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Role of kinetin and a morphactin in leaf disc senescence of Raphanus sativus L. under low light

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Abstract

The effects of exogenous application of plant growth regulators (PGRs) like kinetin and a morphactin were investigated in leaf discs obtained from detached senescent Raphanus sativus L. Chetki long leaves under continuous light with fluorescent tube of 8.12 μmol photon m-2 s-1 PFD. Senescence induced changes were characterized by a gradual breakdown of chlorophylls, carotenoids and protein whereas, POD (peroxidase) and protease activity; and total sugars revealed an increment. Application of kinetin (KN) and a morphactin (MOR; chlorflurenol methyl ester-CME 74050) found to be effective in senescence delay, by minimizing breakdown of chlorophylls and carotenoids; and by bringing down peroxidase and protease activity, and sugar accumulation. Although both PGR’s were able to minimize senescence, their higher concentration found to be more effective than the lower one.

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Abbreviations

PGRs:

Plant growth regulators

PFD:

Photon flux density

POD:

Peroxidase

KN:

Kinetin

MOR:

Morphactin (Chlorflurenol methyl ester-CME-74050)

SA:

Salicylic acid

CaCO3 :

Calcium carbonate

HXKs,HXK1:

Hexose kinase

EIN3:

Ethylene insensitive3

ipt:

Isopentenyl transferase

SAG12:

Senescence associated gene12

References

  • Arnon DI (1949) Copper enzymes in isolated chloroplasts: polyphenoloxidase in Beta vulgaris. Plant Physiol 24:1–15

    Article  PubMed  CAS  Google Scholar 

  • Arteca RN (1996) Plant growth substances: principles and applications. Springer, pp 332

  • Balibrea Lara M, Gonzalez Garcia M, Fatima T, Ehneß R, Kyun Lee T, Proels R, Tanner W, Roitsch T (2004) Extracellular invertase is an essential component of cytokinin-mediated delay of senescence. Plant Cell 16:1276–1287

    Article  PubMed  CAS  Google Scholar 

  • Bradford MM (1976) To estimate protein utilizing the principles of protein-dye binding. Anal Biochem 72:248–254

    Article  PubMed  CAS  Google Scholar 

  • Browse J, Somerville CR (1991) Glycerolipid synthesis: biochemistry and regulation. Ann Rev Plant Physiol Plant Mol Biol 42:467–506

    Article  CAS  Google Scholar 

  • Chang CJ, Kao CH (1998) H2O2 metabolism during senescence of rice leaves: changes in enzyme activities in light and darkness. Plant Growth Regul 25:11–15

    Article  CAS  Google Scholar 

  • Chernyad’s II (2000) Ontogenetic changes in the photosynthetic apparatus and effects of cytokinins. Appl Environ Microbiol 36:527–539

    Google Scholar 

  • Costa ML, Civello PM, Chaves AR, Martinez GA (2005) Effect of ethephon and 6-benzylaminopurine on chlorophyll degradaing enzyme and a peroxidase –linked chlorophyll bleaching during post harvest senescence of broccoli (Brassica oleracea L.) at 20 degrees C. Postharvest Biol Tech 35:191–199

    Article  CAS  Google Scholar 

  • Gan S, Amasino RM (1995) Inhibition of leaf senescence by auto regulated production of cytokinin. Science 70:1986–1988

    Article  Google Scholar 

  • Gan S, Amasino RM (1997) Making sense of senescence. Plant Physiol 113:313–319

    PubMed  CAS  Google Scholar 

  • Grover A, Mohanty P (1993) Leaf senescence-induced alteration in structure and function of higher plant chloroplasts. In: Abrol YP, Mohanty P, Govindjee (eds) Photosynthesis: photoreaction to plant productivity. Kluwer, London, pp 225–255

    Chapter  Google Scholar 

  • Hart FL, Fisher HJ (1971) Modern food analysis. Springer, New york

    Google Scholar 

  • Hensel LL, Grbic Baumgarten DA, Bleecker AB (1993) Developmental and age related processes that influence the longevity and senescence of photosynthetic tissues in Arabidopsis. Plant Cell 5:553–564

    Article  PubMed  CAS  Google Scholar 

  • Hellgren LI, Sandelius AS (2001) Age dependent variation in membrane lipid synthesis in leaves of garden pea (Pisum sativum). J Exp Bot 365:2275–2282

    Article  Google Scholar 

  • Hidema J, Makino A, Kurita Y, Mae T, Ojima K (1992) Changes in the levels of chlorophyll and light harvesting chlorophyll a/b protein of PS II in rice leaves aged under different irradiance from full expansion through senescence. Plant Cell Physiol 33:1209–1214

