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In vitro regeneration of cereals based on multiple shoot induction from mature embryos in response to thidiazuron

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Abstract

The in vitro competency of mature cereal embryos (winter, spring and durum wheats, oat, barley and triticale) was assessed for direct multiple shoot production on culture media containing the plant growth regulators, thidiazuron (TDZ) and/or 6–benzylaminopurine (BAP). Mature embryos of CDC Dancer oat showed the best response, with 69 shoots per explant on culture medium containing a combination of 4.5 μM TDZ and 4.4 μM BAP. TDZ alone induced about 16 shoots per explant from the oat. Among the wheat genotypes, durum wheat showed the most number of shoots (35) per explant on culture medium containing 4.5 μM of TDZ and 4.4 μM of BAP. With TDZ alone, shoot regeneration for durum wheat ranged from 27–32 shoots per explant. The regeneration frequency from the three winter wheat genotypes ranged from 11–25 shoots per explant and was highest on culture medium containing 9.1 μM TDZ and 4.4 μM BAP. The latter culture medium was also effective for a triticale genotype, inducing 34 shoots per explant. The regeneration from mature embryos of barley genotypes ranged from 5–9 shoots per explant. The mature embryos of all the cereals tested could be used for in vitro regeneration with TDZ and TDZ+BAP combinations.

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Abbreviations

2,4-d :

2,4-dichlorophenoxyacetic acid

BAP:

6-benzylaminopurine

TDZ:

thidiazuron

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Acknowledgements

Research support from the SECAN and WGRF collaborative project, Canada-Saskatchewan Agri-Food Innovation Fund, Genome Canada/Genome Prairie, the Natural Sciences and Engineering Research Council and National Research Council Canada collaborative project, Canada Research Chair and Canada Foundation for Innovation, is gratefully acknowledged. Seeds of AC Nanda were obtained through the courtesy of R. S. Sadasiviah, Agriculture and Agri-Food Canada, Lethbridge, Alberta.

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Authors and Affiliations

  1. Department of Plant Sciences/Crop Development Centre, University of Saskatchewan, 51 Campus Drive, S7N 5A8, Saskatoon, Saskatchewan, Canada

    Seedhabadee Ganeshan, Sanjay V. Chodaparambil, Monica Båga, D. Brian Fowler, Pierre Hucl, Brian G. Rossnagel & Ravindra N. Chibbar*

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  1. Seedhabadee Ganeshan
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  2. Sanjay V. Chodaparambil
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  3. Monica Båga
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  4. D. Brian Fowler
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  5. Pierre Hucl
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  6. Brian G. Rossnagel
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  7. Ravindra N. Chibbar*
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Correspondence to Ravindra N. Chibbar*.

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Ganeshan, S., V. Chodaparambil, S., Båga, M. et al. In vitro regeneration of cereals based on multiple shoot induction from mature embryos in response to thidiazuron. Plant Cell Tiss Organ Cult 85, 63–73 (2006). https://doi.org/10.1007/s11240-005-9049-z

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  • DOI: https://doi.org/10.1007/s11240-005-9049-z

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