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Primary studies on tissue culture from mature embryos in diploid and tetraploid wheat

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  • Published:
Frontiers of Agriculture in China

Abstract

Using mature embryos (MEs) as the explants, the callus induction, embryogenic callus differentiation, plantlet regeneration and culture efficiency in diploid and tetraploid wheat of four genotypes were studied. The tested four genotypes wheat included cultivable emmer wheat (Triticum dicoccum Schuble), durum wheat (Triticum durum Desf.) and the common wheat progenitors Triticum dicoccoides and Triticum aegilopides. Results indicated that there were significant differences in the efficiency of callus induction, callus differentiation and plant regeneration among the tested genotypes. The efficiency of differentiation and regeneration shows strong genotype dependence. The rates of callus induction, embryogenic callus differentiation, plantlet regeneration and culture efficiency respectively were 95.00%, 90.00%, 32.40%, and 27.70% in cultivable emmer wheat, which were significantly higher than other tested genotypes. Therefore, this study has provided a basis for genetic transformation, gene cloning and molecular plant breeding in wheat and other related species.

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Authors and Affiliations

  1. Department of Life Science of Heze University, Heze, 274015, China

    Ruiming Bi & Honggang Wang

  2. Subcenter of National Wheat Improvement Center, the National Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an, 271018, China

    Ruiming Bi & Honggang Wang

Authors
  1. Ruiming Bi
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  2. Honggang Wang
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Correspondence to Honggang Wang.

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Translated from Molecular Plant Breeding, 2007, 5(4): 480–484 [译自: 分子植物育种]

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Bi, R., Wang, H. Primary studies on tissue culture from mature embryos in diploid and tetraploid wheat. Front. Agric. China 2, 262–265 (2008). https://doi.org/10.1007/s11703-008-0038-y

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  • DOI: https://doi.org/10.1007/s11703-008-0038-y

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