    CAS  Google Scholar 

  • Hodges DM, Forney CF (2000) The effect of ethylene, depressed oxygen and elevated carbon dioxide on antioxidant profile of senescing spinach leaves. J Exp Bot 51:645–655

    Article  PubMed  CAS  Google Scholar 

  • van Hoeberichts FA, Doorn WG, Vorst O, van Hall RD, Wordragen MF (2007) Sucrose prevents up-regulation of sensececent-associated genes in carnation petals. J Exp Bot 58:2873–2885

    Article  PubMed  CAS  Google Scholar 

  • Holden M (1965) Chlorophylls. In: Goodwin TW (ed) Chemistry and biochemistry of plant pigments. Academic, London, pp 462–488

    Google Scholar 

  • Hortensteiner S, Feller U (2002) Nitrogen metabolism and remobilization during senescence. J Exp Bot 53:927–937

    Article  PubMed  CAS  Google Scholar 

  • Jakhar S, Mukherjee D (2006) Chloroplast pigments, free and bound amino acids, activities of protease and peroxidase during development and senescence of attached nodal leaves of Cajanus cajan L. J Plant Biol 33:125–132

    CAS  Google Scholar 

  • Kanazawa S, Sano S, Koshiba T, Ushimaru T (2000) Changes in antioxidative enzymes in cucumber cotyledons during dark-induced senescence. Physiol Plant 109:211–216

    Article  CAS  Google Scholar 

  • Kar M, Streh P, Hertwig B, Feierabend J (1993) Sensitivity to photo damage increases during senescence in excised leaves. J Plant Physiol 141:538–544

    CAS  Google Scholar 

  • Kumar R (1990) Senescence regulation in pigeon pea with reference to water stress and growth substances. Ph.D. Thesis. Kurukshetra University, Kurukshetra

  • Kumar R, Mukherjee D (1992) Effect of kinetin on growth and yield of pigeonpea. Geobios 19:204–208

    CAS  Google Scholar 

  • Kuraishi S (1968) The effect of kinetin on protein level of Brassica leaf discs. Physiologia Plant 21:78

    Article  CAS  Google Scholar 

  • Lu C, Zhang J (1998a) Changes in photosystem II function during senescence of wheat leaves. Physiol Plant 104:239–247

    Article  CAS  Google Scholar 

  • Lu C, Zhang J (1998b) Modifications in photosystems II photochemistry in senescent leaves of maize plants. J Exp Bot 49:1671–1679

    Article  CAS  Google Scholar 

  • Maehly AC (1954) Determination of peroxidase activity. In: Glick D (ed) Methods of biochemical analysis. Interscience Publications, Inc, New York, pp 385–386

    Google Scholar 

  • Martin C, Thimann KV (1972a) The role of protein synthesis in the senescence of leaves I. The formation of proteases. Plant Physiol 49:64–71

    Article  PubMed  CAS  Google Scholar 

  • Martin C, Thimann KV (1972b) The role of protein synthesis in the senescence of leaves II. The influence of amino acids on senescence. Plant Physiol 50:432–437

    Article  PubMed  CAS  Google Scholar 

  • Matile P, Hortensteiner S, Thomas H, Krautler B (1996) Chlorophyll breakdown in senescent leaves. Plant Physiol 112:1403–1409

    PubMed  CAS  Google Scholar 

  • McCabe MS, Garratt LC, Schepers F, Jordi WJRM, Stoopen GM, van Davelaar E, Rhijk JHA, Power B, Davey MR (2001) Effects of PSAG 12-IPT gene expression on development and senescence in transgenic lettuce. Plant Physiol 127:505–516

    Article  PubMed  CAS  Google Scholar 

  • McKenzie MJ, Mett V, Reynolds PHS, Jameson PE (1998) Controlled cytokinin production in transgenic tobacco using a copper inducible promoter. Plant Physiol 116:969–977

    Article  PubMed  CAS  Google Scholar 

  • Mukherjee D, Jakhar S (2009) Combined application of serine and kinetin regulates leaf disc senescence in Spinacia oleracea L. Indian J of Plant Physiol 14:229–238

    CAS  Google Scholar 

  • Mukherjee D, Kumar R (2007) Kinetin regulates plant growth and biochemical changes during maturation and senescence of leaves, flowers and pods of Cajanus cajan L. Biol Plant 51:80–85

    Article  CAS  Google Scholar 

  • Mukherjee D, Rao KUM (1993) Protease activity in leaves, flowers and pods of Cajanus cajan during maturation and senescence. J Plant Physiol Biochem 20:45–48

    Google Scholar 

  • Mukherjee D, Jain VK, Gupta VK (1983) Moprhogenetic and biochemical changes in Lycopersicon esculentum after the treatment with a morphactin. Plant Physiol Biochem 10:96–107

