Biochemistry, University of Łódź, Banacha 12/16, PL-90237, Łódź, Poland

    E. Gajewska & M. Skłodowska

  2. Department of Industrial Microbiology and Biotechnology, University of Łódź, Banacha 12/16, PL-90237, Łódź, Poland

    M. Słaba

  3. Laboratory of Computer and Analytical Techniques, University of Łódź, Banacha 12/16, PL-90237, Łódź, Poland

    J. Mazur

Authors
  1. E. Gajewska
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  2. M. Skłodowska
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  3. M. Słaba
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  4. J. Mazur
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Correspondence to E. Gajewska.

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Gajewska, E., Skłodowska, M., Słaba, M. et al. Effect of nickel on antioxidative enzyme activities, proline and chlorophyll contents in wheat shoots. Biol Plant 50, 653–659 (2006). https://doi.org/10.1007/s10535-006-0102-5

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  • DOI: https://doi.org/10.1007/s10535-006-0102-5

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