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Plant regeneration from mature embryo of commercial Indian bread wheat (Triticum aestivum L.) cultivars

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Abstract

A simple, efficient, reproducible and comparatively genotype-independent in vitro plant regeneration protocol was developed for ten commercial Indian bread wheat cultivars using mature embryos as the explants. Three different auxins and five different combinations of growth regulators in a modified Murashige and Skoog’s basal medium were assessed for their effect on callus induction and plant regeneration, respectively, in a high yielding and widely grown cultivar, PBW-343. The optimized conditions were further evaluated with nine other commercial cultivars. A simple novel approach of physical isolation of regenerable calli from non regenerable structures during the early callus phase was used to improve plant regeneration. Callus induced on 2.0 mg-1 2,4-dichlorophenoxyacetic acid (2,4-D) showed a regeneration frequency of 86 % with 7.5 shoots per explants on hormone-free medium. A considerable improvement in the regeneration frequency (up to 97 %) and the average of shoots (19 shoots per explants) was obtained with a combination of thidiazuron (TDZ) and 2,4-D.

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Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

Dicamba:

3,6-dichloro-2-methoxybenzoic acid

Picloram:

4-amino-3, 5-trichoropiclinic acid

TDZ:

Thidiazuron

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Acknowledgements

The authors are grateful to UGC for the financial support and NBPGR, New Delhi and DWR, Karnal, India for the supply of seeds of wheat cultivars. SSP is thankful to CSIR, New Delhi, India for the award of the Senior Research Fellowship.

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Authors and Affiliations

  1. Centre for Biotechnology, M. D. University, Rohtak, 124001, India

    Sanjay Singh Parmar, Manish Sainger, Darshna Chaudhary & Pawan K. Jaiwal

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  1. Sanjay Singh Parmar
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  2. Manish Sainger
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  3. Darshna Chaudhary
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  4. Pawan K. Jaiwal
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Correspondence to Pawan K. Jaiwal.

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Parmar, S.S., Sainger, M., Chaudhary, D. et al. Plant regeneration from mature embryo of commercial Indian bread wheat (Triticum aestivum L.) cultivars. Physiol Mol Biol Plants 18, 177–183 (2012). https://doi.org/10.1007/s12298-012-0101-2

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