    Google Scholar 

  • Nooden BD, Nooden SM (1985) Effect of morphactin and other auxin transport inhibitors on soybean senescence and pod development. Plant Physiol 78:263–266

    Article  PubMed  CAS  Google Scholar 

  • Otegui MS, Noh Y-S, Martinez DE, Villa Petroff MG, Staehelin LA, Amasino RM, Guiamet JJ (2005) Senescence-associated vacuoles with intense proteolytic activity develop in leaves of Arabidopsis and soybean. Plant 41:836–844

    Google Scholar 

  • Paranjothy K, Wareing PF (1971) The effect of abscisic acid, kinetin, and 5-fluorouracil on ribonucleic acid and protein synthesis in senescing leaf discs. Planta 99:112–119

    Article  CAS  Google Scholar 

  • Ponmeni G, Mukherjee D (1997) Kinetin induced alteration in senescence of red gram leaves. Indian J Plant Physiol (New Series) 2:250–251

    CAS  Google Scholar 

  • Quirino BF, Reiter WD, Amasino RM (2001) One of the two tandem Arabidopsis genes homologous to monosaccharides transporters is senescence associated. Plant Mol Biol 46:447–457

    Article  PubMed  CAS  Google Scholar 

  • Rajbala, Mukherjee D (2004) Senescence regulation in leaf discs of Lycopersicon esculentum and Solanum melongena by kinetin and a morphactin in dark. Eco-Research J Bio-Sciences 31:1–8

    Google Scholar 

  • Rao KUM (1990) Senescence of leaves and reproductive parts of Cajanus cajan L. Ph.D. Thesis, Kurukshetra University, Kurukshetra

  • Rao KUM, Mukherjee D (1990) Some metabolic changes during development and senescence in the leaves of Cajanus cajan L. J Indian Bot Soc 69:311–314

    Google Scholar 

  • Reimerdes EH, Klostermeyer H (1976) Determination of proteolytic activity on caesin substrates. Methods Enzymol 45:26–28

    Article  PubMed  CAS  Google Scholar 

  • Robson PRH, Donnison IS, Wang K, Frame B, Pegg SE, Thomas A, Thomas H (2004) Leaf senescence is delayed in maize expressing the Agrobacterium ipt gene under the control of a novel, maize senescence enhanced promoter. Plant Biotechnol J 2:101–112

    Article  PubMed  CAS  Google Scholar 

  • Rolland F, Moore B, Sheen J (2002) Sugar sensing and signaling in plants. Plant Cell Suppl 5:S185–S205

    Google Scholar 

  • Schneider G (1970) Morphactins: physiology and performance. Ann Rev Plant Physiol 21:499–536

    Article  CAS  Google Scholar 

  • Rolland F, Baena-Gonzalez E, Sheen J (2006) Sugar sensing and signaling in plants: conserved and novel mechanisms. Ann Rev Plant Biol 57:675–709

    Article  CAS  Google Scholar 

  • Smart CM (1994) Gene expression during leaf senescence. New Phytol 126:419–448

    Article  CAS  Google Scholar 

  • Stessman D, Miller A, Spalding M, Rodermel S (2002) Regulation of photosynthesis during Arabidopsis leaf development in continuous light. Photosynth Res 72:27–37

    Article  PubMed  CAS  Google Scholar 

  • Wilhelmova N (1998) The role of oxidative damage in cotyledon ageing. Abstracts J Expt Bot 321:49

    Google Scholar 

  • Van Wingler A, Schawen A, Leegood RC, Lea PJ, Quick W (1998) Regulation of leaf senescence by cytokinin, sugars and light. Plant Physiol 116:329–335

    Article  CAS  Google Scholar 

  • Yamada K, Matsushima R, Nishimura M, Hara-Nishimura I (2001) A slow maturation of a cysteine protease with a granulin domain in the vacuoles of senescing Arabidopsis leaves. Plant Physiol 127:1626–1634

    Article  PubMed  CAS  Google Scholar 

  • Yemm EW, Cocking EC (1955) The determination of amino acids with ninhydrin. Analyst 80:209–213

    Article  CAS  Google Scholar 

Download references

Acknowledgement

Financial assistance received from University Grants Commission, New Delhi to the first author is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Botany, Kurukshetra University, Kurukshetra, 136119, India

    Seema, Mansee Khokhar & Dibakar Mukherjee

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  1. Seema
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  2. Mansee Khokhar
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  3. Dibakar Mukherjee
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Correspondence to Dibakar Mukherjee.

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Seema, Khokhar, M. & Mukherjee, D. Role of kinetin and a morphactin in leaf disc senescence of Raphanus sativus L. under low light. Physiol Mol Biol Plants 17, 247–253 (2011). https://doi.org/10.1007/s12298-011-0077-3

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